(Circular) (Six-base) MAPSORT of: pFN21AE6655.seq Check: 1508 from: 1 to: 5464 pFN21AE6655 5464 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2326 2468 2326 Size: 142 5322 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 1937 Size: 870 4594 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2204 2459 2696 3527 4911 5179 1632 Size: 572 255 237 831 1384 268 1917 Fragments arranged by size: 1917 1384 831 572 268 255 237 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 2172 4363 5340 1203 Size: 969 2191 977 1327 Fragments arranged by size: 2191 1327 977 969 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 2177 1166 Size: 1011 4453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2561 1184 Size: 1377 4087 AlwNI CAG_nnn'CTG Cuts at: 2701 3207 4779 2701 Size: 506 1572 3386 Fragments arranged by size: 3386 1572 506 ApaI G_GGCC'C Cuts at: 2494 2494 Size: 5464 ApaLI G'TGCA_C Cuts at: 3521 4677 3521 Size: 1156 4308 ApoI r'AATT_y Cuts at: 1493 2773 2999 3151 5260 1493 Size: 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2444 2487 2789 1870 Size: 73 501 43 302 4545 Fragments arranged by size: 4545 501 302 73 43 BamHI G'GATC_C Cuts at: 2643 2794 3161 2643 Size: 151 367 4946 Fragments arranged by size: 4946 367 151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2259 2785 4240 618 Size: 325 217 627 472 526 1455 1842 Fragments arranged by size: 1842 1455 627 526 472 325 217 BanII G_rGCy'C Cuts at: 1391 2494 2783 1391 Size: 1103 289 4072 Fragments arranged by size: 4072 1103 289 BbeI G_GCGC'C Cuts at: 2263 2263 Size: 5464 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4566 3356 Size: 1210 4254 BclI T'GATC_A Cuts at: 1548 1548 Size: 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2292 2577 2735 Size: 107 122 71 631 605 620 285 158 Cuts at: 2735 4075 136 Size: 1340 1525 Fragments arranged by size: 1525 1340 631 620 605 285 158 122 107 71 BglII A'GATC_T Cuts at: 2343 5459 2343 Size: 3116 2348 BlpI GC'TnA_GC Cuts at: 2881 2881 Size: 5464 Bme1580I G_kGCm'C Cuts at: 1790 2494 3525 4681 1790 Size: 704 1031 1156 2573 Fragments arranged by size: 2573 1156 1031 704 BmgBI CAC'GTC Cuts at: 1318 2175 1318 Size: 857 4607 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3195 4153 442 Size: 416 53 83 399 24 1778 958 1753 Fragments arranged by size: 1778 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2368 4124 1239 Size: 1129 1756 2579 Fragments arranged by size: 2579 1756 1129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2456 3589 4454 4752 2456 Size: 1133 865 298 3168 Fragments arranged by size: 3168 1133 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4127 882 Size: 617 2628 2219 Fragments arranged by size: 2628 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3255 493 Size: 711 2051 2702 Fragments arranged by size: 2702 2051 711 BsaBI GATnn'nnATC Cuts at: 5458 5458 Size: 5464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2260 3271 3653 4288 Size: 53 83 186 936 727 1011 382 635 Cuts at: 4288 275 Size: 1451 Fragments arranged by size: 1451 1011 936 727 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2390 2411 2420 2441 1178 Size: 30 165 30 987 21 9 21 4201 Fragments arranged by size: 4201 987 165 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2345 2525 1186 Size: 940 219 180 4125 Fragments arranged by size: 4125 940 219 180 BseYI C'CCAG_C Cuts at: 4667 5163 4667 Size: 496 4968 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4891 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2316 2914 3675 4703 1540 Size: 275 186 315 598 761 1028 2301 Fragments arranged by size: 2301 1028 761 598 315 275 186 BsiHKAI G_wGCw'C Cuts at: 2048 2550 3525 3610 4681 2048 Size: 502 975 85 1071 2831 Fragments arranged by size: 2831 1071 975 502 85 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2494 2550 2783 3525 3610 4681 Size: 399 258 446 56 233 742 85 1071 Cuts at: 4681 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 446 399 258 233 85 56 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 3353 5083 3353 Size: 1730 3734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2326 2468 2640 2819 844 Size: 1482 142 172 179 3489 Fragments arranged by size: 3489 1482 179 172 142 BsrBI CCG'CTC Cuts at: 1394 1823 2272 3351 1394 Size: 429 449 1079 3507 Fragments arranged by size: 3507 1079 449 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2481 2550 3959 4133 65 Size: 1183 1233 69 1409 174 1396 Fragments arranged by size: 1409 1396 1233 1183 174 69 BsrFI r'CCGG_y Cuts at: 1700 1810 2534 4108 4295 1700 Size: 110 724 1574 187 2869 Fragments arranged by size: 2869 1574 724 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 2021 3524 4536 2021 Size: 1503 1012 2949 Fragments arranged by size: 2949 1503 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5464 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstYI r'GATC_y Cuts at: 1972 2343 2643 2794 3161 3546 3563 5015 5101 Size: 371 300 151 367 385 17 1452 86 Cuts at: 5101 5113 5459 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 371 367 346 300 151 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2560 2637 2730 513 Size: 552 939 556 77 93 3247 Fragments arranged by size: 3247 939 556 552 93 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2980 3774 3794 2980 Size: 794 20 4650 Fragments arranged by size: 4650 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4471 809 Size: 507 3155 1802 Fragments arranged by size: 3155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2313 2911 Size: 54 1425 50 275 18 177 306 598 Cuts at: 2911 3800 5429 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 598 306 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2313 2911 1537 Size: 275 501 598 4090 Fragments arranged by size: 4090 598 501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3394 1353 Size: 2041 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4051 4425 4571 387 Size: 1064 138 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2368 2459 2696 3527 4124 4911 Size: 393 572 164 91 237 831 597 787 Cuts at: 4911 5179 1239 Size: 268 1524 Fragments arranged by size: 1524 831 787 597 572 393 268 237 164 91 EcoNI CCTnn'n_nnAGG Cuts at: 2300 2300 Size: 5464 EcoO109I rG'GnC_Cy Cuts at: 2284 2491 2284 Size: 207 5257 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 FspI TGC'GCA Cuts at: 2252 3970 2252 Size: 1718 3746 FspAI rTGC'GCAy Cuts at: 2252 2252 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 2263 2356 4611 1678 Size: 585 93 2255 2531 Fragments arranged by size: 2531 2255 585 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4105 4137 4179 4211 1373 Size: 32 135 32 2533 32 42 32 2626 Fragments arranged by size: 2626 2533 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KasI G'GCGC_C Cuts at: 2259 2259 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 3063 3063 Size: 5464 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4578 4762 1305 Size: 3273 184 2007 Fragments arranged by size: 3273 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3465 Fragments arranged by size: 3465 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 3422 3781 3940 518 Size: 648 1011 1245 359 159 2042 Fragments arranged by size: 2042 1245 1011 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2880 3557 4705 4950 1606 Size: 1274 677 1148 245 2120 Fragments arranged by size: 2120 1274 1148 677 245 NaeI GCC'GGC Cuts at: 2536 2536 Size: 5464 NarI GG'CG_CC Cuts at: 2260 2260 Size: 5464 NcoI C'CATG_G Cuts at: 513 1065 2004 2730 513 Size: 552 939 726 3247 Fragments arranged by size: 3247 939 726 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NgoMIV G'CCGG_C Cuts at: 2534 2534 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2822 4367 1132 Size: 97 1593 1545 2229 Fragments arranged by size: 2229 1593 1545 97 PasI CC'CwG_GG Cuts at: 1348 2749 1348 Size: 1401 4063 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PfoI T'CCnGG_A Cuts at: 2214 2214 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 3034 3034 Size: 5464 PspOMI G'GGCC_C Cuts at: 2490 2490 Size: 5464 PstI C_TGCA'G Cuts at: 830 2816 830 Size: 1986 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 3712 5424 3712 Size: 1712 3752 SfcI C'TryA_G Cuts at: 826 1045 2812 3947 4628 4819 826 Size: 219 1767 1135 681 191 1471 Fragments arranged by size: 1767 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2292 2292 Size: 5464 SfoI GGC'GCC Cuts at: 2261 2261 Size: 5464 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2446 2489 2791 1872 Size: 574 43 302 4545 Fragments arranged by size: 4545 574 302 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 2471 3568 4469 4731 820 Size: 197 926 528 1097 901 262 1553 Fragments arranged by size: 1553 1097 926 901 528 262 197 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SrfI GCCC'GGGC Cuts at: 2489 2489 Size: 5464 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 2295 2436 1517 Size: 778 141 4545 Fragments arranged by size: 4545 778 141 StyI C'CwwG_G Cuts at: 513 1065 2004 2130 2730 2760 2903 513 Size: 552 939 126 600 30 143 3074 Fragments arranged by size: 3074 939 600 552 143 126 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 2693 3491 3508 3661 3846 4291 Size: 1255 543 570 798 17 153 185 445 Cuts at: 4291 325 Size: 1498 Fragments arranged by size: 1498 1255 798 570 543 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3710 5422 96 Size: 274 80 33 51 1443 1733 1712 138 Fragments arranged by size: 1733 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2219 2251 5424 5456 2219 Size: 32 3173 32 2227 Fragments arranged by size: 3173 2227 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2698 2737 2698 Size: 39 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2444 2487 2789 1870 Size: 574 43 302 4545 Fragments arranged by size: 4545 574 302 43 XmnI GAAnn'nnTTC Cuts at: 2066 3593 2066 Size: 1527 3937 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SwaI