(Circular) (Six-base) MAPSORT of: pFN21AE4174.seq Check: 8409 from: 1 to: 6607 pFN21AE4174 6607 bp With 182 enzymes: SgfI * March 28, 2012 13:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3649 3649 Size: 6607 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4417 278 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2417 3950 1937 Size: 480 1533 4594 Fragments arranged by size: 4594 1533 480 Acc65I G'GTAC_C Cuts at: 2237 2786 3928 2237 Size: 549 1142 4916 Fragments arranged by size: 4916 1142 549 AclI AA'CG_TT Cuts at: 1563 4734 5107 1563 Size: 3171 373 3063 Fragments arranged by size: 3171 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 3223 3748 4670 6054 6322 1632 Size: 806 785 525 922 1384 268 1917 Fragments arranged by size: 1917 1384 922 806 785 525 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6410 AflIII A'CryG_T Cuts at: 1203 5506 6483 1203 Size: 4303 977 1327 Fragments arranged by size: 4303 1327 977 AgeI A'CCGG_T Cuts at: 5438 5438 Size: 6607 AhdI GACnn_n'nnGTC Cuts at: 5336 5336 Size: 6607 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6607 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6607 AlwNI CAG_nnn'CTG Cuts at: 3490 4350 5922 3490 Size: 860 1572 4175 Fragments arranged by size: 4175 1572 860 ApaLI G'TGCA_C Cuts at: 4664 5820 4664 Size: 1156 5451 ApoI r'AATT_y Cuts at: 1493 2582 2606 2729 3916 4142 4294 6403 1493 Size: 1089 24 123 1187 226 152 2109 1697 Fragments arranged by size: 2109 1697 1187 1089 226 152 123 24 AseI AT'TA_AT Cuts at: 160 1032 2652 5161 160 Size: 872 1620 2509 1606 Fragments arranged by size: 2509 1620 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 AvaI C'yCGr_G Cuts at: 1870 1943 3510 3770 3806 3861 3870 3932 1870 Size: 73 1567 260 36 55 9 62 4545 Fragments arranged by size: 4545 1567 260 73 62 55 36 9 BamHI G'GATC_C Cuts at: 3937 4304 3937 Size: 367 6240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2237 2786 3649 3928 Size: 325 217 627 221 229 549 863 279 Cuts at: 3928 5383 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 863 627 549 325 279 229 221 217 BanII G_rGCy'C Cuts at: 1391 2112 3264 3777 3926 1391 Size: 721 1152 513 149 4072 Fragments arranged by size: 4072 1152 721 513 149 BbeI G_GCGC'C Cuts at: 3653 3653 Size: 6607 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3129 3356 928 Size: 971 1230 227 4179 Fragments arranged by size: 4179 1230 971 227 BbvCI CC'TCA_GC Cuts at: 3500 3500 Size: 6607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2928 2962 4798 4832 1903 Size: 34 991 34 1836 34 3678 Fragments arranged by size: 3678 1836 991 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3195 4499 5709 3195 Size: 1304 1210 4093 Fragments arranged by size: 4093 1304 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6607 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5218 136 Size: 107 122 71 631 605 3546 1525 Fragments arranged by size: 3546 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2761 6602 2761 Size: 3841 2766 BlpI GC'TnA_GC Cuts at: 3536 4024 3536 Size: 488 6119 Bme1580I G_kGCm'C Cuts at: 1790 3327 4668 5824 1790 Size: 1537 1341 1156 2573 Fragments arranged by size: 2573 1537 1341 1156 BmgBI CAC'GTC Cuts at: 1318 3598 1318 Size: 2280 4327 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4338 5296 442 Size: 416 53 83 399 24 2921 958 1753 Fragments arranged by size: 2921 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3387 1056 Size: 2331 4276 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3332 3380 5267 1239 Size: 2093 48 1887 2579 Fragments arranged by size: 2579 2093 1887 48 Bpu10I CC'TnA_GC Cuts at: 2132 2999 3245 3500 2132 Size: 867 246 255 5239 Fragments arranged by size: 5239 867 255 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4732 5597 5895 4732 Size: 865 298 5444 Fragments arranged by size: 5444 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3015 5270 882 Size: 617 1516 2255 2219 Fragments arranged by size: 2255 2219 1516 617 BsaAI yAC'GTr Cuts at: 493 1204 3322 3553 4398 493 Size: 711 2118 231 845 2702 Fragments arranged by size: 2702 2118 845 711 231 BsaBI GATnn'nnATC Cuts at: 6601 6601 Size: 6607 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2974 3353 3650 3681 Size: 53 83 186 936 1441 379 297 31 Cuts at: 3681 4414 4796 5431 275 Size: 733 382 635 1451 Fragments arranged by size: 1451 1441 936 733 635 382 379 297 186 83 53 31 BsaWI w'CCGG_w Cuts at: 3416 5040 5438 5712 5859 6270 3416 Size: 1624 398 274 147 411 3753 Fragments arranged by size: 3753 1624 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3311 3341 1178 Size: 30 165 30 1908 30 4444 Fragments arranged by size: 4444 1908 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6607 BseYI C'CCAG_C Cuts at: 3571 5810 6306 3571 Size: 2239 496 3872 Fragments arranged by size: 3872 2239 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2500 2781 3817 1163 Size: 573 764 281 1036 3953 Fragments arranged by size: 3953 1036 764 573 281 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 3393 3403 3630 4057 4818 Size: 275 186 1282 110 10 227 427 761 Cuts at: 4818 5846 1540 Size: 1028 2301 Fragments arranged by size: 2301 1282 1028 761 427 275 227 186 110 10 BsiHKAI G_wGCw'C Cuts at: 3603 3674 3777 4668 4753 5824 3603 Size: 71 103 891 85 1071 4386 Fragments arranged by size: 4386 1071 891 103 85 71 BsmI GAATG_Cn' Cuts at: 2101 2702 4118 4211 2101 Size: 601 1416 93 4497 Fragments arranged by size: 4497 1416 601 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3688 3688 Size: 6607 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 3264 3327 3603 3674 3777 3926 Size: 399 322 1152 63 276 71 103 149 Cuts at: 3926 4668 4753 5824 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1152 1071 742 399 322 276 149 103 85 71 63 BspEI T'CCGG_A Cuts at: 6270 6270 Size: 6607 BspHI T'CATG_A Cuts at: 4496 6226 4496 Size: 1730 4877 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3649 3962 844 Size: 2805 313 3489 Fragments arranged by size: 3489 2805 313 BsrBI CCG'CTC Cuts at: 1394 1823 4494 1394 Size: 429 2671 3507 Fragments arranged by size: 3507 2671 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2131 2376 2721 2912 5102 5276 65 Size: 1183 883 245 345 191 2190 174 1396 Fragments arranged by size: 2190 1396 1183 883 345 245 191 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3113 3629 5251 5438 1700 Size: 110 1303 516 1622 187 2869 Fragments arranged by size: 2869 1622 1303 516 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6607 BssHII G'CGCG_C Cuts at: 1925 3368 1925 Size: 1443 5164 BssSI C'ACGA_G Cuts at: 3263 3478 4667 5679 3263 Size: 215 1189 1012 4191 Fragments arranged by size: 4191 1189 1012 215 BstAPI GCAn_nnn'nTGC Cuts at: 794 3171 3490 3759 794 Size: 2377 319 269 3642 Fragments arranged by size: 3642 2377 319 269 BstBI TT'CG_AA Cuts at: 5411 5411 Size: 6607 BstEII G'GTnAC_C Cuts at: 1184 3412 1184 Size: 2228 4379 BstXI CCAn_nnnn'nTGG Cuts at: 2182 2182 Size: 6607 BstYI r'GATC_y Cuts at: 1972 2338 2457 2761 3937 4304 4689 4706 6158 Size: 366 119 304 1176 367 385 17 1452 Cuts at: 6158 6244 6256 6602 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1176 385 367 366 346 304 119 86 17 12 BstZ17I GTA'TAC Cuts at: 2418 2418 Size: 6607 BtgI C'CryG_G Cuts at: 513 1065 2004 3273 3606 513 Size: 552 939 1269 333 3514 Fragments arranged by size: 3514 1269 939 552 333 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3412 487 Size: 683 56 2186 3682 Fragments arranged by size: 3682 2186 683 56 BtsI GCAGTG_nn' Cuts at: 2293 2488 3520 3672 4123 4917 4937 2293 Size: 195 1032 152 451 794 20 3963 Fragments arranged by size: 3963 1032 794 451 195 152 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6607 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2359 2394 529 Size: 35 1795 35 4742 Fragments arranged by size: 4742 1795 35 35 DraI TTT'AAA Cuts at: 3911 3911 Size: 6607 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5614 809 Size: 507 4298 1802 Fragments arranged by size: 4298 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2367 3210 3280 Size: 54 1425 50 275 18 537 843 70 Cuts at: 3280 3400 3468 3631 3697 4054 4943 6572 8 Size: 120 68 163 66 357 889 1629 43 Fragments arranged by size: 1629 1425 889 843 537 357 275 163 120 70 68 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 3400 4054 1537 Size: 275 1468 120 654 4090 Fragments arranged by size: 4090 1468 654 275 120 EarI CTCTTCn'nnn_ Cuts at: 1353 4537 1353 Size: 3184 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5194 5568 5714 387 Size: 1064 138 3605 374 146 1280 Fragments arranged by size: 3605 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3775 3775 Size: 6607 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2438 3223 3332 3380 3748 4670 5267 Size: 393 806 785 109 48 368 922 597 Cuts at: 5267 6054 6322 1239 Size: 787 268 1524 Fragments arranged by size: 1524 922 806 787 785 597 393 368 268 109 48 EcoRI G'AATT_C Cuts at: 3916 4294 3916 Size: 378 6229 EcoRV GAT'ATC Cuts at: 2047 2350 2047 Size: 303 6304 FspI TGC'GCA Cuts at: 5113 5113 Size: 6607 HaeII r_GCGC'y Cuts at: 1678 3653 3692 5754 1678 Size: 1975 39 2062 2531 Fragments arranged by size: 2531 2062 1975 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2323 2355 2449 2481 2943 Size: 32 135 32 751 32 94 32 462 Cuts at: 2943 2975 3411 3443 5248 5280 5322 5354 1373 Size: 32 436 32 1805 32 42 32 2626 Fragments arranged by size: 2626 1805 751 462 436 135 94 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3951 4197 669 Size: 988 281 2013 246 3079 Fragments arranged by size: 3079 2013 988 281 246 HindIII A'AGCT_T Cuts at: 748 3044 748 Size: 2296 4311 HpaI GTT'AAC Cuts at: 4197 4197 Size: 6607 KasI G'GCGC_C Cuts at: 3649 3649 Size: 6607 KpnI G_GTAC'C Cuts at: 2241 2790 3932 2241 Size: 549 1142 4916 Fragments arranged by size: 4916 1142 549 MfeI C'AATT_G Cuts at: 4206 4206 Size: 6607 MluI A'CGCG_T Cuts at: 6483 6483 Size: 6607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5721 5905 1305 Size: 4416 184 2007 Fragments arranged by size: 4416 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 3212 10 Size: 54 1425 343 537 843 3405 Fragments arranged by size: 3405 1425 843 537 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4565 4924 5083 518 Size: 648 3399 359 159 2042 Fragments arranged by size: 3399 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3275 3571 3655 4023 4700 5848 6093 1606 Size: 1669 296 84 368 677 1148 245 2120 Fragments arranged by size: 2120 1669 1148 677 368 296 245 84 NarI GG'CG_CC Cuts at: 3650 3650 Size: 6607 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5116 Fragments arranged by size: 5116 939 552 NdeI CA'TA_TG Cuts at: 387 2383 387 Size: 1996 4611 NheI G'CTAG_C Cuts at: 1052 3383 1052 Size: 2331 4276 NotI GC'GGCC_GC Cuts at: 3280 4054 3280 Size: 774 5833 NspI r_CATG'y Cuts at: 1132 1229 2524 3158 3965 5510 1132 Size: 97 1295 634 807 1545 2229 Fragments arranged by size: 2229 1545 1295 807 634 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6607 PciI A'CATG_T Cuts at: 5506 5506 Size: 6607 PflMI CCAn_nnn'nTGG Cuts at: 1108 3339 1108 Size: 2231 4376 PmeI GTTT'AAAC Cuts at: 3911 3911 Size: 6607 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4676 4708 6189 6221 4676 Size: 32 1481 32 5062 Fragments arranged by size: 5062 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6607 PsiI TTA'TAA Cuts at: 2471 4177 2471 Size: 1706 4901 PstI C_TGCA'G Cuts at: 830 2761 3176 3660 3959 830 Size: 1931 415 484 299 3478 Fragments arranged by size: 3478 1931 484 415 299 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6607 PvuII CAG'CTG Cuts at: 3571 3571 Size: 6607 SacI G_AGCT'C Cuts at: 3777 3777 Size: 6607 SacII CC_GC'GG Cuts at: 3276 3276 Size: 6607 SalI G'TCGA_C Cuts at: 1936 3949 1936 Size: 2013 4594 SbfI CC_TGCA'GG Cuts at: 3959 3959 Size: 6607 ScaI AGT'ACT Cuts at: 4855 6567 4855 Size: 1712 4895 SexAI A'CCwGG_T Cuts at: 2805 3708 2805 Size: 903 5704 SfcI C'TryA_G Cuts at: 826 1045 2757 3172 3616 3656 3955 5090 5771 Size: 219 1712 415 444 40 299 1135 681 Cuts at: 5771 5962 826 Size: 191 1471 Fragments arranged by size: 1712 1471 1135 681 444 415 299 219 191 40 SfoI GGC'GCC Cuts at: 3651 3651 Size: 6607 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6607 SmaI CCC'GGG Cuts at: 1872 3512 3872 3934 1872 Size: 1640 360 62 4545 Fragments arranged by size: 4545 1640 360 62 SmlI C'TyrA_G Cuts at: 820 1017 1943 4711 5612 5874 820 Size: 197 926 2768 901 262 1553 Fragments arranged by size: 2768 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4398 493 Size: 3905 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6607 SphI G_CATG'C Cuts at: 1132 3158 3965 1132 Size: 2026 807 3774 Fragments arranged by size: 3774 2026 807 SspI AAT'ATT Cuts at: 5 52 2536 2776 4531 5 Size: 47 2484 240 1755 2081 Fragments arranged by size: 2484 2081 1755 240 47 StuI AGG'CCT Cuts at: 1517 2364 2982 1517 Size: 847 618 5142 Fragments arranged by size: 5142 847 618 StyI C'CwwG_G Cuts at: 513 1065 2004 4046 513 Size: 552 939 2042 3074 Fragments arranged by size: 3074 2042 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 4634 4651 4804 4989 5434 325 Size: 1255 446 2608 17 153 185 445 1498 Fragments arranged by size: 2608 1498 1255 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3741 4853 6565 Size: 274 80 33 51 1443 1764 1112 1712 Cuts at: 6565 96 Size: 138 Fragments arranged by size: 1764 1712 1443 1112 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6567 6599 6567 Size: 32 6575 Tth111I GACn'n_nGTC Cuts at: 1316 2228 2807 1316 Size: 912 579 5116 Fragments arranged by size: 5116 912 579 XbaI T'CTAG_A Cuts at: 3943 3943 Size: 6607 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6607 XmaI C'CCGG_G Cuts at: 1870 3510 3870 3932 1870 Size: 1640 360 62 4545 Fragments arranged by size: 4545 1640 360 62 XmnI GAAnn'nnTTC Cuts at: 4736 4736 Size: 6607 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4415 276 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI