################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbvCI BclI BfrI BglI BlnI BsaBI BsaI BseAI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tru9I XbaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 1931 GGCGCC G[GA]^CG[CT]C Alw44I 1735 GTGCAC G^TGCAC Aor51HI 1922 AGCGCT AGC^GCT ApaLI 1735 GTGCAC G^TGCAC AviII 1772 TGCGCA TGC^GCA BalI 710 TGGCCA TGG^CCA BbeI 1931 GGCGCC GGCGC^C BbsI 1234 GTCTTC GAAGAC(2/6) BcgI 1148 GCAGCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2402 GTATCC GTATCC(6/5) BlpI 1662 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1662 GCTCAGC GC^T[ACGT]AGC BpuAI 1234 GTCTTC GAAGAC(2/6) BsaHI 1931 GGCGCC G[GA]^CG[CT]C BsaWI 1142 ACCGGA [AT]^CCGG[AT] BspDI 2413 ATCGAT AT^CGAT BspLU11I 626 ACATGT A^CATGT BssSI 1762 CACGAG CACGAG(-5/-1) BstAPI 1335 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1662 GCTCAGC GC^T[ACGT]AGC ClaI 2413 ATCGAT AT^CGAT DsaI 2204 CCGTGG C^C[GA][CT]GG EagI 1561 CGGCCG C^GGCCG EclXI 1561 CGGCCG C^GGCCG Eco47III 1922 AGCGCT AGC^GCT Eco52I 1561 CGGCCG C^GGCCG FspI 1772 TGCGCA TGC^GCA Hin1I 1931 GGCGCC G[GA]^CG[CT]C KasI 1931 GGCGCC G^GCGCC MaeIII 498 GTCAC ^GT[ACGT]AC MluNI 710 TGGCCA TGG^CCA MscI 710 TGGCCA TGG^CCA NarI 1931 GGCGCC GG^CGCC PaeR7I 1438 CTCGAG C^TCGAG SbfI 150 CCTGCAGG CCTGCA^GG SfoI 1931 GGCGCC GGC^GCC SmaI 854 CCCGGG CCC^GGG SphI 254 GCATGC GCATG^C Sse8387I 150 CCTGCAGG CCTGCA^GG StuI 1397 AGGCCT AGG^CCT TfiI 426 GAATC G^A[AT]TC Tsp45I 498 GTCAC ^GT[GC]AC XhoI 1438 CTCGAG C^TCGAG XmaCI 854 CCCGGG C^CCGGG XmaI 854 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 937 GTCTAC GT^[AC][GT]AC 2359 GTCTAC AflIII 452 ACGTGT A^C[GA][CT]GT 626 ACATGT BbrPI 1168 CACGTG CAC^GTG 1981 CACGTG BfaI 196 CTAG C^TAG 247 CTAG BglII 1367 AGATCT A^GATCT 1583 AGATCT BsiEI 1561 CGGCCG CG[GA][CT]^CG 1881 CGACCG BsrBI 1572 CCGCTC CCGCTC(-3/-3) 1558 GAGCGG BsrDI 290 GCAATG GCAATG(2/0) 326 GCAATG BstXI 285 CCAAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 698 CCACTGAGATGG EcoRI 13 GAATTC G^AATTC 102 GAATTC EcoT14I 285 CCAAGG C^C[AT][AT]GG 2105 CCTTGG MaeI 196 CTAG C^TAG 247 CTAG NaeI 304 GCCGGC GCC^GGC 1928 GCCGGC NgoMIV 304 GCCGGC G^CCGGC 1928 GCCGGC PmaCI 1168 CACGTG CAC^GTG 1981 CACGTG PmlI 1168 CACGTG CAC^GTG 1981 CACGTG StyI 285 CCAAGG C^C[AT][AT]GG 2105 CCTTGG XcmI 698 CCACTGAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 884 CCAAGCGCAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AspI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp509I TspRI Tth111I TthHB8I Van91I XhoII XmnI