################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DpnII DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HphI ItaI KasI KpnI KspI MamI MboI MfeI MflI MluI MroI MspA1I MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 4 GTAGAC GT^[AC][GT]AC AfaI 168 GTAC GT^AC Asp700I 303 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 55 GAGCAC G[AT]GC[AT]^C BalI 49 TGGCCA TGG^CCA BbvCI 1103 CCTCAGC CCTCAGC(-5/-2) BmyI 55 GAGCAC G[AGT]GC[ACT]^C BsaAI 1370 CACGTA [CT]AC^GT[GA] BsaJI 324 CCGTGG C^C[ACGT][ACGT]GG BsgI 1367 CTGCAC GTGCAG(16/14) BsiHKAI 55 GAGCAC G[AT]GC[AT]^C Bsp1286I 55 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 167 TGTACA T^GTACA BsrDI 1337 GCAATG GCAATG(2/0) BsrGI 167 TGTACA T^GTACA BstF5I 153 GGATG GGATG(2/0) Cac8I 341 GCATGC GC[ACGT]^[ACGT]GC DraI 1009 TTTAAA TTT^AAA DsaI 324 CCGTGG C^C[GA][CT]GG EaeI 49 TGGCCA [CT]^GGCC[GA] EarI 634 GAAGAG CTCTTC(1/4) EcoT22I 155 ATGCAT ATGCA^T FokI 153 GGATG GGATG(9/13) Ksp632I 634 GAAGAG CTCTTC(1/4) MaeII 1371 ACGT A^CGT MaeIII 1418 GTGAC ^GT[ACGT]AC MluNI 49 TGGCCA TGG^CCA MscI 49 TGGCCA TGG^CCA NsiI 155 ATGCAT ATGCA^T RsaI 168 GTAC GT^AC SfaNI 154 GATGC GCATC(5/9) SphI 341 GCATGC GCATG^C SspBI 167 TGTACA T^GTACA TaqI 685 TCGA T^CGA Tsp45I 1418 GTGAC ^GT[GC]AC TthHB8I 685 TCGA T^CGA XmnI 303 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AgeI 509 ACCGGT A^CCGGT 593 ACCGGT BfaI 380 CTAG C^TAG 1220 CTAG BsaWI 509 ACCGGT [AT]^CCGG[AT] 593 ACCGGT BsrFI 509 ACCGGT [GA]^CCGG[CT] 593 ACCGGT Cfr10I 509 ACCGGT [GA]^CCGG[CT] 593 ACCGGT HapII 510 CCGG C^CGG 594 CCGG HinfI 70 GATTC G^A[ACGT]TC 687 GATTC HpaII 510 CCGG C^CGG 594 CCGG MaeI 380 CTAG C^TAG 1220 CTAG MspI 510 CCGG C^CGG 594 CCGG NspI 157 GCATGT [GA]CATG^[CT] 341 GCATGC PinAI 509 ACCGGT A^CCGGT 593 ACCGGT StuI 668 AGGCCT AGG^CCT 1088 AGGCCT TfiI 70 GATTC G^A[AT]TC 687 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AcsI AluI ApoI AvaII BpmI BsiYI BslI BsrI Cfr13I DdeI EcoRI HaeIII HindIII MboII MnlI MseI MslI NdeI NlaIII Sau96I Tru9I Tsp509I TspRI