Restriction map for
pFN21AE3890
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AccI 1375 GTAGAC GT^[AC][GT]AC
BanII 1760 GAGCCC G[GA]GC[CT]^C
BcnI 1504 CCGGG CC^[GC]GG
BglII 1207 AGATCT A^GATCT
BmyI 1760 GAGCCC G[AGT]GC[ACT]^C
BsmBI 178 GAGACG CGTCTC(1/5)
Bsp1286I 1760 GAGCCC G[AGT]GC[ACT]^C
BspHI 519 TCATGA T^CATGA
BstYI 1207 AGATCT [GA]^GATC[CT]
Cac8I 916 GCTGGC GC[ACGT]^[ACGT]GC
HapII 1504 CCGG C^CGG
HgaI 330 GCGTC GACGC(5/10)
HincII 4 GTTGAC GT[CT]^[GA]AC
HindII 4 GTTGAC GT[CT]^[GA]AC
HpaII 1504 CCGG C^CGG
MflI 1207 AGATCT [GA]^GATC[CT]
MspI 1504 CCGG C^CGG
MwoI 1663 GCTTCTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1504 CCGGG CC^[GC]GG
NspI 1615 ACATGC [GA]CATG^[CT]
PleI 1692 GACTC GAGTC(4/5)
PstI 1067 CTGCAG CTGCA^G
RcaI 519 TCATGA T^CATGA
Tsp45I 744 GTCAC ^GT[GC]AC
XbaI 1872 TCTAGA T^CTAGA
XhoII 1207 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 607 GGATC GGATC(4/5)
1806 GGATC
AlwNI 539 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
742 CAGTCACTG
AseI 409 ATTAAT AT^TAAT
1276 ATTAAT
AsnI 409 ATTAAT AT^TAAT
1276 ATTAAT
AvaII 751 GGACC G^G[AT]CC
1400 GGACC
BsaJI 527 CCCAGG C^C[ACGT][ACGT]GG
1745 CCCTGG
Bsp1407I 36 TGTACA T^GTACA
243 TGTACA
BsrGI 36 TGTACA T^GTACA
243 TGTACA
DraII 750 GGGACCC [GA]G^G[ACGT]CC[CT]
1399 AGGACCT
EcoO109I 750 GGGACCC [GA]G^G[ACGT]CC[CT]
1399 AGGACCT
EcoRI 184 GAATTC G^AATTC
281 GAATTC
HaeIII 557 GGCC GG^CC
1507 GGCC
HphI 511 GGTGA GGTGA(8/7)
565 TCACC
MslI 40 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
223 CATGGGAATG
MspA1I 272 CAGCTG C[AC]G^C[GT]G
1351 CAGCTG
PpuMI 750 GGGACCC [GA]G^G[AT]CC[CT]
1399 AGGACCT
PshBI 409 ATTAAT AT^TAAT
1276 ATTAAT
PvuII 272 CAGCTG CAG^CTG
1351 CAGCTG
SspBI 36 TGTACA T^GTACA
243 TGTACA
SspI 815 AATATT AAT^ATT
1079 AATATT
TaqI 60 TCGA T^CGA
633 TCGA
TthHB8I 60 TCGA T^CGA
633 TCGA
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BbvI BfaI BpmI BpuAI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI