################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeII MluI MroI MspA1I NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 578 CGCG CG^CG AcsI 590 GAATTC [GA]^AATT[CT] AflIII 251 ACATGT A^C[GA][CT]GT ApoI 590 GAATTC [GA]^AATT[CT] AspHI 55 GAGCTC G[AT]GC[AT]^C AvaII 333 GGACC G^G[AT]CC AviII 140 TGCGCA TGC^GCA BalI 323 TGGCCA TGG^CCA BamHI 912 GGATCC G^GATCC BanII 55 GAGCTC G[GA]GC[CT]^C BclI 479 TGATCA T^GATCA BglII 895 AGATCT A^GATCT BmyI 55 GAGCTC G[AGT]GC[ACT]^C BsaBI 896 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 79 CTGCAC GTGCAG(16/14) BsiHKAI 55 GAGCTC G[AT]GC[AT]^C BsmFI 775 GGGAC GGGAC(10/14) BsmI 752 GAATGC GAATGC(1/-1) Bsp1286I 55 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 629 TGTACA T^GTACA BspLU11I 251 ACATGT A^CATGT BspMI 386 GCAGGT ACCTGC(4/8) BsrDI 965 CATTGC GCAATG(2/0) BsrGI 629 TGTACA T^GTACA BstAPI 960 GCAACCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 578 CGCG CG^CG DraII 317 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 610 CCATGG C^C[GA][CT]GG EaeI 323 TGGCCA [CT]^GGCC[GA] EarI 891 GAAGAG CTCTTC(1/4) EcoO109I 317 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 590 GAATTC G^AATTC EcoT14I 610 CCATGG C^C[AT][AT]GG FauI 174 GCGGG CCCGC(4/6) FbaI 479 TGATCA T^GATCA FspI 140 TGCGCA TGC^GCA HindIII 261 AAGCTT A^AGCTT Ksp632I 891 GAAGAG CTCTTC(1/4) MamI 896 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 372 CAATTG C^AATTG MluNI 323 TGGCCA TGG^CCA MscI 323 TGGCCA TGG^CCA MseI 266 TTAA T^TAA MunI 372 CAATTG C^AATTG MvnI 578 CGCG CG^CG NcoI 610 CCATGG C^CATGG PstI 559 CTGCAG CTGCA^G SacI 55 GAGCTC GAGCT^C SbfI 558 CCTGCAGG CCTGCA^GG SphI 845 GCATGC GCATG^C Sse8387I 558 CCTGCAGG CCTGCA^GG SspBI 629 TGTACA T^GTACA StyI 610 CCATGG C^C[AT][AT]GG TfiI 351 GATTC G^A[AT]TC Tru9I 266 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 704 GTCTAC GT^[AC][GT]AC 832 GTAGAC AciI 207 CCGC CCGC(-3/-1) 174 GCGG AlwNI 241 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 292 CAGGCACTG BsaI 31 GAGACC GGTCTC(1/5) 19 GGTCTC BsmAI 31 GAGAC GTCTC(1/5) 20 GTCTC BsrFI 16 GCCGGT [GA]^CCGG[CT] 233 ACCGGC BsrI 396 ACTGG ACTGG(1/-1) 488 ACTGG Cfr10I 16 GCCGGT [GA]^CCGG[CT] 233 ACCGGC Cfr13I 318 GGGCC G^G[ACGT]CC 333 GGACC HphI 99 GGTGA GGTGA(8/7) 116 TCACC MaeIII 494 GTAAC ^GT[ACGT]AC 689 GTAAC MwoI 134 GCTTTCTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 960 GCAACCATTGC NlaIV 317 GGGGCC GG[ACGT]^[ACGT]CC 912 GGATCC PleI 3 GACTC GAGTC(4/5) 835 GACTC Sau96I 318 GGGCC G^G[ACGT]CC 333 GGACC ScaI 221 AGTACT AGT^ACT 921 AGTACT SfaNI 151 GATGC GCATC(5/9) 284 GCATC StuI 200 AGGCCT AGG^CCT 555 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AfaI AluI AlwI BbvI BfaI BpmI BsaJI BsiYI BslI BssKI BstF5I BstNI BstYI Cac8I CfoI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MboI MboII MflI MnlI MslI MspI MvaI NdeII NlaIII NspI RsaI Sau3AI ScrFI SfcI TseI Tsp509I TspRI XhoII