(Circular) (Six-base) MAPSORT of: pFN21AE4153.seq Check: 6240 from: 1 to: 6469 pFN21AE4153 6469 bp With 182 enzymes: SgfI * March 28, 2012 13:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3110 3110 Size: 6469 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4279 278 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2486 3154 3812 1937 Size: 549 668 658 4594 Fragments arranged by size: 4594 668 658 549 Acc65I G'GTAC_C Cuts at: 3790 3790 Size: 6469 AclI AA'CG_TT Cuts at: 1563 4596 4969 1563 Size: 3033 373 3063 Fragments arranged by size: 3063 3033 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3566 4532 5916 6184 1632 Size: 1934 966 1384 268 1917 Fragments arranged by size: 1934 1917 1384 966 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6272 AflIII A'CryG_T Cuts at: 1203 2676 5368 6345 1203 Size: 1473 2692 977 1327 Fragments arranged by size: 2692 1473 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 2482 5198 2482 Size: 2716 3753 AleI CACnn'nnGTG Cuts at: 1166 2700 1166 Size: 1534 4935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2889 1184 Size: 1705 4764 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2676 2708 2676 Size: 32 6437 AlwNI CAG_nnn'CTG Cuts at: 4212 5784 4212 Size: 1572 4897 ApaLI G'TGCA_C Cuts at: 4526 5682 4526 Size: 1156 5313 ApoI r'AATT_y Cuts at: 1493 2318 3370 3636 3778 4004 4156 6265 1493 Size: 825 1052 266 142 226 152 2109 1697 Fragments arranged by size: 2109 1697 1052 825 266 226 152 142 AseI AT'TA_AT Cuts at: 160 1032 5023 160 Size: 872 3991 1606 Fragments arranged by size: 3991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 3794 1870 Size: 73 1851 4545 Fragments arranged by size: 4545 1851 73 BamHI G'GATC_C Cuts at: 3799 4166 3799 Size: 367 6102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3790 5245 618 Size: 325 217 627 2003 1455 1842 Fragments arranged by size: 2003 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2471 3788 1391 Size: 790 290 1317 4072 Fragments arranged by size: 4072 1317 790 290 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 3062 928 Size: 971 138 1025 4335 Fragments arranged by size: 4335 1025 971 138 BbvCI CC'TCA_GC Cuts at: 3299 3299 Size: 6469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3608 3642 4660 4694 1903 Size: 34 1671 34 1018 34 3678 Fragments arranged by size: 3678 1671 1018 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 4361 5571 2002 Size: 2359 1210 2900 Fragments arranged by size: 2900 2359 1210 BclI T'GATC_A Cuts at: 1548 2493 1548 Size: 945 5524 BfrBI ATG'CAT Cuts at: 2297 2548 3238 3352 2297 Size: 251 690 114 5414 Fragments arranged by size: 5414 690 251 114 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5080 136 Size: 107 122 71 631 605 3408 1525 Fragments arranged by size: 3408 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 3886 3886 Size: 6469 Bme1580I G_kGCm'C Cuts at: 1790 4530 5686 1790 Size: 2740 1156 2573 Fragments arranged by size: 2740 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2677 1318 Size: 1359 5110 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2888 3255 4200 Size: 416 53 83 399 24 1471 367 945 Cuts at: 4200 5158 442 Size: 958 1753 Fragments arranged by size: 1753 1471 958 945 416 399 367 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 3641 5129 1239 Size: 926 1476 1488 2579 Fragments arranged by size: 2579 1488 1476 926 Bpu10I CC'TnA_GC Cuts at: 3299 3299 Size: 6469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 2300 4594 5459 5757 2195 Size: 105 2294 865 298 2907 Fragments arranged by size: 2907 2294 865 298 105 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2432 3659 5132 882 Size: 617 933 1227 1473 2219 Fragments arranged by size: 2219 1473 1227 933 617 BsaAI yAC'GTr Cuts at: 493 1204 4260 493 Size: 711 3056 2702 Fragments arranged by size: 3056 2702 711 BsaBI GATnn'nnATC Cuts at: 3418 6463 3418 Size: 3045 3424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2649 2735 4276 4658 Size: 53 83 186 936 1116 86 1541 382 Cuts at: 4658 5293 275 Size: 635 1451 Fragments arranged by size: 1541 1451 1116 936 635 382 186 86 83 53 BsaWI w'CCGG_w Cuts at: 4902 5300 5574 5721 6132 4902 Size: 398 274 147 411 5239 Fragments arranged by size: 5239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2595 2625 3204 3234 1178 Size: 30 165 30 1192 30 579 30 4413 Fragments arranged by size: 4413 1192 579 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6469 BseYI C'CCAG_C Cuts at: 5672 6168 5672 Size: 496 5973 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2079 3144 3537 1163 Size: 573 343 1065 393 4095 Fragments arranged by size: 4095 1065 573 393 343 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3919 4680 5708 1540 Size: 275 186 1918 761 1028 2301 Fragments arranged by size: 2301 1918 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2131 2181 4530 4615 5686 2131 Size: 50 2349 85 1071 2914 Fragments arranged by size: 2914 2349 1071 85 50 BsmI GAATG_Cn' Cuts at: 3980 4073 3980 Size: 93 6376 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2181 2471 3788 4530 4615 5686 Size: 399 341 50 290 1317 742 85 1071 Cuts at: 5686 1391 Size: 2174 Fragments arranged by size: 2174 1317 1071 742 399 341 290 85 50 BspEI T'CCGG_A Cuts at: 6132 6132 Size: 6469 BspHI T'CATG_A Cuts at: 4358 6088 4358 Size: 1730 4739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3110 3824 844 Size: 2266 714 3489 Fragments arranged by size: 3489 2266 714 BsrBI CCG'CTC Cuts at: 1394 1823 4356 1394 Size: 429 2533 3507 Fragments arranged by size: 3507 2533 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2214 2240 2550 2614 3094 4964 5138 Size: 1183 966 26 310 64 480 1870 174 Cuts at: 5138 65 Size: 1396 Fragments arranged by size: 1870 1396 1183 966 480 310 174 64 26 BsrFI r'CCGG_y Cuts at: 1700 1810 5113 5300 1700 Size: 110 3303 187 2869 Fragments arranged by size: 3303 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 3498 4529 5541 3498 Size: 1031 1012 4426 Fragments arranged by size: 4426 1031 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6469 BstBI TT'CG_AA Cuts at: 3640 5273 3640 Size: 1633 4836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6469 BstXI CCAn_nnnn'nTGG Cuts at: 2702 2702 Size: 6469 BstYI r'GATC_y Cuts at: 1972 3139 3799 4166 4551 4568 6020 6106 6118 Size: 1167 660 367 385 17 1452 86 12 Cuts at: 6118 6464 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1167 660 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3208 513 Size: 552 939 1204 3774 Fragments arranged by size: 3774 1204 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2548 487 Size: 683 56 1322 4408 Fragments arranged by size: 4408 1322 683 56 BtsI GCAGTG_nn' Cuts at: 3171 3985 4779 4799 3171 Size: 814 794 20 4841 Fragments arranged by size: 4841 814 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6469 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6434 DraI TTT'AAA Cuts at: 3730 3773 3730 Size: 43 6426 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5476 809 Size: 507 4160 1802 Fragments arranged by size: 4160 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3575 3916 4805 Size: 54 1425 50 275 18 1745 341 889 Cuts at: 4805 6434 8 Size: 1629 43 Fragments arranged by size: 1745 1629 1425 889 341 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3393 4399 1353 Size: 2040 1006 3423 Fragments arranged by size: 3423 2040 1006 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2738 3575 5056 5430 5576 387 Size: 1064 138 1149 837 1481 374 146 1280 Fragments arranged by size: 1481 1280 1149 1064 837 374 146 138 EcoICRI GAG'CTC Cuts at: 2179 2179 Size: 6469 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 3566 3641 4532 5129 5916 6184 Size: 393 533 1401 75 891 597 787 268 Cuts at: 6184 1239 Size: 1524 Fragments arranged by size: 1524 1401 891 787 597 533 393 268 75 EcoRI G'AATT_C Cuts at: 2318 3778 4156 2318 Size: 1460 378 4631 Fragments arranged by size: 4631 1460 378 EcoRV GAT'ATC Cuts at: 2578 2578 Size: 6469 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2054 2054 Size: 6469 FspI TGC'GCA Cuts at: 4975 4975 Size: 6469 HaeII r_GCGC'y Cuts at: 1678 5616 1678 Size: 3938 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 2595 2619 2627 Size: 32 135 32 896 32 95 24 8 Cuts at: 2627 2651 2720 2752 3204 3236 5110 5142 5184 Size: 24 69 32 452 32 1874 32 42 Cuts at: 5184 5216 1373 Size: 32 2626 Fragments arranged by size: 2626 1874 896 452 135 95 69 42 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2487 3007 3813 4059 669 Size: 988 281 549 520 806 246 3079 Fragments arranged by size: 3079 988 806 549 520 281 246 HindIII A'AGCT_T Cuts at: 748 3689 748 Size: 2941 3528 HpaI GTT'AAC Cuts at: 4059 4059 Size: 6469 KpnI G_GTAC'C Cuts at: 3794 3794 Size: 6469 MfeI C'AATT_G Cuts at: 3223 4068 3223 Size: 845 5624 MluI A'CGCG_T Cuts at: 6345 6345 Size: 6469 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5583 5767 1305 Size: 4278 184 2007 Fragments arranged by size: 4278 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3577 10 Size: 54 1425 343 1745 2902 Fragments arranged by size: 2902 1745 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2700 3243 3379 4427 4786 4945 518 Size: 648 1534 543 136 1048 359 159 2042 Fragments arranged by size: 2042 1534 1048 648 543 359 159 136 MspA1I CmG'CkG Cuts at: 1606 3885 4562 5710 5955 1606 Size: 2279 677 1148 245 2120 Fragments arranged by size: 2279 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3208 513 Size: 552 939 1204 3774 Fragments arranged by size: 3774 1204 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NsiI A_TGCA'T Cuts at: 2299 2550 3240 3354 2299 Size: 251 690 114 5414 Fragments arranged by size: 5414 690 251 114 NspI r_CATG'y Cuts at: 1132 1229 3242 3352 3827 5372 1132 Size: 97 2013 110 475 1545 2229 Fragments arranged by size: 2229 2013 1545 475 110 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6469 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6469 PfoI T'CCnGG_A Cuts at: 2181 2181 Size: 6469 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4538 4570 6051 6083 4538 Size: 32 1481 32 4924 Fragments arranged by size: 4924 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6469 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PstI C_TGCA'G Cuts at: 830 3121 3821 830 Size: 2291 700 3478 Fragments arranged by size: 3478 2291 700 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 SacI G_AGCT'C Cuts at: 2181 2181 Size: 6469 SalI G'TCGA_C Cuts at: 1936 2485 3811 1936 Size: 549 1326 4594 Fragments arranged by size: 4594 1326 549 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 4717 6429 4717 Size: 1712 4757 SfcI C'TryA_G Cuts at: 826 1045 3117 3817 4952 5633 5824 826 Size: 219 2072 700 1135 681 191 1471 Fragments arranged by size: 2072 1471 1135 700 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3796 1872 Size: 1924 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 2279 4573 5474 5736 820 Size: 197 926 231 105 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 262 231 197 105 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3352 3827 1132 Size: 2220 475 3774 Fragments arranged by size: 3774 2220 475 SspI AAT'ATT Cuts at: 5 52 4393 5 Size: 47 4341 2081 Fragments arranged by size: 4341 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6469 StyI C'CwwG_G Cuts at: 513 1065 2004 3208 3467 3908 513 Size: 552 939 1204 259 441 3074 Fragments arranged by size: 3074 1204 939 552 441 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4496 4513 4666 4851 5296 325 Size: 1255 2916 17 153 185 445 1498 Fragments arranged by size: 2916 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3502 3562 4715 Size: 274 80 33 51 1443 1525 60 1153 Cuts at: 4715 6427 96 Size: 1712 138 Fragments arranged by size: 1712 1525 1443 1153 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3409 3441 6429 6461 3409 Size: 32 2988 32 3417 Fragments arranged by size: 3417 2988 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6469 XbaI T'CTAG_A Cuts at: 2974 3805 2974 Size: 831 5638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2621 2621 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6469 XmaI C'CCGG_G Cuts at: 1870 3794 1870 Size: 1924 4545 XmnI GAAnn'nnTTC Cuts at: 3108 4598 3108 Size: 1490 4979 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4277 276 Size: 53 83 186 936 2743 2468 Fragments arranged by size: 2743 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI