##################################
# NO CUT ENZYMES (  220 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FbaI        Fnu4HI      FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MflI        MluI        MroI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TseI        
Tsp45I      Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AccI          280  GTCTAC               GT^[AC][GT]AC
AciI           67  GCGG                 CCGC(-3/-1)
AseI           52  ATTAAT               AT^TAAT
AsnI           52  ATTAAT               AT^TAAT
BalI            2  TGGCCA               TGG^CCA
BsaJI         148  CCACGG               C^C[ACGT][ACGT]GG
BsmFI          69  GGGAC                GGGAC(10/14)
BspMI         216  ACCTGC               ACCTGC(4/8)
BstF5I         75  GGATG                GGATG(2/0)
DpnII         171  GATC                 ^GATC
DsaI          148  CCACGG               C^C[GA][CT]GG
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EcoNI         268  CCTACGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI           67  GCGGG                CCCGC(4/6)
FokI           75  GGATG                GGATG(9/13)
HaeIII          3  GGCC                 GG^CC
HinfI         176  GATTC                G^A[ACGT]TC
MboI          171  GATC                 ^GATC
MfeI          248  CAATTG               C^AATTG
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MunI          248  CAATTG               C^AATTG
NdeII         171  GATC                 ^GATC
PflMI         196  CCAATCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI          52  ATTAAT               AT^TAAT
Sau3AI        171  GATC                 ^GATC
TfiI          176  GATTC                G^A[AT]TC
Van91I        196  CCAATCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BsgI          218  CTGCAC               GTGCAG(16/14)
               88  GTGCAG              
BsiYI         196  CCAATCAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              268  CCTACGGGAGG         
BslI          196  CCAATCAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              268  CCTACGGGAGG         
DdeI           35  CTGAG                C^T[ACGT]AG
              118  CTTAG               
MnlI          105  GAGG                 CCTC(7/6)
              275  GAGG                
Tsp509I        55  AATT                 ^AATT
              249  AATT                
TspRI         161  CAGTG                CA[GC]TG[ACGT][ACGT]^
              201  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

MboII       MseI        Tru9I