(Circular) (Six-base) MAPSORT of: pFN21AE4009.seq Check: 5966 from: 1 to: 7189 pFN21AE4009 7189 bp With 182 enzymes: SgfI * March 28, 2012 13:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2683 2922 3079 2683 Size: 239 157 6793 Fragments arranged by size: 6793 239 157 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4999 278 Size: 53 83 186 936 3463 2468 Fragments arranged by size: 3463 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2943 4365 4532 1937 Size: 1006 1422 167 4594 Fragments arranged by size: 4594 1422 1006 167 Acc65I G'GTAC_C Cuts at: 4510 4510 Size: 7189 AclI AA'CG_TT Cuts at: 1563 5316 5689 1563 Size: 3753 373 3063 Fragments arranged by size: 3753 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2100 2824 2995 5252 6636 6904 1632 Size: 468 724 171 2257 1384 268 1917 Fragments arranged by size: 2257 1917 1384 724 468 268 171 AfeI AGC'GCT Cuts at: 3929 3929 Size: 7189 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6992 AflIII A'CryG_T Cuts at: 1203 2457 2631 6088 7065 1203 Size: 1254 174 3457 977 1327 Fragments arranged by size: 3457 1327 1254 977 174 AgeI A'CCGG_T Cuts at: 6020 6020 Size: 7189 AhdI GACnn_n'nnGTC Cuts at: 5918 5918 Size: 7189 AleI CACnn'nnGTG Cuts at: 1166 2969 1166 Size: 1803 5386 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3435 1184 Size: 2251 4938 AlwNI CAG_nnn'CTG Cuts at: 2078 2561 2785 2874 3331 3346 4932 6504 2078 Size: 483 224 89 457 15 1586 1572 2763 Fragments arranged by size: 2763 1586 1572 483 457 224 89 15 ApaI G_GGCC'C Cuts at: 3028 3998 4065 3028 Size: 970 67 6152 Fragments arranged by size: 6152 970 67 ApaLI G'TGCA_C Cuts at: 3740 5246 6402 3740 Size: 1506 1156 4527 Fragments arranged by size: 4527 1506 1156 ApoI r'AATT_y Cuts at: 1493 2018 2107 2720 4498 4724 4876 6985 1493 Size: 525 89 613 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 613 525 226 152 89 AseI AT'TA_AT Cuts at: 160 1032 5743 160 Size: 872 4711 1606 Fragments arranged by size: 4711 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7189 AvaI C'yCGr_G Cuts at: 1870 1943 2240 2440 2831 2859 3263 3443 3752 Size: 73 297 200 391 28 404 180 309 Cuts at: 3752 4034 4076 4514 1870 Size: 282 42 438 4545 Fragments arranged by size: 4545 438 404 391 309 297 282 200 180 73 42 28 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4390 4423 4390 Size: 33 7156 BamHI G'GATC_C Cuts at: 4519 4886 4519 Size: 367 6822 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3598 3646 3653 3936 4147 Size: 325 217 627 1811 48 7 283 211 Cuts at: 4147 4428 4510 5965 618 Size: 281 82 1455 1842 Fragments arranged by size: 1842 1811 1455 627 325 283 281 217 211 82 48 7 BanII G_rGCy'C Cuts at: 1391 2358 2469 2702 2763 2838 3028 3270 3444 Size: 967 111 233 61 75 190 242 174 Cuts at: 3444 3759 3998 4065 4508 1391 Size: 315 239 67 443 4072 Fragments arranged by size: 4072 967 443 315 242 239 233 190 174 111 75 67 61 BbeI G_GCGC'C Cuts at: 3940 3940 Size: 7189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3232 928 Size: 971 1333 4885 Fragments arranged by size: 4885 1333 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3142 3176 5380 5414 1903 Size: 34 1205 34 2204 34 3678 Fragments arranged by size: 3678 2204 1205 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4418 5081 6291 4418 Size: 663 1210 5316 Fragments arranged by size: 5316 1210 663 BclI T'GATC_A Cuts at: 1548 1548 Size: 7189 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5800 136 Size: 107 122 71 631 605 4128 1525 Fragments arranged by size: 4128 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3372 3588 7184 3372 Size: 216 3596 3377 Fragments arranged by size: 3596 3377 216 BlpI GC'TnA_GC Cuts at: 3668 4606 3668 Size: 938 6251 Bme1580I G_kGCm'C Cuts at: 1790 3028 3424 3603 3651 3744 3998 4065 5250 Size: 1238 396 179 48 93 254 67 1185 Cuts at: 5250 6406 1790 Size: 1156 2573 Fragments arranged by size: 2573 1238 1185 1156 396 254 179 93 67 48 BmgBI CAC'GTC Cuts at: 1318 2625 2848 1318 Size: 1307 223 5659 Fragments arranged by size: 5659 1307 223 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4920 5878 442 Size: 416 53 83 399 24 3503 958 1753 Fragments arranged by size: 3503 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7189 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2146 2296 2146 Size: 150 7039 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2149 2364 2921 3493 3923 5849 1239 Size: 910 215 557 572 430 1926 2579 Fragments arranged by size: 2579 1926 910 572 557 430 215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2690 2747 4448 5314 6179 6477 2690 Size: 57 1701 866 865 298 3402 Fragments arranged by size: 3402 1701 866 865 298 57 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5852 882 Size: 617 4353 2219 Fragments arranged by size: 4353 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3175 3856 3988 4354 4980 493 Size: 711 1971 681 132 366 626 2702 Fragments arranged by size: 2702 1971 711 681 626 366 132 BsaBI GATnn'nnATC Cuts at: 7183 7183 Size: 7189 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4996 5378 6013 Size: 53 83 186 936 2404 1059 382 635 Cuts at: 6013 275 Size: 1451 Fragments arranged by size: 2404 1451 1059 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3147 5622 6020 6294 6441 6852 3147 Size: 2475 398 274 147 411 3484 Fragments arranged by size: 3484 2475 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2496 2526 2648 2678 3122 Size: 30 165 30 1093 30 122 30 444 Cuts at: 3122 3152 3200 3230 3491 3521 4376 4406 1178 Size: 30 48 30 261 30 855 30 3961 Fragments arranged by size: 3961 1093 855 444 261 165 122 48 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2239 2363 2366 3143 3208 3236 3410 3413 Size: 1053 124 3 777 65 28 174 3 Cuts at: 3413 3496 4064 4096 4223 4400 1186 Size: 83 568 32 127 177 3975 Fragments arranged by size: 3975 1053 777 568 177 174 127 124 83 65 32 28 3 3 BseYI C'CCAG_C Cuts at: 2637 2994 3027 3053 3286 3423 3630 4412 6392 Size: 357 33 26 233 137 207 782 1980 Cuts at: 6392 6888 2637 Size: 496 2938 Fragments arranged by size: 2938 1980 782 496 357 233 207 137 33 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2627 2840 2894 3878 4024 4214 1163 Size: 573 891 213 54 984 146 190 4138 Fragments arranged by size: 4138 984 891 573 213 190 146 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3569 3889 4639 5400 6428 1540 Size: 275 186 1568 320 750 761 1028 2301 Fragments arranged by size: 2301 1568 1028 761 750 320 275 186 BsiHKAI G_wGCw'C Cuts at: 2469 2763 2838 3270 3444 3744 3759 5250 5335 Size: 294 75 432 174 300 15 1491 85 Cuts at: 5335 6406 2469 Size: 1071 3252 Fragments arranged by size: 3252 1491 1071 432 300 294 174 85 75 15 BsmI GAATG_Cn' Cuts at: 4700 4793 4700 Size: 93 7096 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2358 2469 2702 2763 2838 3028 3270 Size: 399 568 111 233 61 75 190 242 Cuts at: 3270 3424 3444 3603 3651 3744 3759 3998 4065 Size: 154 20 159 48 93 15 239 67 Cuts at: 4065 4508 5250 5335 6406 1391 Size: 443 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 