(Circular) (Six-base) MAPSORT of: pFN21AE1612.seq Check: 2039 from: 1 to: 5062 pFN21AE1612 5062 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2366 2366 Size: 5062 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2872 278 Size: 53 83 186 936 1336 2468 Fragments arranged by size: 2468 1336 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2405 1937 Size: 468 4594 Acc65I G'GTAC_C Cuts at: 2383 2383 Size: 5062 AclI AA'CG_TT Cuts at: 1563 3189 3562 1563 Size: 1626 373 3063 Fragments arranged by size: 3063 1626 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3125 4509 4777 1632 Size: 1493 1384 268 1917 Fragments arranged by size: 1917 1493 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4865 AflIII A'CryG_T Cuts at: 1203 3961 4938 1203 Size: 2758 977 1327 Fragments arranged by size: 2758 1327 977 AgeI A'CCGG_T Cuts at: 3893 3893 Size: 5062 AhdI GACnn_n'nnGTC Cuts at: 3791 3791 Size: 5062 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5062 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5062 AlwNI CAG_nnn'CTG Cuts at: 2315 2805 4377 2315 Size: 490 1572 3000 Fragments arranged by size: 3000 1572 490 ApaI G_GGCC'C Cuts at: 2327 2327 Size: 5062 ApaLI G'TGCA_C Cuts at: 3119 4275 3119 Size: 1156 3906 ApoI r'AATT_y Cuts at: 1493 2127 2371 2597 2749 4858 1493 Size: 634 244 226 152 2109 1697 Fragments arranged by size: 2109 1697 634 244 226 152 AseI AT'TA_AT Cuts at: 160 1032 3616 160 Size: 872 2584 1606 Fragments arranged by size: 2584 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5062 AvaI C'yCGr_G Cuts at: 1870 1943 2387 1870 Size: 73 444 4545 Fragments arranged by size: 4545 444 73 AvrII C'CTAG_G Cuts at: 2297 2297 Size: 5062 BamHI G'GATC_C Cuts at: 2392 2759 2392 Size: 367 4695 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2383 3838 618 Size: 325 217 627 596 1455 1842 Fragments arranged by size: 1842 1455 627 596 325 217 BanII G_rGCy'C Cuts at: 1391 2081 2327 2381 1391 Size: 690 246 54 4072 Fragments arranged by size: 4072 690 246 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4091 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3253 3287 1903 Size: 34 1316 34 3678 Fragments arranged by size: 3678 1316 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2954 4164 2954 Size: 1210 3852 BclI T'GATC_A Cuts at: 1548 1548 Size: 5062 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3673 136 Size: 107 122 71 631 605 2001 1525 Fragments arranged by size: 2001 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5057 5057 Size: 5062 BlpI GC'TnA_GC Cuts at: 2479 2479 Size: 5062 Bme1580I G_kGCm'C Cuts at: 1790 2059 2327 3123 4279 1790 Size: 269 268 796 1156 2573 Fragments arranged by size: 2573 1156 796 269 268 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5062 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2793 3751 442 Size: 416 53 83 399 24 1376 958 1753 Fragments arranged by size: 1753 1376 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5062 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3722 1239 Size: 2483 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2165 3187 4052 4350 2165 Size: 1022 865 298 2877 Fragments arranged by size: 2877 1022 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3725 882 Size: 617 2226 2219 Fragments arranged by size: 2226 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2853 493 Size: 711 1649 2702 Fragments arranged by size: 2702 1649 711 BsaBI GATnn'nnATC Cuts at: 5056 5056 Size: 5062 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2274 2869 3251 3886 Size: 53 83 186 936 741 595 382 635 Cuts at: 3886 275 Size: 1451 Fragments arranged by size: 1451 936 741 635 595 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3495 3893 4167 4314 4725 3495 Size: 398 274 147 411 3832 Fragments arranged by size: 3832 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4837 Fragments arranged by size: 4837 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5062 BseYI C'CCAG_C Cuts at: 4265 4761 4265 Size: 496 4566 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2512 3273 4301 1540 Size: 275 186 511 761 1028 2301 Fragments arranged by size: 2301 1028 761 511 275 186 BsiHKAI G_wGCw'C Cuts at: 2174 3123 3208 4279 2174 Size: 949 85 1071 2957 Fragments arranged by size: 2957 1071 949 85 BsmI GAATG_Cn' Cuts at: 2573 2666 2573 Size: 93 4969 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2081 2174 2327 2381 3123 3208 Size: 399 269 22 93 153 54 742 85 Cuts at: 3208 4279 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 269 153 93 85 54 22 BspEI T'CCGG_A Cuts at: 4725 4725 Size: 5062 BspHI T'CATG_A Cuts at: 2951 4681 2951 Size: 1730 3332 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2366 2417 844 Size: 1522 51 3489 Fragments arranged by size: 3489 1522 51 BsrBI CCG'CTC Cuts at: 1394 1823 2949 1394 Size: 429 1126 3507 Fragments arranged by size: 3507 1126 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3557 3731 65 Size: 1183 2309 174 1396 Fragments arranged by size: 2309 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3706 3893 1700 Size: 110 1896 187 2869 Fragments arranged by size: 2869 1896 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5062 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5062 BssSI C'ACGA_G Cuts at: 3122 4134 3122 Size: 1012 4050 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5062 BstBI TT'CG_AA Cuts at: 3866 3866 Size: 5062 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5062 BstXI CCAn_nnnn'nTGG Cuts at: 2311 2311 Size: 5062 BstYI r'GATC_y Cuts at: 1972 2392 2759 3144 3161 4613 4699 4711 5057 Size: 420 367 385 17 1452 86 12 346 Cuts at: 5057 1972 Size: 1977 Fragments arranged by size: 1977 1452 420 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3571 Fragments arranged by size: 3571 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4323 Fragments arranged by size: 4323 683 56 BtsI GCAGTG_nn' Cuts at: 2316 2578 3372 3392 2316 Size: 262 794 20 3986 Fragments arranged by size: 3986 794 262 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5062 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5027 DraI TTT'AAA Cuts at: 2366 2366 Size: 5062 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4069 809 Size: 507 2753 1802 Fragments arranged by size: 2753 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2509 3398 5027 Size: 54 1425 50 275 18 679 889 1629 Cuts at: 5027 8 Size: 43 Fragments arranged by size: 1629 1425 889 679 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2509 1537 Size: 275 697 4090 Fragments arranged by size: 4090 697 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2992 1353 Size: 1639 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3649 4023 4169 387 Size: 1064 138 2060 374 146 1280 Fragments arranged by size: 2060 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3125 3722 4509 4777 1239 Size: 393 1493 597 787 268 1524 Fragments arranged by size: 1524 1493 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2323 2324 2323 Size: 1 5061 EcoRI G'AATT_C Cuts at: 2127 2371 2749 2127 Size: 244 378 4440 Fragments arranged by size: 4440 378 244 EcoRV GAT'ATC Cuts at: 2339 2339 Size: 5062 FspI TGC'GCA Cuts at: 3568 3568 Size: 5062 HaeII r_GCGC'y Cuts at: 1678 4209 1678 Size: 2531 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3703 3735 3777 3809 1373 Size: 32 135 32 2131 32 42 32 2626 Fragments arranged by size: 2626 2131 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2406 2652 669 Size: 988 281 468 246 3079 Fragments arranged by size: 3079 988 468 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5062 HpaI GTT'AAC Cuts at: 2652 2652 Size: 5062 KpnI G_GTAC'C Cuts at: 2387 2387 Size: 5062 MfeI C'AATT_G Cuts at: 2661 2661 Size: 5062 MluI A'CGCG_T Cuts at: 4938 4938 Size: 5062 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4176 4360 1305 Size: 2871 184 2007 Fragments arranged by size: 2871 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3240 Fragments arranged by size: 3240 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3020 3379 3538 518 Size: 648 1854 359 159 2042 Fragments arranged by size: 2042 1854 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2478 3155 4303 4548 1606 Size: 552 320 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 552 320 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3571 Fragments arranged by size: 3571 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5062 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5062 NotI GC'GGCC_GC Cuts at: 2509 2509 Size: 5062 NspI r_CATG'y Cuts at: 1132 1229 2110 2178 2420 3965 1132 Size: 97 881 68 242 1545 2229 Fragments arranged by size: 2229 1545 881 242 97 68 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5062 PciI A'CATG_T Cuts at: 3961 3961 Size: 5062 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5062 PmeI GTTT'AAAC Cuts at: 2366 2366 Size: 5062 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3131 3163 4644 4676 3131 Size: 32 1481 32 3517 Fragments arranged by size: 3517 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5062 PsiI TTA'TAA Cuts at: 2632 2632 Size: 5062 PspOMI G'GGCC_C Cuts at: 2323 2323 Size: 5062 PstI C_TGCA'G Cuts at: 830 2414 830 Size: 1584 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5062 PvuII CAG'CTG Cuts at: 2158 2158 Size: 5062 SalI G'TCGA_C Cuts at: 1936 2404 1936 Size: 468 4594 SbfI CC_TGCA'GG Cuts at: 2414 2414 Size: 5062 ScaI AGT'ACT Cuts at: 3310 5022 3310 Size: 1712 3350 SfcI C'TryA_G Cuts at: 826 1045 2410 3545 4226 4417 826 Size: 219 1365 1135 681 191 1471 Fragments arranged by size: 1471 1365 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5062 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5062 SmaI CCC'GGG Cuts at: 1872 2389 1872 Size: 517 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2180 3166 4067 4329 820 Size: 197 926 237 986 901 262 1553 Fragments arranged by size: 1553 986 926 901 262 237 197 SnaBI TAC'GTA Cuts at: 493 2853 493 Size: 2360 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5062 SphI G_CATG'C Cuts at: 1132 2420 1132 Size: 1288 3774 SspI AAT'ATT Cuts at: 5 52 2986 5 Size: 47 2934 2081 Fragments arranged by size: 2934 2081 47 StuI AGG'CCT Cuts at: 1517 2013 1517 Size: 496 4566 StyI C'CwwG_G Cuts at: 513 1065 2004 2044 2297 2501 513 Size: 552 939 40 253 204 3074 Fragments arranged by size: 3074 939 552 253 204 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3089 3106 3259 3444 3889 325 Size: 1255 1509 17 153 185 445 1498 Fragments arranged by size: 1509 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3308 5020 96 Size: 274 80 33 51 1443 1331 1712 138 Fragments arranged by size: 1712 1443 1331 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5022 5054 5022 Size: 32 5030 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5062 XbaI T'CTAG_A Cuts at: 2398 2398 Size: 5062 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2222 2339 2222 Size: 117 4945 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5062 XmaI C'CCGG_G Cuts at: 1870 2387 1870 Size: 517 4545 XmnI GAAnn'nnTTC Cuts at: 3191 3191 Size: 5062 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2870 276 Size: 53 83 186 936 1336 2468 Fragments arranged by size: 2468 1336 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI