(Linear) (Six-base) MAPSORT of: KIEE5723.seq Check: 6693 from: 1 to: 2442 KIEE5723.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 1505 2442 Size: 68 1437 937 Fragments arranged by size: 1437 937 68 AflIII A'CryG_T Cuts at: 0 117 2103 2442 Size: 117 1986 339 Fragments arranged by size: 1986 339 117 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2157 2442 Size: 2157 285 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2165 2197 2442 Size: 2165 32 245 Fragments arranged by size: 2165 245 32 AlwNI CAG_nnn'CTG Cuts at: 0 1528 2442 Size: 1528 914 ApaLI G'TGCA_C Cuts at: 0 2405 2442 Size: 2405 37 ApoI r'AATT_y Cuts at: 0 555 579 1428 2442 Size: 555 24 849 1014 Fragments arranged by size: 1014 849 555 24 AseI AT'TA_AT Cuts at: 0 353 2442 Size: 353 2089 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1933 1966 2442 Size: 1933 33 476 Fragments arranged by size: 1933 476 33 BamHI G'GATC_C Cuts at: 0 1573 2442 Size: 1573 869 BanI G'GyrC_C Cuts at: 0 846 2442 Size: 846 1596 BanII G_rGCy'C Cuts at: 0 338 2442 Size: 338 2104 BbsI GAAGACnn'nnnn_ Cuts at: 0 83 2077 2442 Size: 83 1994 365 Fragments arranged by size: 1994 365 83 BbvCI CC'TCA_GC Cuts at: 0 756 2442 Size: 756 1686 Bme1580I G_kGCm'C Cuts at: 0 2409 2442 Size: 2409 33 BmgBI CAC'GTC Cuts at: 0 1441 2442 Size: 1441 1001 BmrI ACTGGGnnnn_n' Cuts at: 0 1559 2442 Size: 1559 883 BmtI G_CTAG'C Cuts at: 0 947 2442 Size: 947 1495 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 1373 1727 2253 2442 Size: 433 940 354 526 189 Fragments arranged by size: 940 526 433 354 189 Bpu10I CC'TnA_GC Cuts at: 0 756 2442 Size: 756 1686 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 667 2100 2442 Size: 241 426 1433 342 Fragments arranged by size: 1433 426 342 241 BsaI GGTCTCn'nnnn_ Cuts at: 0 2197 2442 Size: 2197 245 BsaBI GATnn'nnATC Cuts at: 0 1226 2442 Size: 1226 1216 BsaWI w'CCGG_w Cuts at: 0 1576 2442 Size: 1576 866 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1346 1376 2165 2195 2442 Size: 1346 30 789 30 247 Fragments arranged by size: 1346 789 247 30 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1603 2442 Size: 1603 839 BsiHKAI G_wGCw'C Cuts at: 0 364 2400 2409 2442 Size: 364 2036 9 33 Fragments arranged by size: 2036 364 33 9 BsmI GAATG_Cn' Cuts at: 0 947 1287 2166 2442 Size: 947 340 879 276 Fragments arranged by size: 947 879 340 276 Bsp1286I G_dGCh'C Cuts at: 0 338 364 2400 2409 2442 Size: 338 26 2036 9 33 Fragments arranged by size: 2036 338 33 26 9 BspHI T'CATG_A Cuts at: 0 69 494 1224 1320 1424 2442 Size: 69 425 730 96 104 1018 Fragments arranged by size: 1018 730 425 104 96 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1606 2442 Size: 1606 836 BsrDI GCAATG_nn' Cuts at: 0 1248 1503 2442 Size: 1248 255 939 Fragments arranged by size: 1248 939 255 BsrGI T'GTAC_A Cuts at: 0 1628 2442 Size: 1628 814 BstAPI GCAn_nnn'nTGC Cuts at: 0 2404 2442 Size: 2404 38 BstXI CCAn_nnnn'nTGG Cuts at: 0 277 2442 Size: 277 2165 BstYI r'GATC_y Cuts at: 0 12 1055 1122 1561 1573 2442 Size: 12 1043 67 439 12 869 Fragments arranged by size: 1043 869 439 67 12 12 BtgI C'CryG_G Cuts at: 0 1112 2442 Size: 1112 1330 BtsI GCAGTG_nn' Cuts at: 0 917 2240 2442 Size: 917 1323 202 Fragments arranged by size: 1323 917 202 ClaI AT'CG_AT Cuts at: 0 1230 2442 Size: 1230 1212 DraI TTT'AAA Cuts at: 0 197 858 2442 Size: 197 661 1584 Fragments arranged by size: 1584 661 197 DraIII CAC_nnn'GTG Cuts at: 0 2404 2442 Size: 2404 38 EarI CTCTTCn'nnn_ Cuts at: 0 87 2442 Size: 87 2355 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 433 1373 1505 1727 2253 2442 Size: 68 365 940 132 222 526 189 Fragments arranged by size: 940 526 365 222 189 132 68 EcoRI G'AATT_C Cuts at: 0 1428 2442 Size: 1428 1014 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 282 823 2434 2442 Size: 282 541 1611 8 Fragments arranged by size: 1611 541 282 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 64 96 621 653 1346 1378 1806 1838 Size: 64 32 525 32 693 32 428 32 Cuts at: 1838 2320 2352 2442 Size: 482 32 90 Fragments arranged by size: 693 525 482 428 90 64 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1312 2442 Size: 1312 1130 HindIII A'AGCT_T Cuts at: 0 23 1912 2442 Size: 23 1889 530 Fragments arranged by size: 1889 530 23 MfeI C'AATT_G Cuts at: 0 2014 2442 Size: 2014 428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 40 2442 Size: 40 2402 MslI CAynn'nnrTG Cuts at: 0 1084 2360 2442 Size: 1084 1276 82 Fragments arranged by size: 1276 1084 82 MspA1I CmG'CkG Cuts at: 0 711 2038 2442 Size: 711 1327 404 Fragments arranged by size: 1327 711 404 NdeI CA'TA_TG Cuts at: 0 1017 2442 Size: 1017 1425 NheI G'CTAG_C Cuts at: 0 943 2442 Size: 943 1499 NspI r_CATG'y Cuts at: 0 121 460 2107 2442 Size: 121 339 1647 335 Fragments arranged by size: 1647 339 335 121 PciI A'CATG_T Cuts at: 0 117 2103 2442 Size: 117 1986 339 Fragments arranged by size: 1986 339 117 PfoI T'CCnGG_A Cuts at: 0 2269 2442 Size: 2269 173 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2165 2197 2442 Size: 2165 32 245 Fragments arranged by size: 2165 245 32 PsiI TTA'TAA Cuts at: 0 349 2442 Size: 349 2093 PvuII CAG'CTG Cuts at: 0 711 2038 2442 Size: 711 1327 404 Fragments arranged by size: 1327 711 404 ScaI AGT'ACT Cuts at: 0 230 2442 Size: 230 2212 SfcI C'TryA_G Cuts at: 0 98 1250 2442 Size: 98 1152 1192 Fragments arranged by size: 1192 1152 98 SmlI C'TyrA_G Cuts at: 0 256 682 2079 2442 Size: 256 426 1397 363 Fragments arranged by size: 1397 426 363 256 SspI AAT'ATT Cuts at: 0 1260 1746 2442 Size: 1260 486 696 Fragments arranged by size: 1260 696 486 StuI AGG'CCT Cuts at: 0 57 741 2442 Size: 57 684 1701 Fragments arranged by size: 1701 684 57 StyI C'CwwG_G Cuts at: 0 1634 2442 Size: 1634 808 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1340 2442 Size: 1340 1102 TatI w'GTAC_w Cuts at: 0 228 673 1628 1930 2442 Size: 228 445 955 302 512 Fragments arranged by size: 955 512 445 302 228 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1565 1597 2442 Size: 1565 32 845 Fragments arranged by size: 1565 845 32 XbaI T'CTAG_A Cuts at: 0 487 2442 Size: 487 1955 XmnI GAAnn'nnTTC Cuts at: 0 1259 2442 Size: 1259 1183 Enzymes that do cut: AcuI AflIII AlfI AloI AlwNI ApaLI ApoI AseI BaeI BamHI BanI BanII BbsI BbvCI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstXI BstYI BtgI BtsI ClaI DraI DraIII EarI Eco57MI EcoRI FalI Hin4I HincII HindIII MfeI MmeI MslI MspA1I NdeI NheI NspI PciI PfoI PpiI PsiI PvuII ScaI SfcI SmlI SspI StuI StyI TaqII TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI ApaI AscI AsiSI AvaI AvrII BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BplI BsaAI BsaHI BseRI BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XcmI XhoI XmaI ZraI