################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AvaII AviII AvrII BamHI BbrPI BclI BfrI BglI BlnI BsaBI BseAI BseRI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BstBI Bsu36I ClaI CpoI DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoRI EcoT14I EcoT22I FbaI FseI FspI HincII HindII HpaI Ksp632I MamI MfeI MluI MroI MslI MunI NaeI NcoI NgoMIV NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI StyI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 411 GTATAC GT^[AC][GT]AC AlwNI 1480 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 646 ATTAAT AT^TAAT AsnI 646 ATTAAT AT^TAAT BbeI 1644 GGCGCC GGCGC^C BbvCI 1494 CCTCAGC CCTCAGC(-5/-2) BcgI 934 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1196 GGATAC GTATCC(6/5) BglII 756 AGATCT A^GATCT BlpI 1530 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1530 GCTGAGC GC^T[ACGT]AGC BsaI 1016 GAGACC GGTCTC(1/5) BsaWI 1411 ACCGGA [AT]^CCGG[AT] BsmBI 1677 CGTCTC CGTCTC(1/5) BspMI 1653 GCAGGT ACCTGC(4/8) BssHII 1363 GCGCGC G^CGCGC Bst1107I 411 GTATAC GTA^TAC BstEII 1407 GGTGACC G^GT[ACGT]ACC BstPI 1407 GGTGACC G^GT[ACGT]ACC BstXI 170 CCAACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 411 GTATAC GTA^TAC CelII 1530 GCTGAGC GC^T[ACGT]AGC EcoO65I 1407 GGTGACC G^GT[ACGT]ACC HindIII 1039 AAGCTT A^AGCTT KasI 1644 GGCGCC G^GCGCC KspI 1268 CCGCGG CCGC^GG NarI 1644 GGCGCC GG^CGCC NdeI 377 CATATG CA^TATG NheI 1378 GCTAGC G^CTAGC NotI 1274 GCGGCCGC GC^GGCCGC PflMI 1328 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 646 ATTAAT AT^TAAT PvuII 1564 CAGCTG CAG^CTG SacI 1768 GAGCTC GAGCT^C SacII 1268 CCGCGG CCGC^GG SfoI 1644 GGCGCC GGC^GCC SphI 1149 GCATGC GCATG^C TaqI 1837 TCGA T^CGA TthHB8I 1837 TCGA T^CGA Van91I 1328 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 232 GGTACC G^GTACC 781 GGTACC Asp718I 232 GGTACC G^GTACC 781 GGTACC AspI 220 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC 799 GACCTGGTC BalI 362 TGGCCA TGG^CCA 1205 TGGCCA BbsI 1117 GAAGAC GAAGAC(2/6) 1344 GAAGAC BpmI 1306 CTGGAG CTGGAG(16/14) 1354 CTGGAG BpuAI 1117 GAAGAC GAAGAC(2/6) 1344 GAAGAC BsaAI 1315 TACGTG [CT]AC^GT[GA] 1546 TACGTG BsmFI 966 GGGAC GGGAC(10/14) 990 GTCCC BsmI 90 GAATGC GAATGC(1/-1) 697 GCATTC BsrFI 1108 GCCGGT [GA]^CCGG[CT] 1624 ACCGGC BssSI 1258 CTCGTG CACGAG(-5/-1) 1473 CTCGTG Cfr10I 1108 GCCGGT [GA]^CCGG[CT] 1624 ACCGGC DsaI 1268 CCGCGG C^C[GA][CT]GG 1601 CCGTGG EagI 1275 CGGCCG C^GGCCG 1395 CGGCCG EclXI 1275 CGGCCG C^GGCCG 1395 CGGCCG Eco52I 1275 CGGCCG C^GGCCG 1395 CGGCCG EcoRV 40 GATATC GAT^ATC 343 GATATC HaeII 1644 GGCGCC [GA]GCGC^[CT] 1683 GGCGCT KpnI 232 GGTACC GGTAC^C 781 GGTACC MluNI 362 TGGCCA TGG^CCA 1205 TGGCCA MscI 362 TGGCCA TGG^CCA 1205 TGGCCA NspI 515 GCATGT [GA]CATG^[CT] 1149 GCATGC PflFI 220 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC 799 GACCTGGTC SexAI 800 ACCTGGT A^CC[AT]GGT 1703 ACCTGGT SmaI 1505 CCCGGG CCC^GGG 1865 CCCGGG SspI 529 AATATT AAT^ATT 769 AATATT StuI 357 AGGCCT AGG^CCT 975 AGGCCT Tth111I 220 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC 799 GACCTGGTC XmaCI 1505 CCCGGG C^CCGGG 1865 CCCGGG XmaI 1505 CCCGGG C^CCGGG 1865 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaI BanI BanII BbvI BcnI BfaI BmyI BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII