(Circular) (Six-base) MAPSORT of: pFN21AE2357.seq Check: 6464 from: 1 to: 11650 pFN21AE2357 11650 bp With 182 enzymes: SgfI * July 22, 2011 10:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6746 6746 Size: 11650 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9460 278 Size: 53 83 186 936 7924 2468 Fragments arranged by size: 7924 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3222 4236 4260 8993 1937 Size: 1285 1014 24 4733 4594 Fragments arranged by size: 4733 4594 1285 1014 24 Acc65I G'GTAC_C Cuts at: 5851 6665 8971 5851 Size: 814 2306 8530 Fragments arranged by size: 8530 2306 814 AclI AA'CG_TT Cuts at: 1563 9777 10150 1563 Size: 8214 373 3063 Fragments arranged by size: 8214 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3146 3297 3591 3747 3774 4468 4527 4647 Size: 1514 151 294 156 27 694 59 120 Cuts at: 4647 4752 5559 6468 7179 7449 8484 9713 11097 Size: 105 807 909 711 270 1035 1229 1384 Cuts at: 11097 11365 1632 Size: 268 1917 Fragments arranged by size: 1917 1514 1384 1229 1035 909 807 711 694 294 270 268 156 151 120 105 59 27 AflII C'TTAA_G Cuts at: 820 1017 2234 820 Size: 197 1217 10236 Fragments arranged by size: 10236 1217 197 AflIII A'CryG_T Cuts at: 1203 2168 4048 5223 7669 10549 11526 1203 Size: 965 1880 1175 2446 2880 977 1327 Fragments arranged by size: 2880 2446 1880 1327 1175 977 965 AgeI A'CCGG_T Cuts at: 4255 10481 4255 Size: 6226 5424 AhdI GACnn_n'nnGTC Cuts at: 3011 7673 10379 3011 Size: 4662 2706 4282 Fragments arranged by size: 4662 4282 2706 AleI CACnn'nnGTG Cuts at: 1166 7803 1166 Size: 6637 5013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5231 5955 7605 1184 Size: 4047 724 1650 5229 Fragments arranged by size: 5229 4047 1650 724 AlwNI CAG_nnn'CTG Cuts at: 3881 4592 4775 8092 9393 10965 3881 Size: 711 183 3317 1301 1572 4566 Fragments arranged by size: 4566 3317 1572 1301 711 183 ApaLI G'TGCA_C Cuts at: 4452 9707 10863 4452 Size: 5255 1156 5239 Fragments arranged by size: 5255 5239 1156 ApoI r'AATT_y Cuts at: 1493 2246 2447 2572 3089 3467 3505 4676 5015 Size: 753 201 125 517 378 38 1171 339 Cuts at: 5015 5081 5169 5686 6009 6442 7163 7609 8276 Size: 66 88 517 323 433 721 446 667 Cuts at: 8276 8396 8743 8959 9185 9337 11446 1493 Size: 120 347 216 226 152 2109 1697 Fragments arranged by size: 2109 1697 1171 753 721 667 517 517 446 433 378 347 339 323 226 216 201 152 125 120 88 66 38 AseI AT'TA_AT Cuts at: 160 1032 2316 3266 10204 160 Size: 872 1284 950 6938 1606 Fragments arranged by size: 6938 1606 1284 950 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11650 AvaI C'yCGr_G Cuts at: 1870 1943 3009 8975 1870 Size: 73 1066 5966 4545 Fragments arranged by size: 5966 4545 1066 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5709 5742 6069 6102 6456 6489 7956 7989 5709 Size: 33 327 33 354 33 1467 33 9370 Fragments arranged by size: 9370 1467 354 327 33 33 33 33 BamHI G'GATC_C Cuts at: 3937 6744 8980 9347 3937 Size: 2807 2236 367 6240 Fragments arranged by size: 6240 2807 2236 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4564 5851 6665 8971 10426 Size: 325 217 627 2777 1287 814 2306 1455 Cuts at: 10426 618 Size: 1842 Fragments arranged by size: 2777 2306 1842 1455 1287 814 627 325 217 BanII G_rGCy'C Cuts at: 1391 4617 4811 7646 8969 1391 Size: 3226 194 2835 1323 4072 Fragments arranged by size: 4072 3226 2835 1323 194 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4073 4431 4917 7663 7945 928 Size: 971 2174 358 486 2746 282 4633 Fragments arranged by size: 4633 2746 2174 971 486 358 282 BbvCI CC'TCA_GC Cuts at: 5521 6888 8843 5521 Size: 1367 1955 8328 Fragments arranged by size: 8328 1955 1367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 7247 7281 9841 9875 1903 Size: 34 50 34 5226 34 2560 34 3678 Fragments arranged by size: 5226 3678 2560 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2069 6484 9542 10752 2069 Size: 4415 3058 1210 2967 Fragments arranged by size: 4415 3058 2967 1210 BclI T'GATC_A Cuts at: 1548 5338 6094 7291 7516 8775 1548 Size: 3790 756 1197 225 1259 4423 Fragments arranged by size: 4423 3790 1259 1197 756 225 BfrBI ATG'CAT Cuts at: 4756 4756 Size: 11650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 10261 136 Size: 107 122 71 631 605 8589 1525 Fragments arranged by size: 8589 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2209 3644 4123 11645 2209 Size: 1435 479 7522 2214 Fragments arranged by size: 7522 2214 1435 479 BlpI GC'TnA_GC Cuts at: 9067 9067 Size: 11650 Bme1580I G_kGCm'C Cuts at: 1790 4456 4569 5613 9711 10867 1790 Size: 2666 113 1044 4098 1156 2573 Fragments arranged by size: 4098 2666 2573 1156 1044 113 BmgBI CAC'GTC Cuts at: 1318 7670 1318 Size: 6352 5298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2307 2401 4088 Size: 416 53 83 399 24 890 94 1687 Cuts at: 4088 4401 5607 7656 9381 10339 442 Size: 313 1206 2049 1725 958 1753 Fragments arranged by size: 2049 1753 1725 1687 1206 958 890 416 399 313 94 83 53 24 BmtI G_CTAG'C Cuts at: 1056 7313 1056 Size: 6257 5393 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3754 3768 4010 4183 5016 7009 7459 7830 Size: 2515 14 242 173 833 1993 450 371 Cuts at: 7830 8344 10310 1239 Size: 514 1966 2579 Fragments arranged by size: 2579 2515 1993 1966 833 514 450 371 242 173 14 Bpu10I CC'TnA_GC Cuts at: 5521 6888 8093 8843 5521 Size: 1367 1205 750 8328 Fragments arranged by size: 8328 1367 1205 750 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2477 4707 5385 9775 10640 10938 2477 Size: 2230 678 4390 865 298 3189 Fragments arranged by size: 4390 3189 2230 865 678 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4222 5510 10313 882 Size: 617 2723 1288 4803 2219 Fragments arranged by size: 4803 2723 2219 1288 617 BsaAI yAC'GTr Cuts at: 493 1204 4049 9441 493 Size: 711 2845 5392 2702 Fragments arranged by size: 5392 2845 2702 711 BsaBI GATnn'nnATC Cuts at: 2208 2465 3105 11644 2208 Size: 257 640 8539 2214 Fragments arranged by size: 8539 2214 640 257 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 9457 9839 10474 275 Size: 53 83 186 936 7924 382 635 1451 Fragments arranged by size: 7924 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4255 10083 10481 10755 10902 11313 4255 Size: 5828 398 274 147 411 4592 Fragments arranged by size: 5828 4592 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2701 2731 7498 7528 1178 Size: 30 165 30 1298 30 4767 30 5300 Fragments arranged by size: 5300 4767 1298 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2361 2706 3308 3725 3728 7559 7955 Size: 847 328 345 602 417 3 3831 396 Cuts at: 7955 8030 8697 1186 Size: 75 667 4139 Fragments arranged by size: 4139 3831 847 667 602 417 396 345 328 75 3 BseYI C'CCAG_C Cuts at: 4592 4616 4736 8289 10853 11349 4592 Size: 24 120 3553 2564 496 4893 Fragments arranged by size: 4893 3553 2564 496 120 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2707 5696 6993 7217 8922 1163 Size: 573 971 2989 1297 224 1705 3891 Fragments arranged by size: 3891 2989 1705 1297 971 573 224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9100 9861 10889 1540 Size: 275 186 7099 761 1028 2301 Fragments arranged by size: 7099 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2134 4456 6592 8710 8858 9711 9796 10867 2134 Size: 2322 2136 2118 148 853 85 1071 2917 Fragments arranged by size: 2917 2322 2136 2118 1071 853 148 85 BsmI GAATG_Cn' Cuts at: 2034 5254 9161 9254 2034 Size: 3220 3907 93 4430 Fragments arranged by size: 4430 3907 3220 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4661 4661 Size: 11650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2134 4456 4569 4617 4811 5613 6592 Size: 399 344 2322 113 48 194 802 979 Cuts at: 6592 7646 8710 8858 8969 9711 9796 10867 1391 Size: 1054 1064 148 111 742 85 1071 2174 Fragments arranged by size: 2322 2174 1071 1064 1054 979 802 742 399 344 194 148 113 111 85 48 BspEI T'CCGG_A Cuts at: 11313 11313 Size: 11650 BspHI T'CATG_A Cuts at: 2923 5341 7476 7960 8668 9539 11269 2923 Size: 2418 2135 484 708 871 1730 3304 Fragments arranged by size: 3304 2418 2135 1730 871 708 484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6746 9005 844 Size: 5902 2259 3489 Fragments arranged by size: 5902 3489 2259 BsrBI CCG'CTC Cuts at: 1394 1823 9537 1394 Size: 429 7714 3507 Fragments arranged by size: 7714 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 8147 8278 8906 10145 10319 65 Size: 1183 6899 131 628 1239 174 1396 Fragments arranged by size: 6899 1396 1239 1183 628 174 131 BsrFI r'CCGG_y Cuts at: 1700 1810 4255 6233 10294 10481 1700 Size: 110 2445 1978 4061 187 2869 Fragments arranged by size: 4061 2869 2445 1978 187 110 BsrGI T'GTAC_A Cuts at: 96 5862 96 Size: 5766 5884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11650 BssSI C'ACGA_G Cuts at: 7490 9710 10722 7490 Size: 2220 1012 8418 Fragments arranged by size: 8418 2220 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3785 7319 7763 794 Size: 2991 3534 444 4681 Fragments arranged by size: 4681 3534 2991 444 BstBI TT'CG_AA Cuts at: 10454 10454 Size: 11650 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 11650 BstXI CCAn_nnnn'nTGG Cuts at: 6155 8887 6155 Size: 2732 8918 BstYI r'GATC_y Cuts at: 1972 2209 2641 3644 3937 4123 4341 6744 8980 Size: 237 432 1003 293 186 218 2403 2236 Cuts at: 8980 9347 9732 9749 11201 11287 11299 11645 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2403 2236 1977 1452 1003 432 385 367 346 293 237 218 186 86 17 12 BstZ17I GTA'TAC Cuts at: 4237 4261 4237 Size: 24 11626 BtgI C'CryG_G Cuts at: 513 1065 4221 8748 513 Size: 552 3156 4527 3415 Fragments arranged by size: 4527 3415 3156 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 10911 Fragments arranged by size: 10911 683 56 BtsI GCAGTG_nn' Cuts at: 4747 7703 7785 7798 7810 9166 9960 9980 4747 Size: 2956 82 13 12 1356 794 20 6417 Fragments arranged by size: 6417 2956 1356 794 82 20 13 12 ClaI AT'CG_AT Cuts at: 1554 7267 1554 Size: 5713 5937 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 7473 7508 529 Size: 35 6909 35 4671 Fragments arranged by size: 6909 4671 35 35 DraI TTT'AAA Cuts at: 2368 2752 3504 3618 8954 2368 Size: 384 752 114 5336 5064 Fragments arranged by size: 5336 5064 752 384 114 DraIII CAC_nnn'GTG Cuts at: 3595 5848 7698 3595 Size: 2253 1850 7547 Fragments arranged by size: 7547 2253 1850 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2336 4075 10657 809 Size: 507 1020 1739 6582 1802 Fragments arranged by size: 6582 1802 1739 1020 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5889 8374 8408 Size: 54 1425 50 275 18 4059 2485 34 Cuts at: 8408 8576 9097 9986 11615 8 Size: 168 521 889 1629 43 Fragments arranged by size: 4059 2485 1629 1425 889 521 275 168 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 9097 1537 Size: 275 7285 4090 Fragments arranged by size: 7285 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 2910 3294 4284 5008 7568 8862 9580 Size: 747 810 384 990 724 2560 1294 718 Cuts at: 9580 1353 Size: 3423 Fragments arranged by size: 3423 2560 1294 990 810 747 724 718 384 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 10237 10611 10757 387 Size: 1064 138 8648 374 146 1280 Fragments arranged by size: 8648 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3146 3297 3591 3747 3754 3768 3774 Size: 393 1514 151 294 156 7 14 6 Cuts at: 3774 4010 4183 4468 4527 4647 4752 5016 5559 Size: 236 173 285 59 120 105 264 543 Cuts at: 5559 6468 7009 7179 7449 7459 7830 8344 8484 Size: 909 541 170 270 10 371 514 140 Cuts at: 8484 9713 10310 11097 11365 1239 Size: 1229 597 787 268 1524 Fragments arranged by size: 1524 1514 1229 909 787 597 543 541 514 393 371 294 285 270 268 264 236 173 170 156 151 140 120 105 59 14 10 7 6 EcoO109I rG'GnC_Cy Cuts at: 4109 4772 5307 7784 4109 Size: 663 535 2477 7975 Fragments arranged by size: 7975 2477 663 535 EcoRI G'AATT_C Cuts at: 3467 6442 8276 8959 9337 3467 Size: 2975 1834 683 378 5780 Fragments arranged by size: 5780 2975 1834 683 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4585 4585 Size: 11650 FspI TGC'GCA Cuts at: 10156 10156 Size: 11650 HaeII r_GCGC'y Cuts at: 1678 3327 5126 7774 10797 1678 Size: 1649 1799 2648 3023 2531 Fragments arranged by size: 3023 2648 2531 1799 1649 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2701 2733 2997 Size: 32 135 32 680 32 417 32 264 Cuts at: 2997 3029 3797 3829 3989 4021 4202 4234 4628 Size: 32 768 32 160 32 181 32 394 Cuts at: 4628 4660 5441 5473 6299 6331 7019 7051 7659 Size: 32 781 32 826 32 688 32 608 Cuts at: 7659 7691 8764 8796 8861 8893 10291 10323 10365 Size: 32 1073 32 65 32 1398 32 42 Cuts at: 10365 10397 1373 Size: 32 2626 Fragments arranged by size: 2626 1398 1073 826 781 768 688 680 608 417 394 264 181 160 135 65 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2737 3223 4228 5578 7213 7882 Size: 988 281 799 486 1005 1350 1635 669 Cuts at: 7882 8994 9240 669 Size: 1112 246 3079 Fragments arranged by size: 3079 1635 1350 1112 1005 988 799 669 486 281 246 HindIII A'AGCT_T Cuts at: 748 3737 5990 7389 748 Size: 2989 2253 1399 5009 Fragments arranged by size: 5009 2989 2253 1399 HpaI GTT'AAC Cuts at: 9240 9240 Size: 11650 KpnI G_GTAC'C Cuts at: 5855 6669 8975 5855 Size: 814 2306 8530 Fragments arranged by size: 8530 2306 814 MfeI C'AATT_G Cuts at: 5958 9249 5958 Size: 3291 8359 MluI A'CGCG_T Cuts at: 11526 11526 Size: 11650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2189 3042 3982 6086 6876 7072 7085 7263 Size: 884 853 940 2104 790 196 13 178 Cuts at: 7263 7795 10764 10948 1305 Size: 532 2969 184 2007 Fragments arranged by size: 2969 2104 2007 940 884 853 790 532 196 184 178 13 MscI TGG'CCA Cuts at: 10 64 1489 1832 8376 8410 8578 10 Size: 54 1425 343 6544 34 168 3082 Fragments arranged by size: 6544 3082 1425 343 168 54 34 MslI CAynn'nnrTG Cuts at: 518 1166 2834 5213 6003 6153 6552 7803 8019 Size: 648 1668 2379 790 150 399 1251 216 Cuts at: 8019 8025 8180 8456 9608 9967 10126 518 Size: 6 155 276 1152 359 159 2042 Fragments arranged by size: 2379 2042 1668 1251 1152 790 648 399 359 276 216 159 155 150 6 MspA1I CmG'CkG Cuts at: 1606 2343 5606 6137 7427 9066 9743 10891 11136 Size: 737 3263 531 1290 1639 677 1148 245 Cuts at: 11136 1606 Size: 2120 Fragments arranged by size: 3263 2120 1639 1290 1148 737 677 531 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 11098 NdeI CA'TA_TG Cuts at: 387 3176 3654 3859 5757 387 Size: 2789 478 205 1898 6280 Fragments arranged by size: 6280 2789 1898 478 205 NheI G'CTAG_C Cuts at: 1052 7309 1052 Size: 6257 5393 NotI GC'GGCC_GC Cuts at: 9097 9097 Size: 11650 NsiI A_TGCA'T Cuts at: 4758 4758 Size: 11650 NspI r_CATG'y Cuts at: 1132 1229 2172 3229 4391 4577 4760 5227 5299 Size: 97 943 1057 1162 186 183 467 72 Cuts at: 5299 5710 5936 6125 7127 7142 9008 10553 1132 Size: 411 226 189 1002 15 1866 1545 2229 Fragments arranged by size: 2229 1866 1545 1162 1057 1002 943 467 411 226 189 186 183 97 72 15 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 11650 PciI A'CATG_T Cuts at: 2168 5223 10549 2168 Size: 3055 5326 3269 Fragments arranged by size: 5326 3269 3055 PflMI CCAn_nnn'nTGG Cuts at: 1108 3061 4592 5848 7780 8356 8383 1108 Size: 1953 1531 1256 1932 576 27 4375 Fragments arranged by size: 4375 1953 1932 1531 1256 576 27 PfoI T'CCnGG_A Cuts at: 6775 8047 6775 Size: 1272 10378 PmeI GTTT'AAAC Cuts at: 8954 8954 Size: 11650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9719 9751 11232 11264 9719 Size: 32 1481 32 10105 Fragments arranged by size: 10105 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4109 5307 4109 Size: 1198 10452 PshAI GACnn'nnGTC Cuts at: 1613 6504 1613 Size: 4891 6759 PsiI TTA'TAA Cuts at: 4704 8501 8618 8627 9220 4704 Size: 3797 117 9 593 7134 Fragments arranged by size: 7134 3797 593 117 9 PspXI vC'TCGA_Gb Cuts at: 3009 3009 Size: 11650 PstI C_TGCA'G Cuts at: 830 3676 4090 4959 7805 8131 9002 830 Size: 2846 414 869 2846 326 871 3478 Fragments arranged by size: 3478 2846 2846 871 869 414 326 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11650 PvuII CAG'CTG Cuts at: 5606 6137 7427 5606 Size: 531 1290 9829 Fragments arranged by size: 9829 1290 531 SalI G'TCGA_C Cuts at: 1936 3221 8992 1936 Size: 1285 5771 4594 Fragments arranged by size: 5771 4594 1285 SanDI GG'GwC_CC Cuts at: 4109 5307 4109 Size: 1198 10452 SbfI CC_TGCA'GG Cuts at: 9002 9002 Size: 11650 ScaI AGT'ACT Cuts at: 5536 7523 7724 9898 11610 5536 Size: 1987 201 2174 1712 5576 Fragments arranged by size: 5576 2174 1987 1712 201 SexAI A'CCwGG_T Cuts at: 8760 8760 Size: 11650 SfcI C'TryA_G Cuts at: 826 1045 2080 3672 3975 4086 4291 4955 5073 Size: 219 1035 1592 303 111 205 664 118 Cuts at: 5073 6108 6346 7801 8127 8998 10133 10814 11005 Size: 1035 238 1455 326 871 1135 681 191 Cuts at: 11005 826 Size: 1471 Fragments arranged by size: 1592 1471 1455 1135 1035 1035 871 681 664 326 303 238 219 205 191 118 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11650 SmaI CCC'GGG Cuts at: 1872 8977 1872 Size: 7105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 2456 3009 4722 5400 9754 Size: 197 926 291 222 553 1713 678 4354 Cuts at: 9754 10655 10917 820 Size: 901 262 1553 Fragments arranged by size: 4354 1713 1553 926 901 678 553 291 262 222 197 SnaBI TAC'GTA Cuts at: 493 9441 493 Size: 8948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11650 SphI G_CATG'C Cuts at: 1132 4391 4577 4760 9008 1132 Size: 3259 186 183 4248 3774 Fragments arranged by size: 4248 3774 3259 186 183 SspI AAT'ATT Cuts at: 5 52 2200 5625 9574 5 Size: 47 2148 3425 3949 2081 Fragments arranged by size: 3949 3425 2148 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 11650 StyI C'CwwG_G Cuts at: 513 1065 3817 5289 7405 9089 513 Size: 552 2752 1472 2116 1684 3074 Fragments arranged by size: 3074 2752 2116 1684 1472 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2502 3107 4750 4856 6233 6401 7260 Size: 1255 922 605 1643 106 1377 168 859 Cuts at: 7260 9677 9694 9847 10032 10477 325 Size: 2417 17 153 185 445 1498 Fragments arranged by size: 2417 1643 1498 1377 1255 922 859 605 445 185 168 153 106 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2871 5534 5862 Size: 274 80 33 51 1443 894 2663 328 Cuts at: 5862 7031 7205 7521 7722 8542 9896 11608 96 Size: 1169 174 316 201 820 1354 1712 138 Fragments arranged by size: 2663 1712 1443 1354 1169 894 820 328 316 274 201 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7498 7530 11610 11642 7498 Size: 32 4080 32 7506 Fragments arranged by size: 7506 4080 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11650 XbaI T'CTAG_A Cuts at: 2882 8986 2882 Size: 6104 5546 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3062 4228 7669 7805 3062 Size: 1166 3441 136 6907 Fragments arranged by size: 6907 3441 1166 136 XhoI C'TCGA_G Cuts at: 1943 3009 1943 Size: 1066 10584 XmaI C'CCGG_G Cuts at: 1870 8975 1870 Size: 7105 4545 XmnI GAAnn'nnTTC Cuts at: 2919 2955 3892 4305 4497 6998 8081 8167 9779 Size: 36 937 413 192 2501 1083 86 1612 Cuts at: 9779 2919 Size: 4790 Fragments arranged by size: 4790 2501 1612 1083 937 413 192 86 36 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9458 276 Size: 53 83 186 936 7924 2468 Fragments arranged by size: 7924 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI