(Circular) (Six-base) MAPSORT of: pFN21AE6382.seq Check: 7344 from: 1 to: 5686 pFN21AE6382 5686 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2710 2838 3029 1937 Size: 773 128 191 4594 Fragments arranged by size: 4594 773 191 128 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2324 2678 3749 5133 5401 1632 Size: 692 354 1071 1384 268 1917 Fragments arranged by size: 1917 1384 1071 692 354 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 2256 4585 5562 1203 Size: 1053 2329 977 1327 Fragments arranged by size: 2329 1327 1053 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 2402 1166 Size: 1236 4450 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5686 AlwNI CAG_nnn'CTG Cuts at: 2251 2302 3429 5001 2251 Size: 51 1127 1572 2936 Fragments arranged by size: 2936 1572 1127 51 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2595 2995 3221 3373 5482 1493 Size: 1102 400 226 152 2109 1697 Fragments arranged by size: 2109 1697 1102 400 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 3011 1870 Size: 73 1068 4545 Fragments arranged by size: 4545 1068 73 BamHI G'GATC_C Cuts at: 2917 3016 3383 2917 Size: 99 367 5220 Fragments arranged by size: 5220 367 99 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3007 4462 618 Size: 325 217 627 1220 1455 1842 Fragments arranged by size: 1842 1455 1220 627 325 217 BanII G_rGCy'C Cuts at: 1391 2064 3005 1391 Size: 673 941 4072 Fragments arranged by size: 4072 941 673 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3877 3911 1903 Size: 34 1940 34 3678 Fragments arranged by size: 3678 1940 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 2484 1548 Size: 936 4750 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2900 5681 2900 Size: 2781 2905 BlpI GC'TnA_GC Cuts at: 3103 3103 Size: 5686 Bme1580I G_kGCm'C Cuts at: 1790 3747 4903 1790 Size: 1957 1156 2573 Fragments arranged by size: 2573 1957 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3417 4375 442 Size: 416 53 83 399 24 2000 958 1753 Fragments arranged by size: 2000 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2078 2515 4346 1239 Size: 824 15 437 1831 2579 Fragments arranged by size: 2579 1831 824 437 15 Bpu10I CC'TnA_GC Cuts at: 2457 2457 Size: 5686 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2030 4349 882 Size: 617 531 2319 2219 Fragments arranged by size: 2319 2219 617 531 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 2905 5680 2905 Size: 2775 2911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3493 3875 4510 275 Size: 53 83 186 936 1960 382 635 1451 Fragments arranged by size: 1960 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4119 4517 4791 4938 5349 4119 Size: 398 274 147 411 4456 Fragments arranged by size: 4456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5461 Fragments arranged by size: 5461 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5686 BseYI C'CCAG_C Cuts at: 2578 4889 5385 2578 Size: 2311 496 2879 Fragments arranged by size: 2879 2311 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 1163 Size: 573 333 4780 Fragments arranged by size: 4780 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 3747 3832 4903 2064 Size: 1683 85 1071 2847 Fragments arranged by size: 2847 1683 1071 85 BsmI GAATG_Cn' Cuts at: 2763 3197 3290 2763 Size: 434 93 5159 Fragments arranged by size: 5159 434 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 3005 3747 3832 4903 1391 Size: 399 274 941 742 85 1071 2174 Fragments arranged by size: 2174 1071 941 742 399 274 85 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2382 3041 844 Size: 1538 659 3489 Fragments arranged by size: 3489 1538 659 BsrBI CCG'CTC Cuts at: 1394 1823 3573 1394 Size: 429 1750 3507 Fragments arranged by size: 3507 1750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2969 4181 4355 65 Size: 1183 1721 1212 174 1396 Fragments arranged by size: 1721 1396 1212 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2238 4330 4517 1700 Size: 110 211 217 2092 187 2869 Fragments arranged by size: 2869 2092 217 211 187 110 BsrGI T'GTAC_A Cuts at: 96 2634 96 Size: 2538 3148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 2971 794 Size: 2177 3509 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstYI r'GATC_y Cuts at: 1972 2452 2733 2900 2917 3016 3383 3768 3785 Size: 480 281 167 17 99 367 385 17 Cuts at: 3785 5237 5323 5335 5681 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 480 385 367 346 281 167 99 86 17 17 12 BtgI C'CryG_G Cuts at: 513 1065 2615 513 Size: 552 1550 3584 Fragments arranged by size: 3584 1550 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 2795 3202 3996 4016 2795 Size: 407 794 20 4465 Fragments arranged by size: 4465 794 407 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4693 809 Size: 507 3377 1802 Fragments arranged by size: 3377 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 3133 4022 Size: 54 1425 50 275 18 498 805 889 Cuts at: 4022 5651 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 805 498 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2891 3616 1353 Size: 1538 725 3423 Fragments arranged by size: 3423 1538 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2201 4273 4647 4793 387 Size: 1064 138 612 2072 374 146 1280 Fragments arranged by size: 2072 1280 1064 612 374 146 138 EcoICRI GAG'CTC Cuts at: 2062 2062 Size: 5686 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2078 2324 2515 2678 3749 4346 Size: 393 431 15 246 191 163 1071 597 Cuts at: 4346 5133 5401 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1071 787 597 431 393 268 246 191 163 15 EcoO109I rG'GnC_Cy Cuts at: 2323 2323 Size: 5686 EcoRI G'AATT_C Cuts at: 2595 2995 3373 2595 Size: 400 378 4908 Fragments arranged by size: 4908 400 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2329 2329 Size: 5686 FspI TGC'GCA Cuts at: 2147 4192 2147 Size: 2045 3641 HaeII r_GCGC'y Cuts at: 1678 4833 1678 Size: 3155 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2437 2469 4327 4359 4401 Size: 32 135 32 865 32 1858 32 42 Cuts at: 4401 4433 1373 Size: 32 2626 Fragments arranged by size: 2626 1858 865 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 2266 748 Size: 1518 4168 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 2377 3285 2377 Size: 908 4778 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2330 10 Size: 54 1425 343 498 3366 Fragments arranged by size: 3366 1425 498 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2811 2855 3644 4003 4162 518 Size: 648 1236 409 44 789 359 159 2042 Fragments arranged by size: 2042 1236 789 648 409 359 159 44 MspA1I CmG'CkG Cuts at: 1606 3102 3779 4927 5172 1606 Size: 1496 677 1148 245 2120 Fragments arranged by size: 2120 1496 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2615 513 Size: 552 1550 3584 Fragments arranged by size: 3584 1550 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 2260 2854 2965 3044 4589 1132 Size: 97 1031 594 111 79 1545 2229 Fragments arranged by size: 2229 1545 1031 594 111 97 79 PasI CC'CwG_GG Cuts at: 1348 2318 1348 Size: 970 4716 PciI A'CATG_T Cuts at: 2256 4585 2256 Size: 2329 3357 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5686 PfoI T'CCnGG_A Cuts at: 2097 2097 Size: 5686 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PstI C_TGCA'G Cuts at: 830 2568 3038 830 Size: 1738 470 3478 Fragments arranged by size: 3478 1738 470 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 SacI G_AGCT'C Cuts at: 2064 2064 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SbfI CC_TGCA'GG Cuts at: 2568 3038 2568 Size: 470 5216 ScaI AGT'ACT Cuts at: 2228 2928 3934 5646 2228 Size: 700 1006 1712 2268 Fragments arranged by size: 2268 1712 1006 700 SfcI C'TryA_G Cuts at: 826 1045 2564 2845 2922 3034 4169 4850 5041 Size: 219 1519 281 77 112 1135 681 191 Cuts at: 5041 826 Size: 1471 Fragments arranged by size: 1519 1471 1135 681 281 219 191 112 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 2854 3044 1132 Size: 1722 190 3774 Fragments arranged by size: 3774 1722 190 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 2207 2562 1517 Size: 690 355 4641 Fragments arranged by size: 4641 690 355 StyI C'CwwG_G Cuts at: 513 1065 2615 3125 513 Size: 552 1550 510 3074 Fragments arranged by size: 3074 1550 552 510 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 3730 3883 4068 4513 325 Size: 1255 2133 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2634 2829 Size: 274 80 33 51 1443 249 408 195 Cuts at: 2829 2926 3932 5644 96 Size: 97 1006 1712 138 Fragments arranged by size: 1712 1443 1006 408 274 249 195 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5646 5678 5646 Size: 32 5654 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 3815 3815 Size: 5686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI