(Circular) (Six-base) MAPSORT of: pFN21AE3378.seq Check: 5955 from: 1 to: 8095 pFN21AE3378 8095 bp With 182 enzymes: SgfI * March 28, 2012 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5905 278 Size: 53 83 186 936 4369 2468 Fragments arranged by size: 4369 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3976 5438 1937 Size: 2039 1462 4594 Fragments arranged by size: 4594 2039 1462 Acc65I G'GTAC_C Cuts at: 5416 5416 Size: 8095 AclI AA'CG_TT Cuts at: 1563 6222 6595 1563 Size: 4659 373 3063 Fragments arranged by size: 4659 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2283 2357 3666 4299 4546 4697 4782 4962 Size: 651 74 1309 633 247 151 85 180 Cuts at: 4962 6158 7542 7810 1632 Size: 1196 1384 268 1917 Fragments arranged by size: 1917 1384 1309 1196 651 633 268 247 180 151 85 74 AfeI AGC'GCT Cuts at: 2831 2831 Size: 8095 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7898 AflIII A'CryG_T Cuts at: 1203 3940 6994 7971 1203 Size: 2737 3054 977 1327 Fragments arranged by size: 3054 2737 1327 977 AgeI A'CCGG_T Cuts at: 6926 6926 Size: 8095 AhdI GACnn_n'nnGTC Cuts at: 6824 6824 Size: 8095 AleI CACnn'nnGTG Cuts at: 1166 4792 1166 Size: 3626 4469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2475 1184 Size: 1291 6804 AlwNI CAG_nnn'CTG Cuts at: 2468 3464 5838 7410 2468 Size: 996 2374 1572 3153 Fragments arranged by size: 3153 2374 1572 996 ApaLI G'TGCA_C Cuts at: 6152 7308 6152 Size: 1156 6939 ApoI r'AATT_y Cuts at: 1493 2103 2122 2213 2244 2281 2411 3014 3391 Size: 610 19 91 31 37 130 603 377 Cuts at: 3391 3923 5116 5234 5404 5630 5782 7891 1493 Size: 532 1193 118 170 226 152 2109 1697 Fragments arranged by size: 2109 1697 1193 610 603 532 377 226 170 152 130 118 91 37 31 19 AseI AT'TA_AT Cuts at: 160 1032 2481 4385 5310 6649 160 Size: 872 1449 1904 925 1339 1606 Fragments arranged by size: 1904 1606 1449 1339 925 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8095 AvaI C'yCGr_G Cuts at: 1870 1943 3337 5420 1870 Size: 73 1394 2083 4545 Fragments arranged by size: 4545 2083 1394 73 BamHI G'GATC_C Cuts at: 4645 5425 5792 4645 Size: 780 367 6948 Fragments arranged by size: 6948 780 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3235 5416 6871 618 Size: 325 217 627 1448 2181 1455 1842 Fragments arranged by size: 2181 1842 1455 1448 627 325 217 BanII G_rGCy'C Cuts at: 1391 5414 1391 Size: 4023 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2647 928 Size: 971 748 6376 Fragments arranged by size: 6376 971 748 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2228 2262 6286 6320 1903 Size: 34 291 34 4024 34 3678 Fragments arranged by size: 4024 3678 291 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3555 4775 5987 7197 3555 Size: 1220 1212 1210 4453 Fragments arranged by size: 4453 1220 1212 1210 BclI T'GATC_A Cuts at: 1548 5347 1548 Size: 3799 4296 BfrBI ATG'CAT Cuts at: 5225 5225 Size: 8095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6706 136 Size: 107 122 71 631 605 5034 1525 Fragments arranged by size: 5034 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8090 8090 Size: 8095 BlpI GC'TnA_GC Cuts at: 2154 5512 2154 Size: 3358 4737 Bme1580I G_kGCm'C Cuts at: 1790 6156 7312 1790 Size: 4366 1156 2573 Fragments arranged by size: 4366 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8095 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3322 3585 5826 Size: 416 53 83 399 24 1905 263 2241 Cuts at: 5826 6784 442 Size: 958 1753 Fragments arranged by size: 2241 1905 1753 958 416 399 263 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8095 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2757 2757 Size: 8095 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3306 5035 6755 1239 Size: 2067 1729 1720 2579 Fragments arranged by size: 2579 2067 1729 1720 Bpu10I CC'TnA_GC Cuts at: 2148 4990 2148 Size: 2842 5253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2709 3897 4011 4636 6220 7085 7383 2709 Size: 1188 114 625 1584 865 298 3421 Fragments arranged by size: 3421 1584 1188 865 625 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3028 6758 882 Size: 617 1529 3730 2219 Fragments arranged by size: 3730 2219 1529 617 BsaAI yAC'GTr Cuts at: 493 1204 2854 5886 493 Size: 711 1650 3032 2702 Fragments arranged by size: 3032 2702 1650 711 BsaBI GATnn'nnATC Cuts at: 8089 8089 Size: 8095 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5902 6284 6919 275 Size: 53 83 186 936 4369 382 635 1451 Fragments arranged by size: 4369 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6528 6926 7200 7347 7758 6528 Size: 398 274 147 411 6865 Fragments arranged by size: 6865 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2580 2610 2758 2779 2788 Size: 30 165 30 1177 30 148 21 9 Cuts at: 2788 2809 2843 2873 3463 3493 4798 4828 1178 Size: 21 34 30 590 30 1305 30 4445 Fragments arranged by size: 4445 1305 1177 590 165 148 34 30 30 30 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2733 3900 1186 Size: 840 707 1167 5381 Fragments arranged by size: 5381 1167 840 707 BseYI C'CCAG_C Cuts at: 7298 7794 7298 Size: 496 7599 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2528 3680 4467 4537 4683 1163 Size: 573 792 1152 787 70 146 4575 Fragments arranged by size: 4575 1152 792 787 573 146 70 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5545 6306 7334 1540 Size: 275 186 3544 761 1028 2301 Fragments arranged by size: 3544 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6156 6241 7312 6156 Size: 85 1071 6939 Fragments arranged by size: 6939 1071 85 BsmI GAATG_Cn' Cuts at: 3048 5225 5606 5699 3048 Size: 2177 381 93 5444 Fragments arranged by size: 5444 2177 381 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 5414 6156 6241 7312 1391 Size: 399 3624 742 85 1071 2174 Fragments arranged by size: 3624 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7758 7758 Size: 8095 BspHI T'CATG_A Cuts at: 3072 5984 7714 3072 Size: 2912 1730 3453 Fragments arranged by size: 3453 2912 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5450 844 Size: 4606 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4690 5982 1394 Size: 429 2867 1292 3507 Fragments arranged by size: 3507 2867 1292 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6590 6764 65 Size: 1183 5342 174 1396 Fragments arranged by size: 5342 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 6739 6926 1700 Size: 110 275 4654 187 2869 Fragments arranged by size: 4654 2869 275 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8095 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8095 BssSI C'ACGA_G Cuts at: 6155 7167 6155 Size: 1012 7083 BstAPI GCAn_nnn'nTGC Cuts at: 794 2468 794 Size: 1674 6421 BstBI TT'CG_AA Cuts at: 2248 4213 4234 6899 2248 Size: 1965 21 2665 3444 Fragments arranged by size: 3444 2665 1965 21 BstEII G'GTnAC_C Cuts at: 1184 4598 1184 Size: 3414 4681 BstYI r'GATC_y Cuts at: 1972 2185 2437 2654 3182 4645 5425 5792 6177 Size: 213 252 217 528 1463 780 367 385 Cuts at: 6177 6194 7646 7732 7744 8090 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1463 1452 780 528 385 367 346 252 217 213 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2117 3603 2117 Size: 1486 6609 BtgI C'CryG_G Cuts at: 513 1065 4607 513 Size: 552 3542 4001 Fragments arranged by size: 4001 3542 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4729 487 Size: 683 56 3503 3853 Fragments arranged by size: 3853 3503 683 56 BtsI GCAGTG_nn' Cuts at: 3349 3462 5611 6405 6425 3349 Size: 113 2149 794 20 5019 Fragments arranged by size: 5019 2149 794 113 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8095 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8060 DraI TTT'AAA Cuts at: 3922 5035 5399 3922 Size: 1113 364 6618 Fragments arranged by size: 6618 1113 364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7102 809 Size: 507 5786 1802 Fragments arranged by size: 5786 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5542 6431 8060 Size: 54 1425 50 275 18 3712 889 1629 Cuts at: 8060 8 Size: 43 Fragments arranged by size: 3712 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5542 1537 Size: 275 3730 4090 Fragments arranged by size: 4090 3730 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2003 2376 3870 4215 4914 6025 1353 Size: 650 373 1494 345 699 1111 3423 Fragments arranged by size: 3423 1494 1111 699 650 373 345 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6682 7056 7202 387 Size: 1064 138 5093 374 146 1280 Fragments arranged by size: 5093 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2357 3306 3666 4299 4546 4697 Size: 393 651 74 949 360 633 247 151 Cuts at: 4697 4782 4962 5035 6158 6755 7542 7810 1239 Size: 85 180 73 1123 597 787 268 1524 Fragments arranged by size: 1524 1123 949 787 651 633 597 393 360 268 247 180 151 85 74 73 EcoNI CCTnn'n_nnAGG Cuts at: 2757 2778 2946 5245 2757 Size: 21 168 2299 5607 Fragments arranged by size: 5607 2299 168 21 EcoO109I rG'GnC_Cy Cuts at: 4976 4976 Size: 8095 EcoRI G'AATT_C Cuts at: 2411 5404 5782 2411 Size: 2993 378 4724 Fragments arranged by size: 4724 2993 378 EcoRV GAT'ATC Cuts at: 4270 4270 Size: 8095 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4244 4244 Size: 8095 FspI TGC'GCA Cuts at: 6601 6601 Size: 8095 HaeII r_GCGC'y Cuts at: 1678 2833 7242 1678 Size: 1155 4409 2531 Fragments arranged by size: 4409 2531 1155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2758 2790 2976 3008 5318 Size: 32 135 32 1186 32 186 32 2310 Cuts at: 5318 5350 5359 5391 6736 6768 6810 6842 1373 Size: 32 9 32 1345 32 42 32 2626 Fragments arranged by size: 2626 2310 1345 1186 186 135 42 32 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 2178 2521 5439 5685 669 Size: 988 281 240 343 2918 246 3079 Fragments arranged by size: 3079 2918 988 343 281 246 240 HindIII A'AGCT_T Cuts at: 748 3760 4944 748 Size: 3012 1184 3899 Fragments arranged by size: 3899 3012 1184 HpaI GTT'AAC Cuts at: 2521 5685 2521 Size: 3164 4931 KpnI G_GTAC'C Cuts at: 5420 5420 Size: 8095 MfeI C'AATT_G Cuts at: 2406 5040 5694 2406 Size: 2634 654 4807 Fragments arranged by size: 4807 2634 654 MluI A'CGCG_T Cuts at: 7971 7971 Size: 8095 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3997 5316 7209 7393 1305 Size: 2692 1319 1893 184 2007 Fragments arranged by size: 2692 2007 1893 1319 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6273 Fragments arranged by size: 6273 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3084 3380 4792 6053 6412 6571 518 Size: 648 1918 296 1412 1261 359 159 2042 Fragments arranged by size: 2042 1918 1412 1261 648 359 296 159 MspA1I CmG'CkG Cuts at: 1606 2468 5511 6188 7336 7581 1606 Size: 862 3043 677 1148 245 2120 Fragments arranged by size: 3043 2120 1148 862 677 245 NaeI GCC'GGC Cuts at: 2087 2087 Size: 8095 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7543 NdeI CA'TA_TG Cuts at: 387 387 Size: 8095 NgoMIV G'CCGG_C Cuts at: 2085 2085 Size: 8095 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8095 NotI GC'GGCC_GC Cuts at: 5542 5542 Size: 8095 NruI TCG'CGA Cuts at: 5379 5379 Size: 8095 NsiI A_TGCA'T Cuts at: 5227 5227 Size: 8095 NspI r_CATG'y Cuts at: 1132 1229 3944 5225 5453 6998 1132 Size: 97 2715 1281 228 1545 2229 Fragments arranged by size: 2715 2229 1545 1281 228 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8095 PciI A'CATG_T Cuts at: 3940 6994 3940 Size: 3054 5041 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8095 PmeI GTTT'AAAC Cuts at: 5399 5399 Size: 8095 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6164 6196 7677 7709 6164 Size: 32 1481 32 6550 Fragments arranged by size: 6550 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4976 4976 Size: 8095 PshAI GACnn'nnGTC Cuts at: 1613 2647 5068 1613 Size: 1034 2421 4640 Fragments arranged by size: 4640 2421 1034 PsiI TTA'TAA Cuts at: 5665 5665 Size: 8095 PstI C_TGCA'G Cuts at: 830 2464 5447 830 Size: 1634 2983 3478 Fragments arranged by size: 3478 2983 1634 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8095 PvuII CAG'CTG Cuts at: 2468 2468 Size: 8095 SalI G'TCGA_C Cuts at: 1936 5437 1936 Size: 3501 4594 SapI GCTCTTCn'nnn_ Cuts at: 2376 3870 2376 Size: 1494 6601 SbfI CC_TGCA'GG Cuts at: 5447 5447 Size: 8095 ScaI AGT'ACT Cuts at: 2296 4586 6343 8055 2296 Size: 2290 1757 1712 2336 Fragments arranged by size: 2336 2290 1757 1712 SfcI C'TryA_G Cuts at: 826 1045 2460 3527 5443 6578 7259 7450 826 Size: 219 1415 1067 1916 1135 681 191 1471 Fragments arranged by size: 1916 1471 1415 1135 1067 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8095 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8095 SmaI CCC'GGG Cuts at: 1872 5422 1872 Size: 3550 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2724 3912 4026 4651 6199 7100 Size: 197 926 781 1188 114 625 1548 901 Cuts at: 7100 7362 820 Size: 262 1553 Fragments arranged by size: 1553 1548 1188 926 901 781 625 262 197 114 SnaBI TAC'GTA Cuts at: 493 2854 5886 493 Size: 2361 3032 2702 Fragments arranged by size: 3032 2702 2361 SpeI A'CTAG_T Cuts at: 152 152 Size: 8095 SphI G_CATG'C Cuts at: 1132 5453 1132 Size: 4321 3774 SspI AAT'ATT Cuts at: 5 52 4504 6019 5 Size: 47 4452 1515 2081 Fragments arranged by size: 4452 2081 1515 47 StuI AGG'CCT Cuts at: 1517 2047 1517 Size: 530 7565 StyI C'CwwG_G Cuts at: 513 1065 3768 5534 513 Size: 552 2703 1766 3074 Fragments arranged by size: 3074 2703 1766 552 SwaI ATTT'AAAT Cuts at: 3922 3922 Size: 8095 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2224 5115 6122 6139 6292 6477 6922 Size: 1255 644 2891 1007 17 153 185 445 Cuts at: 6922 325 Size: 1498 Fragments arranged by size: 2891 1498 1255 1007 644 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2294 3952 4584 Size: 274 80 33 51 1443 317 1658 632 Cuts at: 4584 6341 8053 96 Size: 1757 1712 138 Fragments arranged by size: 1757 1712 1658 1443 632 317 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2580 2612 2688 2720 4798 4830 8055 8087 2580 Size: 32 76 32 2078 32 3225 32 2588 Fragments arranged by size: 3225 2588 2078 76 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8095 XbaI T'CTAG_A Cuts at: 5431 5431 Size: 8095 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2886 2886 Size: 8095 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8095 XmaI C'CCGG_G Cuts at: 1870 5420 1870 Size: 3550 4545 XmnI GAAnn'nnTTC Cuts at: 3613 4458 6224 3613 Size: 845 1766 5484 Fragments arranged by size: 5484 1766 845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5903 276 Size: 53 83 186 936 4369 2468 Fragments arranged by size: 4369 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NarI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI