(Linear) (Six-base) MAPSORT of: KIEE4009.seq Check: 3840 from: 1 to: 2484 KIEE4009.orf With 182 enzymes: SgfI * March 22, 2012 17:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 678 917 1074 2484 Size: 678 239 157 1410 Fragments arranged by size: 1410 678 239 157 AccI GT'mk_AC Cuts at: 0 938 2360 2484 Size: 938 1422 124 Fragments arranged by size: 1422 938 124 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 95 819 990 2484 Size: 95 724 171 1494 Fragments arranged by size: 1494 724 171 95 AfeI AGC'GCT Cuts at: 0 1924 2484 Size: 1924 560 AflIII A'CryG_T Cuts at: 0 452 626 2484 Size: 452 174 1858 Fragments arranged by size: 1858 452 174 AleI CACnn'nnGTG Cuts at: 0 964 2484 Size: 964 1520 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1430 2484 Size: 1430 1054 AlwNI CAG_nnn'CTG Cuts at: 0 73 556 780 869 1326 1341 2484 Size: 73 483 224 89 457 15 1143 Fragments arranged by size: 1143 483 457 224 89 73 15 ApaI G_GGCC'C Cuts at: 0 1023 1993 2060 2484 Size: 1023 970 67 424 Fragments arranged by size: 1023 970 424 67 ApaLI G'TGCA_C Cuts at: 0 1735 2484 Size: 1735 749 ApoI r'AATT_y Cuts at: 0 13 102 715 2484 Size: 13 89 613 1769 Fragments arranged by size: 1769 613 89 13 AvaI C'yCGr_G Cuts at: 0 235 435 826 854 1258 1438 1747 2029 Size: 235 200 391 28 404 180 309 282 Cuts at: 2029 2071 2484 Size: 42 413 Fragments arranged by size: 413 404 391 309 282 235 200 180 42 28 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2385 2418 2484 Size: 2385 33 66 Fragments arranged by size: 2385 66 33 BanI G'GyrC_C Cuts at: 0 1593 1641 1648 1931 2142 2423 2484 Size: 1593 48 7 283 211 281 61 Fragments arranged by size: 1593 283 281 211 61 48 7 BanII G_rGCy'C Cuts at: 0 353 464 697 758 833 1023 1265 1439 Size: 353 111 233 61 75 190 242 174 Cuts at: 1439 1754 1993 2060 2484 Size: 315 239 67 424 Fragments arranged by size: 424 353 315 242 239 233 190 174 111 75 67 61 BbeI G_GCGC'C Cuts at: 0 1935 2484 Size: 1935 549 BbsI GAAGACnn'nnnn_ Cuts at: 0 1227 2484 Size: 1227 1257 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1137 1171 2484 Size: 1137 34 1313 Fragments arranged by size: 1313 1137 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2413 2484 Size: 2413 71 BglII A'GATC_T Cuts at: 0 1367 1583 2484 Size: 1367 216 901 Fragments arranged by size: 1367 901 216 BlpI GC'TnA_GC Cuts at: 0 1663 2484 Size: 1663 821 Bme1580I G_kGCm'C Cuts at: 0 1023 1419 1598 1646 1739 1993 2060 2484 Size: 1023 396 179 48 93 254 67 424 Fragments arranged by size: 1023 424 396 254 179 93 67 48 BmgBI CAC'GTC Cuts at: 0 620 843 2484 Size: 620 223 1641 Fragments arranged by size: 1641 620 223 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 141 291 2484 Size: 141 150 2193 Fragments arranged by size: 2193 150 141 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 359 916 1488 1918 2484 Size: 144 215 557 572 430 566 Fragments arranged by size: 572 566 557 430 215 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 685 742 2443 2484 Size: 685 57 1701 41 Fragments arranged by size: 1701 685 57 41 BsaAI yAC'GTr Cuts at: 0 1170 1851 1983 2349 2484 Size: 1170 681 132 366 135 Fragments arranged by size: 1170 681 366 135 132 BsaHI Gr'CG_yC Cuts at: 0 1932 2484 Size: 1932 552 BsaWI w'CCGG_w Cuts at: 0 1142 2484 Size: 1142 1342 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 491 521 643 673 1117 1147 1195 1225 Size: 491 30 122 30 444 30 48 30 Cuts at: 1225 1486 1516 2371 2401 2484 Size: 261 30 855 30 83 Fragments arranged by size: 855 491 444 261 122 83 48 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 234 358 361 1138 1203 1231 1405 1408 Size: 234 124 3 777 65 28 174 3 Cuts at: 1408 1491 2059 2091 2218 2395 2484 Size: 83 568 32 127 177 89 Fragments arranged by size: 777 568 234 177 174 127 124 89 83 65 32 28 3 3 BseYI C'CCAG_C Cuts at: 0 632 989 1022 1048 1281 1418 1625 2407 Size: 632 357 33 26 233 137 207 782 Cuts at: 2407 2484 Size: 77 Fragments arranged by size: 782 632 357 233 207 137 77 33 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 835 889 1873 2019 2209 2484 Size: 622 213 54 984 146 190 275 Fragments arranged by size: 984 622 275 213 190 146 54 BsiEI CG_ry'CG Cuts at: 0 1564 1884 2484 Size: 1564 320 600 Fragments arranged by size: 1564 600 320 BsiHKAI G_wGCw'C Cuts at: 0 464 758 833 1265 1439 1739 1754 2484 Size: 464 294 75 432 174 300 15 730 Fragments arranged by size: 730 464 432 300 294 174 75 15 Bsp1286I G_dGCh'C Cuts at: 0 353 464 697 758 833 1023 1265 1419 Size: 353 111 233 61 75 190 242 154 Cuts at: 1419 1439 1598 1646 1739 1754 1993 2060 2484 Size: 20 159 48 93 15 239 67 424 Fragments arranged by size: 424 353 242 239 233 190 159 154 111 93 75 67 61 48 20 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 452 662 678 917 1074 1778 2165 2484 Size: 452 210 16 239 157 704 387 319 Fragments arranged by size: 704 452 387 319 239 210 157 16 BsrBI CCG'CTC Cuts at: 0 1560 1574 2484 Size: 1560 14 910 Fragments arranged by size: 1560 910 14 BsrDI GCAATG_nn' Cuts at: 0 297 333 2484 Size: 297 36 2151 Fragments arranged by size: 2151 297 36 BsrFI r'CCGG_y Cuts at: 0 304 1247 1928 2484 Size: 304 943 681 556 Fragments arranged by size: 943 681 556 304 BssSI C'ACGA_G Cuts at: 0 1762 2484 Size: 1762 722 BstAPI GCAn_nnn'nTGC Cuts at: 0 1341 2484 Size: 1341 1143 BstXI CCAn_nnnn'nTGG Cuts at: 0 292 705 2484 Size: 292 413 1779 Fragments arranged by size: 1779 413 292 BstYI r'GATC_y Cuts at: 0 1367 1583 1619 2099 2484 Size: 1367 216 36 480 385 Fragments arranged by size: 1367 480 385 216 36 BtgI C'CryG_G Cuts at: 0 2204 2484 Size: 2204 280 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 200 1140 2484 Size: 200 940 1344 Fragments arranged by size: 1344 940 200 BtsI GCAGTG_nn' Cuts at: 0 143 499 870 1417 1470 2062 2484 Size: 143 356 371 547 53 592 422 Fragments arranged by size: 592 547 422 371 356 143 53 ClaI AT'CG_AT Cuts at: 0 2414 2484 Size: 2414 70 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2456 2484 Size: 2456 28 DraIII CAC_nnn'GTG Cuts at: 0 129 1173 1851 2349 2484 Size: 129 1044 678 498 135 Fragments arranged by size: 1044 678 498 135 129 EaeI y'GGCC_r Cuts at: 0 710 881 1053 1095 1561 2484 Size: 710 171 172 42 466 923 Fragments arranged by size: 923 710 466 172 171 42 EagI C'GGCC_G Cuts at: 0 1561 2484 Size: 1561 923 EarI CTCTTCn'nnn_ Cuts at: 0 211 826 838 1258 1270 1759 2257 2484 Size: 211 615 12 420 12 489 498 227 Fragments arranged by size: 615 498 489 420 227 211 12 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1831 2224 2329 2484 Size: 1831 393 105 155 Fragments arranged by size: 1831 393 155 105 EcoICRI GAG'CTC Cuts at: 0 462 756 831 1263 1437 1752 2484 Size: 462 294 75 432 174 315 732 Fragments arranged by size: 732 462 432 315 294 174 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 95 144 359 819 916 990 1488 1918 Size: 95 49 215 460 97 74 498 430 Cuts at: 1918 2484 Size: 566 Fragments arranged by size: 566 498 460 430 215 97 95 74 49 EcoO109I rG'GnC_Cy Cuts at: 0 441 792 1128 1185 1443 1990 2056 2057 Size: 441 351 336 57 258 547 66 1 Cuts at: 2057 2484 Size: 427 Fragments arranged by size: 547 441 427 351 336 258 66 57 1 EcoRI G'AATT_C Cuts at: 0 13 102 2484 Size: 13 89 2382 Fragments arranged by size: 2382 89 13 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2121 2484 Size: 2121 363 FspI TGC'GCA Cuts at: 0 1774 2484 Size: 1774 710 HaeII r_GCGC'y Cuts at: 0 1926 1935 1956 2484 Size: 1926 9 21 528 Fragments arranged by size: 1926 528 21 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 643 675 1117 1149 1195 1227 1507 1539 Size: 643 32 442 32 46 32 280 32 Cuts at: 1539 2484 Size: 945 Fragments arranged by size: 945 643 442 280 46 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1931 2484 Size: 1931 553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 647 2484 Size: 647 1837 MscI TGG'CCA Cuts at: 0 712 2484 Size: 712 1772 MslI CAynn'nnrTG Cuts at: 0 703 964 1525 2484 Size: 703 261 561 959 Fragments arranged by size: 959 703 561 261 MspA1I CmG'CkG Cuts at: 0 177 636 993 1026 1052 1298 1338 1793 Size: 177 459 357 33 26 246 40 455 Cuts at: 1793 1815 1857 2484 Size: 22 42 627 Fragments arranged by size: 627 459 455 357 246 177 42 40 33 26 22 NaeI GCC'GGC Cuts at: 0 306 1930 2484 Size: 306 1624 554 Fragments arranged by size: 1624 554 306 NarI GG'CG_CC Cuts at: 0 1932 2484 Size: 1932 552 NgoMIV G'CCGG_C Cuts at: 0 304 1928 2484 Size: 304 1624 556 Fragments arranged by size: 1624 556 304 NspI r_CATG'y Cuts at: 0 258 606 630 1386 2484 Size: 258 348 24 756 1098 Fragments arranged by size: 1098 756 348 258 24 PasI CC'CwG_GG Cuts at: 0 1800 2484 Size: 1800 684 PciI A'CATG_T Cuts at: 0 626 2484 Size: 626 1858 PflMI CCAn_nnn'nTGG Cuts at: 0 1104 1173 2349 2484 Size: 1104 69 1176 135 Fragments arranged by size: 1176 1104 135 69 PfoI T'CCnGG_A Cuts at: 0 106 1103 1199 2039 2484 Size: 106 997 96 840 445 Fragments arranged by size: 997 840 445 106 96 PmlI CAC'GTG Cuts at: 0 1170 1983 2484 Size: 1170 813 501 Fragments arranged by size: 1170 813 501 PpuMI rG'GwC_Cy Cuts at: 0 441 792 1185 2484 Size: 441 351 393 1299 Fragments arranged by size: 1299 441 393 351 PspOMI G'GGCC_C Cuts at: 0 1019 1989 2056 2484 Size: 1019 970 67 428 Fragments arranged by size: 1019 970 428 67 PspXI vC'TCGA_Gb Cuts at: 0 1438 2484 Size: 1438 1046 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 20 52 2484 Size: 20 32 2432 Fragments arranged by size: 2432 32 20 PstI C_TGCA'G Cuts at: 0 155 865 914 1346 1409 1792 2279 2484 Size: 155 710 49 432 63 383 487 205 Fragments arranged by size: 710 487 432 383 205 155 63 49 PvuII CAG'CTG Cuts at: 0 177 636 993 1298 1815 1857 2484 Size: 177 459 357 305 517 42 627 Fragments arranged by size: 627 517 459 357 305 177 42 SacI G_AGCT'C Cuts at: 0 464 758 833 1265 1439 1754 2484 Size: 464 294 75 432 174 315 730 Fragments arranged by size: 730 464 432 315 294 174 75 SapI GCTCTTCn'nnn_ Cuts at: 0 826 838 1258 1270 1759 2484 Size: 826 12 420 12 489 725 Fragments arranged by size: 826 725 489 420 12 12 SbfI CC_TGCA'GG Cuts at: 0 155 2484 Size: 155 2329 SfcI C'TryA_G Cuts at: 0 151 861 910 1342 1405 1788 2275 2484 Size: 151 710 49 432 63 383 487 209 Fragments arranged by size: 710 487 432 383 209 151 63 49 SfoI GGC'GCC Cuts at: 0 1933 2484 Size: 1933 551 SmaI CCC'GGG Cuts at: 0 856 2484 Size: 856 1628 SmlI C'TyrA_G Cuts at: 0 664 757 1438 2458 2484 Size: 664 93 681 1020 26 Fragments arranged by size: 1020 681 664 93 26 SphI G_CATG'C Cuts at: 0 258 2484 Size: 258 2226 StuI AGG'CCT Cuts at: 0 1399 2484 Size: 1399 1085 StyI C'CwwG_G Cuts at: 0 285 2105 2484 Size: 285 1820 379 Fragments arranged by size: 1820 379 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1273 2484 Size: 1273 1211 TatI w'GTAC_w Cuts at: 0 1292 1781 2279 2484 Size: 1292 489 498 205 Fragments arranged by size: 1292 498 489 205 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 491 523 2371 2403 2484 Size: 491 32 1848 32 81 Fragments arranged by size: 1848 491 81 32 32 Tth111I GACn'n_nGTC Cuts at: 0 136 202 1885 2484 Size: 136 66 1683 599 Fragments arranged by size: 1683 599 136 66 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 705 891 2484 Size: 705 186 1593 Fragments arranged by size: 1593 705 186 XhoI C'TCGA_G Cuts at: 0 1438 2484 Size: 1438 1046 XmaI C'CCGG_G Cuts at: 0 854 2484 Size: 854 1630 XmnI GAAnn'nnTTC Cuts at: 0 35 595 1000 2484 Size: 35 560 405 1484 Fragments arranged by size: 1484 560 405 35 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII BbeI BbsI BcgI BciVI BglII BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PsrI PstI PvuII SacI SapI SbfI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BamHI BbvCI BclI BfrBI BglI BmrI BmtI Bpu10I BsaI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I DraI DrdI EcoNI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI ZraI