568 443 399 242 239 233 190 159 154 111 93 85 75 67 61 48 20 15 BspEI T'CCGG_A Cuts at: 6852 6852 Size: 7189 BspHI T'CATG_A Cuts at: 5078 6808 5078 Size: 1730 5459 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2457 2667 2683 2922 3079 3783 4170 4544 Size: 1613 210 16 239 157 704 387 374 Cuts at: 4544 844 Size: 3489 Fragments arranged by size: 3489 1613 704 387 374 239 210 157 16 BsrBI CCG'CTC Cuts at: 1394 1823 3565 3579 5076 1394 Size: 429 1742 14 1497 3507 Fragments arranged by size: 3507 1742 1497 429 14 BsrDI GCAATG_nn' Cuts at: 65 1248 2302 2338 5684 5858 65 Size: 1183 1054 36 3346 174 1396 Fragments arranged by size: 3346 1396 1183 1054 174 36 BsrFI r'CCGG_y Cuts at: 1700 1810 2309 3252 3933 5833 6020 1700 Size: 110 499 943 681 1900 187 2869 Fragments arranged by size: 2869 1900 943 681 499 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7189 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7189 BssSI C'ACGA_G Cuts at: 3767 5249 6261 3767 Size: 1482 1012 4695 Fragments arranged by size: 4695 1482 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3346 794 Size: 2552 4637 BstBI TT'CG_AA Cuts at: 5993 5993 Size: 7189 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7189 BstXI CCAn_nnnn'nTGG Cuts at: 2297 2710 2297 Size: 413 6776 BstYI r'GATC_y Cuts at: 1972 3372 3588 3624 4104 4519 4886 5271 5288 Size: 1400 216 36 480 415 367 385 17 Cuts at: 5288 6740 6826 6838 7184 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1400 480 415 385 367 346 216 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 4209 513 Size: 552 3144 3493 Fragments arranged by size: 3493 3144 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2205 3145 487 Size: 683 56 979 940 4531 Fragments arranged by size: 4531 979 940 683 56 BtsI GCAGTG_nn' Cuts at: 2148 2504 2875 3422 3475 4067 4705 5499 5519 Size: 356 371 547 53 592 638 794 20 Cuts at: 5519 2148 Size: 3818 Fragments arranged by size: 3818 794 638 592 547 371 356 53 20 ClaI AT'CG_AT Cuts at: 1554 4419 1554 Size: 2865 4324 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4461 4496 529 Size: 35 3897 35 3222 Fragments arranged by size: 3897 3222 35 35 DraI TTT'AAA Cuts at: 4493 4493 Size: 7189 DraIII CAC_nnn'GTG Cuts at: 2134 3178 3856 4354 2134 Size: 1044 678 498 4969 Fragments arranged by size: 4969 1044 678 498 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6196 809 Size: 507 4880 1802 Fragments arranged by size: 4880 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2715 2886 3058 Size: 54 1425 50 275 18 885 171 172 Cuts at: 3058 3100 3566 4636 5525 7154 8 Size: 42 466 1070 889 1629 43 Fragments arranged by size: 1629 1425 1070 889 885 466 275 172 171 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3566 4636 1537 Size: 275 1754 1070 4090 Fragments arranged by size: 4090 1754 1070 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2831 2843 3263 3275 3764 4262 5119 Size: 863 615 12 420 12 489 498 857 Cuts at: 5119 1353 Size: 3423 Fragments arranged by size: 3423 863 857 615 498 489 420 12 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3836 4229 4334 5776 6150 6296 Size: 1064 138 2247 393 105 1442 374 146 Cuts at: 6296 387 Size: 1280 Fragments arranged by size: 2247 1442 1280 1064 393 374 146 138 105 EcoICRI GAG'CTC Cuts at: 2467 2761 2836 3268 3442 3757 2467 Size: 294 75 432 174 315 5899 Fragments arranged by size: 5899 432 315 294 174 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2149 2364 2824 2921 2995 3493 Size: 393 468 49 215 460 97 74 498 Cuts at: 3493 3923 5252 5849 6636 6904 1239 Size: 430 1329 597 787 268 1524 Fragments arranged by size: 1524 1329 787 597 498 468 460 430 393 268 215 97 74 49 EcoO109I rG'GnC_Cy Cuts at: 2446 2797 3133 3190 3448 3995 4061 4062 2446 Size: 351 336 57 258 547 66 1 5573 Fragments arranged by size: 5573 547 351 336 258 66 57 1 EcoRI G'AATT_C Cuts at: 2018 2107 4498 4876 2018 Size: 89 2391 378 4331 Fragments arranged by size: 4331 2391 378 89 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4126 4126 Size: 7189 FspI TGC'GCA Cuts at: 3779 5695 3779 Size: 1916 5273 HaeII r_GCGC'y Cuts at: 1678 3931 3940 3961 6336 1678 Size: 2253 9 21 2375 2531 Fragments arranged by size: 2531 2375 2253 21 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 3122 3154 3200 Size: 32 135 32 1076 32 442 32 46 Cuts at: 3200 3232 3512 3544 5830 5862 5904 5936 1373 Size: 32 280 32 2286 32 42 32 2626 Fragments arranged by size: 2626 2286 1076 442 280 135 46 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4533 4779 669 Size: 988 281 2595 246 3079 Fragments arranged by size: 3079 2595 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7189 HpaI GTT'AAC Cuts at: 4779 4779 Size: 7189 KasI G'GCGC_C Cuts at: 3936 3936 Size: 7189 KpnI G_GTAC'C Cuts at: 4514 4514 Size: 7189 MfeI C'AATT_G Cuts at: 4788 4788 Size: 7189 MluI A'CGCG_T Cuts at: 7065 7065 Size: 7189 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2652 6303 6487 1305 Size: 1347 3651 184 2007 Fragments arranged by size: 3651 2007 1347 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2717 10 Size: 54 1425 343 885 4482 Fragments arranged by size: 4482 1425 885 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2708 2969 3530 5147 5506 5665 518 Size: 648 1542 261 561 1617 359 159 2042 Fragments arranged by size: 2042 1617 1542 648 561 359 261 159 MspA1I CmG'CkG Cuts at: 1606 2182 2641 2998 3031 3057 3303 3343 3798 Size: 576 459 357 33 26 246 40 455 Cuts at: 3798 3820 3862 4605 5282 6430 6675 1606 Size: 22 42 743 677 1148 245 2120 Fragments arranged by size: 2120 1148 743 677 576 459 455 357 246 245 42 40 33 26 22 NaeI GCC'GGC Cuts at: 2311 3935 2311 Size: 1624 5565 NarI GG'CG_CC Cuts at: 3937 3937 Size: 7189 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6637 NdeI CA'TA_TG Cuts at: 387 387 Size: 7189 NgoMIV G'CCGG_C Cuts at: 2309 3933 2309 Size: 1624 5565 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7189 NotI GC'GGCC_GC Cuts at: 4636 4636 Size: 7189 NspI r_CATG'y Cuts at: 1132 1229 2263 2611 2635 3391 4547 6092 1132 Size: 97 1034 348 24 756 1156 1545 2229 Fragments arranged by size: 2229 1545 1156 1034 756 348 97 24 PasI CC'CwG_GG Cuts at: 1348 3805 1348 Size: 2457 4732 PciI A'CATG_T Cuts at: 2631 6088 2631 Size: 3457 3732 PflMI CCAn_nnn'nTGG Cuts at: 1108 3109 3178 4354 1108 Size: 2001 69 1176 3943 Fragments arranged by size: 3943 2001 1176 69 PfoI T'CCnGG_A Cuts at: 2111 3108 3204 4044 2111 Size: 997 96 840 5256 Fragments arranged by size: 5256 997 840 96 PmeI GTTT'AAAC Cuts at: 4493 4493 Size: 7189 PmlI CAC'GTG Cuts at: 3175 3988 3175 Size: 813 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5258 5290 6771 6803 5258 Size: 32 1481 32 5644 Fragments arranged by size: 5644 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2446 2797 3190 2446 Size: 351 393 6445 Fragments arranged by size: 6445 393 351 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7189 PsiI TTA'TAA Cuts at: 4759 4759 Size: 7189 PspOMI G'GGCC_C Cuts at: 3024 3994 4061 3024 Size: 970 67 6152 Fragments arranged by size: 6152 970 67 PspXI vC'TCGA_Gb Cuts at: 3443 3443 Size: 7189 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2025 2057 2025 Size: 32 7157 PstI C_TGCA'G Cuts at: 830 2160 2870 2919 3351 3414 3797 4284 4541 Size: 1330 710 49 432 63 383 487 257 Cuts at: 4541 830 Size: 3478 Fragments arranged by size: 3478 1330 710 487 432 383 257 63 49 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7189 PvuII CAG'CTG Cuts at: 2182 2641 2998 3303 3820 3862 2182 Size: 459 357 305 517 42 5509 Fragments arranged by size: 5509 517 459 357 305 42 SacI G_AGCT'C Cuts at: 2469 2763 2838 3270 3444 3759 2469 Size: 294 75 432 174 315 5899 Fragments arranged by size: 5899 432 315 294 174 75 SalI G'TCGA_C Cuts at: 1936 4531 1936 Size: 2595 4594 SapI GCTCTTCn'nnn_ Cuts at: 2831 2843 3263 3275 3764 2831 Size: 12 420 12 489 6256 Fragments arranged by size: 6256 489 420 12 12 SbfI CC_TGCA'GG Cuts at: 2160 4541 2160 Size: 2381 4808 ScaI AGT'ACT Cuts at: 5437 7149 5437 Size: 1712 5477 SfcI C'TryA_G Cuts at: 826 1045 2156 2866 2915 3347 3410 3793 4280 Size: 219 1111 710 49 432 63 383 487 Cuts at: 4280 4537 5672 6353 6544 826 Size: 257 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 710 681 487 432 383 257 219 191 63 49 SfoI GGC'GCC Cuts at: 3938 3938 Size: 7189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7189 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7189 SmaI CCC'GGG Cuts at: 1872 2861 4516 1872 Size: 989 1655 4545 Fragments arranged by size: 4545 1655 989 SmlI C'TyrA_G Cuts at: 820 1017 1943 2669 2762 3443 4463 5293 6194 Size: 197 926 726 93 681 1020 830 901 Cuts at: 6194 6456 820 Size: 262 1553 Fragments arranged by size: 1553 1020 926 901 830 726 681 262 197 93 SnaBI TAC'GTA Cuts at: 493 4980 493 Size: 4487 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7189 SphI G_CATG'C Cuts at: 1132 2263 4547 1132 Size: 1131 2284 3774 Fragments arranged by size: 3774 2284 1131 SspI AAT'ATT Cuts at: 5 52 5113 5 Size: 47 5061 2081 Fragments arranged by size: 5061 2081 47 StuI AGG'CCT Cuts at: 1517 3404 1517 Size: 1887 5302 StyI C'CwwG_G Cuts at: 513 1065 2290 4110 4628 513 Size: 552 1225 1820 518 3074 Fragments arranged by size: 3074 1820 1225 552 518 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3278 5216 5233 5386 5571 6016 325 Size: 1255 1698 1938 17 153 185 445 1498 Fragments arranged by size: 1938 1698 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3297 3786 4284 Size: 274 80 33 51 1443 1320 489 498 Cuts at: 4284 5435 7147 96 Size: 1151 1712 138 Fragments arranged by size: 1712 1443 1320 1151 498 489 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2496 2528 4376 4408 7149 7181 2496 Size: 32 1848 32 2741 32 2504 Fragments arranged by size: 2741 2504 1848 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2141 2207 3890 1316 Size: 825 66 1683 4615 Fragments arranged by size: 4615 1683 825 66 XbaI T'CTAG_A Cuts at: 4525 4525 Size: 7189 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2710 2896 2710 Size: 186 7003 XhoI C'TCGA_G Cuts at: 1943 3443 1943 Size: 1500 5689 XmaI C'CCGG_G Cuts at: 1870 2859 4514 1870 Size: 989 1655 4545 Fragments arranged by size: 4545 1655 989 XmnI GAAnn'nnTTC Cuts at: 2040 2600 3005 5318 2040 Size: 560 405 2313 3911 Fragments arranged by size: 3911 2313 560 405 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4997 276 Size: 53 83 186 936 3463 2468 Fragments arranged by size: 3463 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI RsrII SacII SanDI SexAI SfiI SrfI SwaI