(Linear) (Six-base) MAPSORT of: KIEE4153.seq Check: 5322 from: 1 to: 1764 KIEE4153.orf With 182 enzymes: SgfI * March 22, 2012 17:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1105 1764 Size: 1105 659 AccI GT'mk_AC Cuts at: 0 481 1149 1764 Size: 481 668 615 Fragments arranged by size: 668 615 481 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1561 1764 Size: 1561 203 AflIII A'CryG_T Cuts at: 0 671 1764 Size: 671 1093 AhdI GACnn_n'nnGTC Cuts at: 0 477 1764 Size: 477 1287 AleI CACnn'nnGTG Cuts at: 0 695 1764 Size: 695 1069 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 884 1764 Size: 884 880 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 671 703 1764 Size: 671 32 1061 Fragments arranged by size: 1061 671 32 ApoI r'AATT_y Cuts at: 0 313 1365 1631 1764 Size: 313 1052 266 133 Fragments arranged by size: 1052 313 266 133 BanII G_rGCy'C Cuts at: 0 176 466 1764 Size: 176 290 1298 Fragments arranged by size: 1298 290 176 BbsI GAAGACnn'nnnn_ Cuts at: 0 32 1057 1764 Size: 32 1025 707 Fragments arranged by size: 1025 707 32 BbvCI CC'TCA_GC Cuts at: 0 1294 1764 Size: 1294 470 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1603 1637 1764 Size: 1603 34 127 Fragments arranged by size: 1603 127 34 BclI T'GATC_A Cuts at: 0 488 1764 Size: 488 1276 BfrBI ATG'CAT Cuts at: 0 292 543 1233 1347 1764 Size: 292 251 690 114 417 Fragments arranged by size: 690 417 292 251 114 BmgBI CAC'GTC Cuts at: 0 672 1764 Size: 672 1092 BmrI ACTGGGnnnn_n' Cuts at: 0 883 1250 1764 Size: 883 367 514 Fragments arranged by size: 883 514 367 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 1636 1764 Size: 160 1476 128 Fragments arranged by size: 1476 160 128 Bpu10I CC'TnA_GC Cuts at: 0 1294 1764 Size: 1294 470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 295 1764 Size: 190 105 1469 Fragments arranged by size: 1469 190 105 BsaI GGTCTCn'nnnn_ Cuts at: 0 427 1654 1764 Size: 427 1227 110 Fragments arranged by size: 1227 427 110 BsaBI GATnn'nnATC Cuts at: 0 1413 1764 Size: 1413 351 BsaHI Gr'CG_yC Cuts at: 0 644 730 1764 Size: 644 86 1034 Fragments arranged by size: 1034 644 86 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 590 620 1199 1229 1764 Size: 590 30 579 30 535 Fragments arranged by size: 590 579 535 30 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 1139 1532 1764 Size: 74 1065 393 232 Fragments arranged by size: 1065 393 232 74 BsiHKAI G_wGCw'C Cuts at: 0 126 176 1764 Size: 126 50 1588 Fragments arranged by size: 1588 126 50 Bsp1286I G_dGCh'C Cuts at: 0 126 176 466 1764 Size: 126 50 290 1298 Fragments arranged by size: 1298 290 126 50 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1105 1764 Size: 1105 659 BsrDI GCAATG_nn' Cuts at: 0 209 235 545 609 1089 1764 Size: 209 26 310 64 480 675 Fragments arranged by size: 675 480 310 209 64 26 BssSI C'ACGA_G Cuts at: 0 1493 1764 Size: 1493 271 BstBI TT'CG_AA Cuts at: 0 1635 1764 Size: 1635 129 BstXI CCAn_nnnn'nTGG Cuts at: 0 697 1764 Size: 697 1067 BstYI r'GATC_y Cuts at: 0 1134 1764 Size: 1134 630 BtgI C'CryG_G Cuts at: 0 1203 1764 Size: 1203 561 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 543 1764 Size: 543 1221 BtsI GCAGTG_nn' Cuts at: 0 1166 1764 Size: 1166 598 DraI TTT'AAA Cuts at: 0 1725 1764 Size: 1725 39 EaeI y'GGCC_r Cuts at: 0 1570 1764 Size: 1570 194 EarI CTCTTCn'nnn_ Cuts at: 0 1388 1764 Size: 1388 376 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 733 1570 1764 Size: 733 837 194 Fragments arranged by size: 837 733 194 EcoICRI GAG'CTC Cuts at: 0 174 1764 Size: 174 1590 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 1561 1636 1764 Size: 160 1401 75 128 Fragments arranged by size: 1401 160 128 75 EcoRI G'AATT_C Cuts at: 0 313 1764 Size: 313 1451 EcoRV GAT'ATC Cuts at: 0 573 1764 Size: 573 1191 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 49 1764 Size: 49 1715 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 463 495 590 614 622 646 715 747 Size: 463 32 95 24 8 24 69 32 Cuts at: 747 1199 1231 1764 Size: 452 32 533 Fragments arranged by size: 533 463 452 95 69 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 0 482 1002 1764 Size: 482 520 762 Fragments arranged by size: 762 520 482 HindIII A'AGCT_T Cuts at: 0 1684 1764 Size: 1684 80 MfeI C'AATT_G Cuts at: 0 1218 1764 Size: 1218 546 MscI TGG'CCA Cuts at: 0 1572 1764 Size: 1572 192 MslI CAynn'nnrTG Cuts at: 0 695 1238 1374 1764 Size: 695 543 136 390 Fragments arranged by size: 695 543 390 136 NcoI C'CATG_G Cuts at: 0 1203 1764 Size: 1203 561 NsiI A_TGCA'T Cuts at: 0 294 545 1235 1349 1764 Size: 294 251 690 114 415 Fragments arranged by size: 690 415 294 251 114 NspI r_CATG'y Cuts at: 0 1237 1347 1764 Size: 1237 110 417 Fragments arranged by size: 1237 417 110 PfoI T'CCnGG_A Cuts at: 0 176 1764 Size: 176 1588 PstI C_TGCA'G Cuts at: 0 1116 1764 Size: 1116 648 SacI G_AGCT'C Cuts at: 0 176 1764 Size: 176 1588 SalI G'TCGA_C Cuts at: 0 480 1764 Size: 480 1284 SfcI C'TryA_G Cuts at: 0 1112 1764 Size: 1112 652 SmlI C'TyrA_G Cuts at: 0 169 274 1764 Size: 169 105 1490 Fragments arranged by size: 1490 169 105 SphI G_CATG'C Cuts at: 0 1347 1764 Size: 1347 417 StyI C'CwwG_G Cuts at: 0 1203 1462 1764 Size: 1203 259 302 Fragments arranged by size: 1203 302 259 TatI w'GTAC_w Cuts at: 0 1497 1557 1764 Size: 1497 60 207 Fragments arranged by size: 1497 207 60 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1404 1436 1764 Size: 1404 32 328 Fragments arranged by size: 1404 328 32 XbaI T'CTAG_A Cuts at: 0 969 1764 Size: 969 795 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 616 1764 Size: 616 1148 XmnI GAAnn'nnTTC Cuts at: 0 1103 1764 Size: 1103 661 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AleI AlfI AloI ApoI BanII BbsI BbvCI BcgI BclI BfrBI BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BsgI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MscI MslI NcoI NsiI NspI PfoI PstI SacI SalI SfcI SmlI SphI StyI TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BciVI BglI BglII BlpI Bme1580I BmtI BplI BsaAI BsaWI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI EcoNI EcoO109I FseI FspI FspAI HaeII HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I XhoI XmaI ZraI