Restriction map for
pFN21AE6655
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BsrI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HinfI HpaI KpnI Ksp632I KspI
MaeI MaeIII MamI MfeI MluI
MroI MseI MspA1I MunI MvnI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 254 GGCGCC G[GA]^CG[CT]C
AfaI 423 GTAC GT^AC
AflIII 167 ACACGT A^C[GA][CT]GT
AlwNI 691 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 485 GGGCCC GGGCC^C
Asp700I 57 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 245 TGCGCA TGC^GCA
BalI 2 TGGCCA TGG^CCA
BamHI 638 GGATCC G^GATCC
BanI 254 GGCGCC G^G[CT][GA]CC
BanII 485 GGGCCC G[GA]GC[CT]^C
BbeI 254 GGCGCC GGCGC^C
BglII 338 AGATCT A^GATCT
BpmI 342 CTGGAG CTGGAG(16/14)
BsaHI 254 GGCGCC G[GA]^CG[CT]C
BsiEI 308 CGGCCG CG[GA][CT]^CG
BsmFI 100 GGGAC GGGAC(10/14)
BsrBI 265 GAGCGG CCGCTC(-3/-3)
BsrFI 529 GCCGGC [GA]^CCGG[CT]
BssSI 16 CTCGTG CACGAG(-5/-1)
Cfr10I 529 GCCGGC [GA]^CCGG[CT]
DdeI 88 CTGAG C^T[ACGT]AG
EagI 308 CGGCCG C^GGCCG
EclXI 308 CGGCCG C^GGCCG
Eco52I 308 CGGCCG C^GGCCG
EcoNI 291 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 245 TGCGCA TGC^GCA
HgaI 417 GCGTC GACGC(5/10)
Hin1I 254 GGCGCC G[GA]^CG[CT]C
KasI 254 GGCGCC G^GCGCC
MaeII 169 ACGT A^CGT
MboII 336 GAAGA GAAGA(8/7)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 168 CACGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 529 GCCGGC GCC^GGC
NarI 254 GGCGCC GG^CGCC
NcoI 725 CCATGG C^CATGG
NgoMIV 529 GCCGGC G^CCGGC
RsaI 423 GTAC GT^AC
SfiI 280 GGCCTCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 254 GGCGCC GGC^GCC
SmlI 466 CTCAAG C^T[CT][GA]AG
TspRI 173 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 57 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 639 GATCC GGATC(4/5)
638 GGATC
AspHI 39 GAGCAC G[AT]GC[AT]^C
541 GTGCTC
AvaI 439 CCCGGG C^[CT]CG[GA]G
482 CCCGGG
BsiHKAI 39 GAGCAC G[AT]GC[AT]^C
541 GTGCTC
BsoBI 439 CCCGGG C^[CT]CG[GA]G
482 CCCGGG
BsrDI 477 CATTGC GCAATG(2/0)
546 CATTGC
BstYI 338 AGATCT [GA]^GATC[CT]
638 GGATCC
DraII 278 GGGGCCT [GA]G^G[ACGT]CC[CT]
485 GGGCCCT
EaeI 2 TGGCCA [CT]^GGCC[GA]
308 CGGCCG
EcoO109I 278 GGGGCCT [GA]G^G[ACGT]CC[CT]
485 GGGCCCT
EcoT14I 125 CCAAGG C^C[AT][AT]GG
725 CCATGG
HaeII 254 GGCGCC [GA]GCGC^[CT]
347 GGCGCT
MflI 338 AGATCT [GA]^GATC[CT]
638 GGATCC
SfaNI 79 GATGC GCATC(5/9)
139 GATGC
SmaI 439 CCCGGG CCC^GGG
482 CCCGGG
StuI 288 AGGCCT AGG^CCT
429 AGGCCT
StyI 125 CCAAGG C^C[AT][AT]GG
725 CCATGG
TaqI 77 TCGA T^CGA
356 TCGA
TthHB8I 77 TCGA T^CGA
356 TCGA
XcmI 686 CCATGCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
725 CCATGGAGGCTGTGG
XhoII 338 AGATCT [GA]^GATC[CT]
638 GGATCC
XmaCI 439 CCCGGG C^CCGGG
482 CCCGGG
XmaI 439 CCCGGG C^CCGGG
482 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BglI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
DsaI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI