(Linear) (Six-base) MAPSORT of: KIEE6525.seq  Check: 7030  from: 1  to: 285



KIEE6525.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AccI GT'mk_AC

Cuts at:      0     281     285 
   Size:        281       4
 
AseI AT'TA_AT

Cuts at:      0      53     285 
   Size:         53     232
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     203     285 
   Size:        109      94      82

  Fragments arranged by size: 

                 109      94      82
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     225     285 
   Size:        225      60
 
BtgI C'CryG_G

Cuts at:      0     148     285 
   Size:        148     137
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     137     172     285 
   Size:        137      35     113

  Fragments arranged by size: 

                 137     113      35
 
EaeI y'GGCC_r

Cuts at:      0       2     285 
   Size:          2     283
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     272     285 
   Size:        272      13
 
MfeI C'AATT_G

Cuts at:      0     248     285 
   Size:        248      37
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     285 
   Size:        206      79
 
MscI TGG'CCA

Cuts at:      0       4     285 
   Size:          4     281
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     202     285 
   Size:        202      83
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     146     178     285 
   Size:        146      32     107

  Fragments arranged by size: 

                 146     107      32
 

 Enzymes that do cut:

     AccI     AseI     BsgI    BspMI     BtgI    CspCI     EaeI    EcoNI
     MfeI     MmeI     MscI    PflMI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI