################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I CpoI DdeI DpnII DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 9 GTCGAC GT^[AC][GT]AC AfaI 223 GTAC GT^AC AvaII 126 GGTCC G^G[AT]CC BbsI 73 GTCTTC GAAGAC(2/6) BbvI 232 GCAGC GCAGC(8/12) BpmI 103 CTGGAG CTGGAG(16/14) BpuAI 73 GTCTTC GAAGAC(2/6) BsaJI 54 CCGTGG C^C[ACGT][ACGT]GG BsmAI 19 GTCTC GTCTC(1/5) BspDI 155 ATCGAT AT^CGAT BsrI 225 ACTGG ACTGG(1/-1) BssKI 173 CCAGG ^CC[ACGT]GG BstNI 173 CCAGG CC^[AT]GG CfoI 4 GCGC GCG^C Cfr13I 126 GGTCC G^G[ACGT]CC ClaI 155 ATCGAT AT^CGAT DrdI 118 GACTTATTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 54 CCGTGG C^C[GA][CT]GG EaeI 227 TGGCCG [CT]^GGCC[GA] EcoRII 173 CCAGG ^CC[AT]GG HaeII 3 GGCGCT [GA]GCGC^[CT] HaeIII 228 GGCC GG^CC HhaI 4 GCGC GCG^C HinP1I 4 GCGC G^CGC HincII 9 GTCGAC GT[CT]^[GA]AC HindII 9 GTCGAC GT[CT]^[GA]AC HphI 115 GGTGA GGTGA(8/7) MaeIII 116 GTGAC ^GT[ACGT]AC MseI 209 TTAA T^TAA MvaI 173 CCAGG CC^[AT]GG NlaIII 220 CATG CATG^ NlaIV 48 GGCTCC GG[ACGT]^[ACGT]CC NspI 219 GCATGT [GA]CATG^[CT] PshAI 12 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 223 GTAC GT^AC SalI 9 GTCGAC G^TCGAC SapI 252 GAAGAGC GCTCTTC(1/4) Sau96I 126 GGTCC G^G[ACGT]CC ScrFI 173 CCAGG CC^[ACGT]GG Tru9I 209 TTAA T^TAA TseI 232 GCAGC G^C[AT]GC Tsp45I 116 GTGAC ^GT[GC]AC Tsp509I 243 AATT ^AATT ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AciI 230 CCGC CCGC(-3/-1) 42 GCGG EarI 166 GAAGAG CTCTTC(1/4) 252 GAAGAG Fnu4HI 229 GCCGC GC^[ACGT]GC 232 GCAGC ItaI 229 GCCGC GC^[ACGT]GC 232 GCAGC Ksp632I 166 GAAGAG CTCTTC(1/4) 252 GAAGAG MwoI 33 GCAGATAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 81 GCGAAAGAAGC TaqI 10 TCGA T^CGA 156 TCGA TthHB8I 10 TCGA T^CGA 156 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### HinfI MboII MnlI PleI