Restriction map for
pFN21AE5723
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NciI NcoI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Alw44I 2405 GTGCAC G^TGCAC
AlwNI 1523 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2405 GTGCAC G^TGCAC
AseI 352 ATTAAT AT^TAAT
AsnI 352 ATTAAT AT^TAAT
Asp700I 1255 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1573 GGATCC G^GATCC
BanI 846 GGCACC G^G[CT][GA]CC
BanII 334 GAGCCC G[GA]GC[CT]^C
BbvCI 755 CCTCAGC CCTCAGC(-5/-2)
BsaBI 1222 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2191 GGTCTC GGTCTC(1/5)
BsaWI 1576 TCCGGT [AT]^CCGG[AT]
BsgI 1582 GTGCAG GTGCAG(16/14)
BsmFI 1796 GTCCC GGGAC(10/14)
Bsp1407I 1628 TGTACA T^GTACA
BspDI 1229 ATCGAT AT^CGAT
BspMI 1615 GCAGGT ACCTGC(4/8)
BsrGI 1628 TGTACA T^GTACA
BstAPI 2398 GCACTTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 270 CCAGGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1229 ATCGAT AT^CGAT
DraIII 2399 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1112 CCACGG C^C[GA][CT]GG
EarI 81 CTCTTC CTCTTC(1/4)
EcoRI 1428 GAATTC G^AATTC
EcoT14I 1634 CCAAGG C^C[AT][AT]GG
FauI 3 GCGGG CCCGC(4/6)
HapII 1577 CCGG C^CGG
HincII 1310 GTCAAC GT[CT]^[GA]AC
HindII 1310 GTCAAC GT[CT]^[GA]AC
HpaII 1577 CCGG C^CGG
Ksp632I 81 CTCTTC CTCTTC(1/4)
MaeII 1440 ACGT A^CGT
MamI 1222 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2014 CAATTG C^AATTG
MspI 1577 CCGG C^CGG
MunI 2014 CAATTG C^AATTG
NdeI 1016 CATATG CA^TATG
NheI 943 GCTAGC G^CTAGC
PshBI 352 ATTAAT AT^TAAT
ScaI 228 AGTACT AGT^ACT
SspBI 1628 TGTACA T^GTACA
StyI 1634 CCAAGG C^C[AT][AT]GG
XbaI 487 TCTAGA T^CTAGA
XmnI 1255 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 3 GCGG CCGC(-3/-1)
2323 GCGG
AflIII 117 ACATGT A^C[GA][CT]GT
2103 ACATGT
BbsI 76 GAAGAC GAAGAC(2/6)
2084 GTCTTC
BpuAI 76 GAAGAC GAAGAC(2/6)
2084 GTCTTC
BsiYI 717 CCCATTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
867 CCAGCAACAGG
BslI 717 CCCATTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
867 CCAGCAACAGG
BspLU11I 117 ACATGT A^CATGT
2103 ACATGT
BsrDI 1241 GCAATG GCAATG(2/0)
1496 GCAATG
BstF5I 274 GGATG GGATG(2/0)
906 GGATG
DraI 195 TTTAAA TTT^AAA
856 TTTAAA
FokI 274 GGATG GGATG(9/13)
906 GGATG
HindIII 23 AAGCTT A^AGCTT
1912 AAGCTT
MslI 1080 CACGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2356 CATGTTAGTG
MspA1I 709 CAGCTG C[AC]G^C[GT]G
2036 CAGCTG
PvuII 709 CAGCTG CAG^CTG
2036 CAGCTG
SfaNI 907 GATGC GCATC(5/9)
1504 GATGC
SfcI 98 CTGTAG C^T[GA][CT]AG
1250 CTATAG
SspI 1258 AATATT AAT^ATT
1744 AATATT
StuI 55 AGGCCT AGG^CCT
739 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BbvI BfaI BmyI
BpmI BsaJI BsiHKAI BsmAI BsmI
Bsp1286I BspHI BsrI BssKI BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII