(Circular) (Six-base) MAPSORT of: pFN21AE5723.seq Check: 4136 from: 1 to: 7147 pFN21AE5723 7147 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4957 278 Size: 53 83 186 936 3421 2468 Fragments arranged by size: 3421 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4490 1937 Size: 2553 4594 Acc65I G'GTAC_C Cuts at: 4468 4468 Size: 7147 AclI AA'CG_TT Cuts at: 1563 5274 5647 1563 Size: 3711 373 3063 Fragments arranged by size: 3711 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2073 3510 5210 6594 6862 1632 Size: 441 1437 1700 1384 268 1917 Fragments arranged by size: 1917 1700 1437 1384 441 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6950 AflIII A'CryG_T Cuts at: 1203 2122 4108 6046 7023 1203 Size: 919 1986 1938 977 1327 Fragments arranged by size: 1986 1938 1327 977 919 AgeI A'CCGG_T Cuts at: 5978 5978 Size: 7147 AhdI GACnn_n'nnGTC Cuts at: 5876 5876 Size: 7147 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4162 1184 Size: 2978 4169 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4170 4202 4170 Size: 32 7115 AlwNI CAG_nnn'CTG Cuts at: 3533 4890 6462 3533 Size: 1357 1572 4218 Fragments arranged by size: 4218 1572 1357 ApaLI G'TGCA_C Cuts at: 4410 5204 6360 4410 Size: 794 1156 5197 Fragments arranged by size: 5197 1156 794 ApoI r'AATT_y Cuts at: 1493 2560 2584 3433 4456 4682 4834 6943 1493 Size: 1067 24 849 1023 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 1023 849 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 2358 5701 160 Size: 872 1326 3343 1606 Fragments arranged by size: 3343 1606 1326 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7147 AvaI C'yCGr_G Cuts at: 1870 1943 4472 1870 Size: 73 2529 4545 Fragments arranged by size: 4545 2529 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3938 3971 3938 Size: 33 7114 BamHI G'GATC_C Cuts at: 3578 4477 4844 3578 Size: 899 367 5881 Fragments arranged by size: 5881 899 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2851 4468 5923 618 Size: 325 217 627 1064 1617 1455 1842 Fragments arranged by size: 1842 1617 1455 1064 627 325 217 BanII G_rGCy'C Cuts at: 1391 2343 4466 1391 Size: 952 2123 4072 Fragments arranged by size: 4072 2123 952 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 4082 928 Size: 971 189 1994 3993 Fragments arranged by size: 3993 1994 971 189 BbvCI CC'TCA_GC Cuts at: 2761 2761 Size: 7147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5338 5372 1903 Size: 34 50 34 3317 34 3678 Fragments arranged by size: 3678 3317 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5039 6249 5039 Size: 1210 5937 BclI T'GATC_A Cuts at: 1548 1548 Size: 7147 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5758 136 Size: 107 122 71 631 605 4086 1525 Fragments arranged by size: 4086 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7142 7142 Size: 7147 BlpI GC'TnA_GC Cuts at: 4564 4564 Size: 7147 Bme1580I G_kGCm'C Cuts at: 1790 4414 5208 6364 1790 Size: 2624 794 1156 2573 Fragments arranged by size: 2624 2573 1156 794 BmgBI CAC'GTC Cuts at: 1318 3446 1318 Size: 2128 5019 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4878 5836 Size: 416 53 83 399 24 2147 1314 958 Cuts at: 5836 442 Size: 1753 Fragments arranged by size: 2147 1753 1314 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2952 1056 Size: 1896 5251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2438 3378 3732 4258 5807 1239 Size: 1199 940 354 526 1549 2579 Fragments arranged by size: 2579 1549 1199 940 526 354 Bpu10I CC'TnA_GC Cuts at: 2761 2761 Size: 7147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2246 2672 4105 5272 6137 6435 2246 Size: 426 1433 1167 865 298 2958 Fragments arranged by size: 2958 1433 1167 865 426 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4202 5810 882 Size: 617 2703 1608 2219 Fragments arranged by size: 2703 2219 1608 617 BsaAI yAC'GTr Cuts at: 493 1204 4938 493 Size: 711 3734 2702 Fragments arranged by size: 3734 2702 711 BsaBI GATnn'nnATC Cuts at: 3231 7141 3231 Size: 3910 3237 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4954 5336 5971 275 Size: 53 83 186 936 3421 382 635 1451 Fragments arranged by size: 3421 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3581 5580 5978 6252 6399 6810 3581 Size: 1999 398 274 147 411 3918 Fragments arranged by size: 3918 1999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3351 3381 4170 4200 1178 Size: 30 165 30 1948 30 789 30 4125 Fragments arranged by size: 4125 1948 789 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7147 BseYI C'CCAG_C Cuts at: 6350 6846 6350 Size: 496 6651 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3608 1163 Size: 573 1872 4702 Fragments arranged by size: 4702 1872 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4597 5358 6386 1540 Size: 275 186 2596 761 1028 2301 Fragments arranged by size: 2596 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2369 4405 4414 5208 5293 6364 2369 Size: 2036 9 794 85 1071 3152 Fragments arranged by size: 3152 2036 1071 794 85 9 BsmI GAATG_Cn' Cuts at: 2952 3292 4171 4658 4751 2952 Size: 340 879 487 93 5348 Fragments arranged by size: 5348 879 487 340 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2343 2369 4405 4414 4466 5208 5293 Size: 399 553 26 2036 9 52 742 85 Cuts at: 5293 6364 1391 Size: 1071 2174 Fragments arranged by size: 2174 2036 1071 742 553 399 85 52 26 9 BspEI T'CCGG_A Cuts at: 6810 6810 Size: 7147 BspHI T'CATG_A Cuts at: 2074 2499 3229 3325 3429 5036 6766 2074 Size: 425 730 96 104 1607 1730 2455 Fragments arranged by size: 2455 1730 1607 730 425 104 96 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3611 4502 844 Size: 2767 891 3489 Fragments arranged by size: 3489 2767 891 BsrBI CCG'CTC Cuts at: 1394 1823 5034 1394 Size: 429 3211 3507 Fragments arranged by size: 3507 3211 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3253 3508 5642 5816 65 Size: 1183 2005 255 2134 174 1396 Fragments arranged by size: 2134 2005 1396 1183 255 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5791 5978 1700 Size: 110 3981 187 2869 Fragments arranged by size: 3981 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3633 96 Size: 3537 3610 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7147 BssSI C'ACGA_G Cuts at: 5207 6219 5207 Size: 1012 6135 BstAPI GCAn_nnn'nTGC Cuts at: 794 4409 794 Size: 3615 3532 BstBI TT'CG_AA Cuts at: 5951 5951 Size: 7147 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7147 BstXI CCAn_nnnn'nTGG Cuts at: 2282 2282 Size: 7147 BstYI r'GATC_y Cuts at: 1972 2017 3060 3127 3566 3578 4477 4844 5229 Size: 45 1043 67 439 12 899 367 385 Cuts at: 5229 5246 6698 6784 6796 7142 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1043 899 439 385 367 346 86 67 45 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 3117 513 Size: 552 2052 4543 Fragments arranged by size: 4543 2052 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6408 Fragments arranged by size: 6408 683 56 BtsI GCAGTG_nn' Cuts at: 2922 4245 4663 5457 5477 2922 Size: 1323 418 794 20 4592 Fragments arranged by size: 4592 1323 794 418 20 ClaI AT'CG_AT Cuts at: 1554 3235 1554 Size: 1681 5466 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7112 DraI TTT'AAA Cuts at: 2202 2863 4451 2202 Size: 661 1588 4898 Fragments arranged by size: 4898 1588 661 DraIII CAC_nnn'GTG Cuts at: 4409 4409 Size: 7147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6154 809 Size: 507 4838 1802 Fragments arranged by size: 4838 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4594 5483 7112 Size: 54 1425 50 275 18 2764 889 1629 Cuts at: 7112 8 Size: 43 Fragments arranged by size: 2764 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4594 1537 Size: 275 2782 4090 Fragments arranged by size: 4090 2782 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2092 5077 1353 Size: 739 2985 3423 Fragments arranged by size: 3423 2985 739 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5734 6108 6254 387 Size: 1064 138 4145 374 146 1280 Fragments arranged by size: 4145 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2438 3378 3510 3732 4258 5210 Size: 393 441 365 940 132 222 526 952 Cuts at: 5210 5807 6594 6862 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 952 940 787 597 526 441 393 365 268 222 132 EcoRI G'AATT_C Cuts at: 3433 4456 4834 3433 Size: 1023 378 5746 Fragments arranged by size: 5746 1023 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2287 2828 4439 2287 Size: 541 1611 4995 Fragments arranged by size: 4995 1611 541 FspI TGC'GCA Cuts at: 5653 5653 Size: 7147 HaeII r_GCGC'y Cuts at: 1678 6294 1678 Size: 4616 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 2626 2658 3351 Size: 32 135 32 497 32 525 32 693 Cuts at: 3351 3383 3811 3843 4325 4357 5788 5820 5862 Size: 32 428 32 482 32 1431 32 42 Cuts at: 5862 5894 1373 Size: 32 2626 Fragments arranged by size: 2626 1431 693 525 497 482 428 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3317 4491 4737 669 Size: 988 281 1379 1174 246 3079 Fragments arranged by size: 3079 1379 1174 988 281 246 HindIII A'AGCT_T Cuts at: 748 2028 3917 748 Size: 1280 1889 3978 Fragments arranged by size: 3978 1889 1280 HpaI GTT'AAC Cuts at: 4737 4737 Size: 7147 KpnI G_GTAC'C Cuts at: 4472 4472 Size: 7147 MfeI C'AATT_G Cuts at: 4019 4746 4019 Size: 727 6420 MluI A'CGCG_T Cuts at: 7023 7023 Size: 7147 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2045 6261 6445 1305 Size: 740 4216 184 2007 Fragments arranged by size: 4216 2007 740 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5325 Fragments arranged by size: 5325 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3089 4365 5105 5464 5623 518 Size: 648 843 1080 1276 740 359 159 2042 Fragments arranged by size: 2042 1276 1080 843 740 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2716 4043 4563 5240 6388 6633 1606 Size: 1110 1327 520 677 1148 245 2120 Fragments arranged by size: 2120 1327 1148 1110 677 520 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6595 NdeI CA'TA_TG Cuts at: 387 3022 387 Size: 2635 4512 NheI G'CTAG_C Cuts at: 1052 2948 1052 Size: 1896 5251 NotI GC'GGCC_GC Cuts at: 4594 4594 Size: 7147 NspI r_CATG'y Cuts at: 1132 1229 2126 2465 4112 4505 6050 1132 Size: 97 897 339 1647 393 1545 2229 Fragments arranged by size: 2229 1647 1545 897 393 339 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7147 PciI A'CATG_T Cuts at: 2122 4108 6046 2122 Size: 1986 1938 3223 Fragments arranged by size: 3223 1986 1938 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7147 PfoI T'CCnGG_A Cuts at: 4274 4274 Size: 7147 PmeI GTTT'AAAC Cuts at: 4451 4451 Size: 7147 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4170 4202 5216 5248 6729 6761 4170 Size: 32 1014 32 1481 32 4556 Fragments arranged by size: 4556 1481 1014 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7147 PsiI TTA'TAA Cuts at: 2354 4717 2354 Size: 2363 4784 PstI C_TGCA'G Cuts at: 830 4499 830 Size: 3669 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7147 PvuII CAG'CTG Cuts at: 2716 4043 2716 Size: 1327 5820 SalI G'TCGA_C Cuts at: 1936 4489 1936 Size: 2553 4594 SbfI CC_TGCA'GG Cuts at: 4499 4499 Size: 7147 ScaI AGT'ACT Cuts at: 2235 5395 7107 2235 Size: 3160 1712 2275 Fragments arranged by size: 3160 2275 1712 SfcI C'TryA_G Cuts at: 826 1045 2103 3255 4495 5630 6311 6502 826 Size: 219 1058 1152 1240 1135 681 191 1471 Fragments arranged by size: 1471 1240 1152 1135 1058 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7147 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7147 SmaI CCC'GGG Cuts at: 1872 4474 1872 Size: 2602 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 2687 4084 5251 6152 6414 Size: 197 926 318 426 1397 1167 901 262 Cuts at: 6414 820 Size: 1553 Fragments arranged by size: 1553 1397 1167 926 901 426 318 262 197 SnaBI TAC'GTA Cuts at: 493 4938 493 Size: 4445 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7147 SphI G_CATG'C Cuts at: 1132 4505 1132 Size: 3373 3774 SspI AAT'ATT Cuts at: 5 52 3265 3751 5071 5 Size: 47 3213 486 1320 2081 Fragments arranged by size: 3213 2081 1320 486 47 StuI AGG'CCT Cuts at: 1517 2062 2746 1517 Size: 545 684 5918 Fragments arranged by size: 5918 684 545 StyI C'CwwG_G Cuts at: 513 1065 3639 4586 513 Size: 552 2574 947 3074 Fragments arranged by size: 3074 2574 947 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3345 5174 5191 5344 5529 5974 325 Size: 1255 1765 1829 17 153 185 445 1498 Fragments arranged by size: 1829 1765 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2233 2678 3633 Size: 274 80 33 51 1443 256 445 955 Cuts at: 3633 3935 5393 7105 96 Size: 302 1458 1712 138 Fragments arranged by size: 1712 1458 1443 955 445 302 274 256 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3570 3602 7107 7139 3570 Size: 32 3505 32 3578 Fragments arranged by size: 3578 3505 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7147 XbaI T'CTAG_A Cuts at: 2492 4483 2492 Size: 1991 5156 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7147 XmaI C'CCGG_G Cuts at: 1870 4472 1870 Size: 2602 4545 XmnI GAAnn'nnTTC Cuts at: 3264 5276 3264 Size: 2012 5135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4955 276 Size: 53 83 186 936 3421 2468 Fragments arranged by size: 3421 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI