Restriction map for
pFN21AE3378
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AviII AvrII BalI BanII
BbeI BbrPI BbvCI BcnI BfrI
BglI BglII BlnI BmyI BsaBI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstXI BstZ17I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I FseI FspI
Hin1I KasI KpnI KspI MamI
MluI MluNI MroI MscI NarI
NciI NcoI NdeI NheI NotI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SpeI SphI Sse8387I SspBI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 1970 GTCTAC GT^[AC][GT]AC
AccII 3373 CGCG CG^CG
AflIII 1935 ACATGT A^C[GA][CT]GT
Aor51HI 824 AGCGCT AGC^GCT
AvaI 1332 CCCGAG C^[CT]CG[GA]G
BamHI 2640 GGATCC G^GATCC
BanI 1230 GGTGCC G^G[CT][GA]CC
BbsI 635 GAAGAC GAAGAC(2/6)
BcgI 234 GCAGGAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3342 TGATCA T^GATCA
BlpI 148 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 148 GCTGAGC GC^T[ACGT]AGC
BpuAI 635 GAAGAC GAAGAC(2/6)
BsaAI 847 TACGTA [CT]AC^GT[GA]
BsaI 1029 GAGACC GGTCTC(1/5)
BsoBI 1332 CCCGAG C^[CT]CG[GA]G
BspHI 1067 TCATGA T^CATGA
BspLU11I 1935 ACATGT A^CATGT
BsrBI 2683 GAGCGG CCGCTC(-3/-3)
BsrFI 80 GCCGGC [GA]^CCGG[CT]
BstAPI 457 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2593 GGTGACC G^GT[ACGT]ACC
BstPI 2593 GGTGACC G^GT[ACGT]ACC
BstUI 3373 CGCG CG^CG
CelII 148 GCTGAGC GC^T[ACGT]AGC
CfoI 825 GCGC GCG^C
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
DraII 2970 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 2602 CCACGG C^C[GA][CT]GG
Eco47III 824 AGCGCT AGC^GCT
EcoO109I 2970 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2593 GGTGACC G^GT[ACGT]ACC
EcoRI 406 GAATTC G^AATTC
EcoRV 2263 GATATC GAT^ATC
EcoT14I 1763 CCAAGG C^C[AT][AT]GG
EcoT22I 3218 ATGCAT ATGCA^T
FbaI 3342 TGATCA T^GATCA
HaeII 824 AGCGCT [GA]GCGC^[CT]
HapII 81 CCGG C^CGG
HhaI 825 GCGC GCG^C
HinP1I 825 GCGC G^CGC
HpaI 514 GTTAAC GTT^AAC
HpaII 81 CCGG C^CGG
MaeII 848 ACGT A^CGT
MspA1I 461 CAGCTG C[AC]G^C[GT]G
MspI 81 CCGG C^CGG
MvnI 3373 CGCG CG^CG
NaeI 80 GCCGGC GCC^GGC
NgoMIV 80 GCCGGC G^CCGGC
NruI 3372 TCGCGA TCG^CGA
NsiI 3218 ATGCAT ATGCA^T
PpuMI 2970 AGGACCC [GA]G^G[AT]CC[CT]
PstI 455 CTGCAG CTGCA^G
PvuII 461 CAGCTG CAG^CTG
RcaI 1067 TCATGA T^CATGA
SnaBI 847 TACGTA TAC^GTA
SspI 2497 AATATT AAT^ATT
StuI 40 AGGCCT AGG^CCT
StyI 1763 CCAAGG C^C[AT][AT]GG
SwaI 1914 ATTTAAAT ATTT^AAAT
XcmI 874 CCACCTGGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 458 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1454 CAGTCACTG
AocI 111 CCTTAGG CC^T[ACGT]AGG
1597 CCTGAGG
Asp700I 1604 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2449 GAAAGTGTTC
AvaII 1660 GGACC G^G[AT]CC
2971 GGACC
BciVI 1556 GGATAC GTATCC(6/5)
2776 GGATAC
BpmI 1316 CTCCAG CTGGAG(16/14)
3045 CTCCAG
BsmI 1037 GAATGC GAATGC(1/-1)
3220 GCATTC
Bsu36I 111 CCTTAGG CC^T[ACGT]AGG
1597 CCTGAGG
DraI 1915 TTTAAA TTT^AAA
3028 TTTAAA
Eco81I 111 CCTTAGG CC^T[ACGT]AGG
1597 CCTGAGG
FauI 573 CCCGC CCCGC(4/6)
2007 GCGGG
HgaI 216 GACGC GACGC(5/10)
1968 GCGTC
HincII 171 GTCAAC GT[CT]^[GA]AC
514 GTTAAC
HindII 171 GTCAAC GT[CT]^[GA]AC
514 GTTAAC
HindIII 1755 AAGCTT A^AGCTT
2939 AAGCTT
MfeI 401 CAATTG C^AATTG
3035 CAATTG
MunI 401 CAATTG C^AATTG
3035 CAATTG
NspI 1935 ACATGT [GA]CATG^[CT]
3216 ACATGC
PshAI 638 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3059 GACAGCAGTC
SapI 1870 GAAGAGC GCTCTTC(1/4)
364 GCTCTTC
ScaI 289 AGTACT AGT^ACT
2579 AGTACT
SfcI 455 CTGCAG C^T[GA][CT]AG
1522 CTACAG
XmnI 1604 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2449 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI BbvI BfaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstBI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoNI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfuI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII