(Linear) (Six-base) MAPSORT of: KIEE4174.seq Check: 3208 from: 1 to: 1902 KIEE4174.orf With 182 enzymes: SgfI * March 22, 2012 17:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1644 1902 Size: 1644 258 AccI GT'mk_AC Cuts at: 0 412 1902 Size: 412 1490 Acc65I G'GTAC_C Cuts at: 0 232 781 1902 Size: 232 549 1121 Fragments arranged by size: 1121 549 232 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 1218 1743 1902 Size: 433 785 525 159 Fragments arranged by size: 785 525 433 159 AlwNI CAG_nnn'CTG Cuts at: 0 1485 1902 Size: 1485 417 ApoI r'AATT_y Cuts at: 0 577 601 724 1902 Size: 577 24 123 1178 Fragments arranged by size: 1178 577 123 24 AseI AT'TA_AT Cuts at: 0 647 1902 Size: 647 1255 AvaI C'yCGr_G Cuts at: 0 1505 1765 1801 1856 1865 1902 Size: 1505 260 36 55 9 37 Fragments arranged by size: 1505 260 55 37 36 9 BanI G'GyrC_C Cuts at: 0 3 232 781 1644 1902 Size: 3 229 549 863 258 Fragments arranged by size: 863 549 258 229 3 BanII G_rGCy'C Cuts at: 0 107 1259 1772 1902 Size: 107 1152 513 130 Fragments arranged by size: 1152 513 130 107 BbeI G_GCGC'C Cuts at: 0 1648 1902 Size: 1648 254 BbsI GAAGACnn'nnnn_ Cuts at: 0 1124 1351 1902 Size: 1124 227 551 Fragments arranged by size: 1124 551 227 BbvCI CC'TCA_GC Cuts at: 0 1495 1902 Size: 1495 407 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 923 957 1902 Size: 923 34 945 Fragments arranged by size: 945 923 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1190 1902 Size: 1190 712 BglII A'GATC_T Cuts at: 0 756 1902 Size: 756 1146 BlpI GC'TnA_GC Cuts at: 0 1531 1902 Size: 1531 371 Bme1580I G_kGCm'C Cuts at: 0 1322 1902 Size: 1322 580 BmgBI CAC'GTC Cuts at: 0 1593 1902 Size: 1593 309 BmtI G_CTAG'C Cuts at: 0 1382 1902 Size: 1382 520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1327 1375 1902 Size: 1327 48 527 Fragments arranged by size: 1327 527 48 Bpu10I CC'TnA_GC Cuts at: 0 127 994 1240 1495 1902 Size: 127 867 246 255 407 Fragments arranged by size: 867 407 255 246 127 BsaI GGTCTCn'nnnn_ Cuts at: 0 1010 1902 Size: 1010 892 BsaAI yAC'GTr Cuts at: 0 1317 1548 1902 Size: 1317 231 354 Fragments arranged by size: 1317 354 231 BsaHI Gr'CG_yC Cuts at: 0 969 1348 1645 1676 1902 Size: 969 379 297 31 226 Fragments arranged by size: 969 379 297 226 31 BsaWI w'CCGG_w Cuts at: 0 1411 1902 Size: 1411 491 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1306 1336 1902 Size: 1306 30 566 Fragments arranged by size: 1306 566 30 BseYI C'CCAG_C Cuts at: 0 1566 1902 Size: 1566 336 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 495 776 1812 1902 Size: 495 281 1036 90 Fragments arranged by size: 1036 495 281 90 BsiEI CG_ry'CG Cuts at: 0 1278 1388 1398 1625 1902 Size: 1278 110 10 227 277 Fragments arranged by size: 1278 277 227 110 10 BsiHKAI G_wGCw'C Cuts at: 0 1598 1669 1772 1902 Size: 1598 71 103 130 Fragments arranged by size: 1598 130 103 71 BsmI GAATG_Cn' Cuts at: 0 96 697 1902 Size: 96 601 1205 Fragments arranged by size: 1205 601 96 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1683 1902 Size: 1683 219 Bsp1286I G_dGCh'C Cuts at: 0 107 1259 1322 1598 1669 1772 1902 Size: 107 1152 63 276 71 103 130 Fragments arranged by size: 1152 276 130 107 103 71 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1644 1902 Size: 1644 258 BsrDI GCAATG_nn' Cuts at: 0 126 371 716 907 1902 Size: 126 245 345 191 995 Fragments arranged by size: 995 345 245 191 126 BsrFI r'CCGG_y Cuts at: 0 1108 1624 1902 Size: 1108 516 278 Fragments arranged by size: 1108 516 278 BssHII G'CGCG_C Cuts at: 0 1363 1902 Size: 1363 539 BssSI C'ACGA_G Cuts at: 0 1258 1473 1902 Size: 1258 215 429 Fragments arranged by size: 1258 429 215 BstAPI GCAn_nnn'nTGC Cuts at: 0 1166 1485 1754 1902 Size: 1166 319 269 148 Fragments arranged by size: 1166 319 269 148 BstEII G'GTnAC_C Cuts at: 0 1407 1902 Size: 1407 495 BstXI CCAn_nnnn'nTGG Cuts at: 0 177 1902 Size: 177 1725 BstYI r'GATC_y Cuts at: 0 333 452 756 1902 Size: 333 119 304 1146 Fragments arranged by size: 1146 333 304 119 BstZ17I GTA'TAC Cuts at: 0 413 1902 Size: 413 1489 BtgI C'CryG_G Cuts at: 0 1268 1601 1902 Size: 1268 333 301 Fragments arranged by size: 1268 333 301 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1407 1902 Size: 1407 495 BtsI GCAGTG_nn' Cuts at: 0 288 483 1515 1667 1902 Size: 288 195 1032 152 235 Fragments arranged by size: 1032 288 235 195 152 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 354 389 1902 Size: 354 35 1513 Fragments arranged by size: 1513 354 35 EaeI y'GGCC_r Cuts at: 0 362 1205 1275 1395 1463 1626 1692 1902 Size: 362 843 70 120 68 163 66 210 Fragments arranged by size: 843 362 210 163 120 70 68 66 EagI C'GGCC_G Cuts at: 0 1275 1395 1902 Size: 1275 120 507 Fragments arranged by size: 1275 507 120 EcoICRI GAG'CTC Cuts at: 0 1770 1902 Size: 1770 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 1218 1327 1375 1743 1902 Size: 433 785 109 48 368 159 Fragments arranged by size: 785 433 368 159 109 48 EcoRV GAT'ATC Cuts at: 0 42 345 1902 Size: 42 303 1557 Fragments arranged by size: 1557 303 42 HaeII r_GCGC'y Cuts at: 0 1648 1687 1902 Size: 1648 39 215 Fragments arranged by size: 1648 215 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 318 350 444 476 938 970 1406 1438 Size: 318 32 94 32 462 32 436 32 Cuts at: 1438 1902 Size: 464 Fragments arranged by size: 464 462 436 318 94 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1039 1902 Size: 1039 863 KasI G'GCGC_C Cuts at: 0 1644 1902 Size: 1644 258 KpnI G_GTAC'C Cuts at: 0 236 785 1902 Size: 236 549 1117 Fragments arranged by size: 1117 549 236 MscI TGG'CCA Cuts at: 0 364 1207 1902 Size: 364 843 695 Fragments arranged by size: 843 695 364 MspA1I CmG'CkG Cuts at: 0 1270 1566 1650 1902 Size: 1270 296 84 252 Fragments arranged by size: 1270 296 252 84 NarI GG'CG_CC Cuts at: 0 1645 1902 Size: 1645 257 NdeI CA'TA_TG Cuts at: 0 378 1902 Size: 378 1524 NheI G'CTAG_C Cuts at: 0 1378 1902 Size: 1378 524 NotI GC'GGCC_GC Cuts at: 0 1275 1902 Size: 1275 627 NspI r_CATG'y Cuts at: 0 519 1153 1902 Size: 519 634 749 Fragments arranged by size: 749 634 519 PflMI CCAn_nnn'nTGG Cuts at: 0 1334 1902 Size: 1334 568 PsiI TTA'TAA Cuts at: 0 466 1902 Size: 466 1436 PstI C_TGCA'G Cuts at: 0 756 1171 1655 1902 Size: 756 415 484 247 Fragments arranged by size: 756 484 415 247 PvuII CAG'CTG Cuts at: 0 1566 1902 Size: 1566 336 SacI G_AGCT'C Cuts at: 0 1772 1902 Size: 1772 130 SacII CC_GC'GG Cuts at: 0 1271 1902 Size: 1271 631 SexAI A'CCwGG_T Cuts at: 0 800 1703 1902 Size: 800 903 199 Fragments arranged by size: 903 800 199 SfcI C'TryA_G Cuts at: 0 752 1167 1611 1651 1902 Size: 752 415 444 40 251 Fragments arranged by size: 752 444 415 251 40 SfoI GGC'GCC Cuts at: 0 1646 1902 Size: 1646 256 SmaI CCC'GGG Cuts at: 0 1507 1867 1902 Size: 1507 360 35 Fragments arranged by size: 1507 360 35 SphI G_CATG'C Cuts at: 0 1153 1902 Size: 1153 749 SspI AAT'ATT Cuts at: 0 531 771 1902 Size: 531 240 1131 Fragments arranged by size: 1131 531 240 StuI AGG'CCT Cuts at: 0 359 977 1902 Size: 359 618 925 Fragments arranged by size: 925 618 359 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 21 1902 Size: 21 1881 TatI w'GTAC_w Cuts at: 0 1736 1902 Size: 1736 166 Tth111I GACn'n_nGTC Cuts at: 0 223 802 1902 Size: 223 579 1100 Fragments arranged by size: 1100 579 223 XmaI C'CCGG_G Cuts at: 0 1505 1865 1902 Size: 1505 360 37 Fragments arranged by size: 1505 360 37 Enzymes that do cut: AarI AccI Acc65I AcuI AlwNI ApoI AseI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglII BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI EaeI EagI EcoICRI Eco57MI EcoRV HaeII Hin4I HindIII KasI KpnI MscI MspA1I NarI NdeI NheI NotI NspI PflMI PsiI PstI PvuII SacI SacII SexAI SfcI SfoI SmaI SphI SspI StuI TaqII TatI Tth111I XmaI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BclI BfrBI BglI BmrI BplI BpuEI BsaBI BseRI BsiWI BspEI BspHI BsrBI BsrGI BstBI Bsu36I ClaI DraI DraIII DrdI EarI EciI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HincII HpaI MfeI MluI MmeI MslI NaeI NcoI NgoMIV NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI StyI SwaI TstI XbaI XcmI XhoI XmnI ZraI