(Circular) (Six-base) MAPSORT of: pFN21AE3890.seq Check: 1912 from: 1 to: 6622 pFN21AE3890 6622 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4432 278 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3381 3965 1937 Size: 1444 584 4594 Fragments arranged by size: 4594 1444 584 Acc65I G'GTAC_C Cuts at: 3943 3943 Size: 6622 AclI AA'CG_TT Cuts at: 1563 4749 5122 1563 Size: 3186 373 3063 Fragments arranged by size: 3186 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3123 3614 4685 6069 6337 1632 Size: 1491 491 1071 1384 268 1917 Fragments arranged by size: 1917 1491 1384 1071 491 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6425 AflIII A'CryG_T Cuts at: 1203 5521 6498 1203 Size: 4318 977 1327 Fragments arranged by size: 4318 1327 977 AgeI A'CCGG_T Cuts at: 5453 5453 Size: 6622 AhdI GACnn_n'nnGTC Cuts at: 5351 5351 Size: 6622 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6622 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6622 AlwNI CAG_nnn'CTG Cuts at: 2549 2752 4365 5937 2549 Size: 203 1613 1572 3234 Fragments arranged by size: 3234 1613 1572 203 ApaLI G'TGCA_C Cuts at: 4679 5835 4679 Size: 1156 5466 ApoI r'AATT_y Cuts at: 1493 2189 2286 2482 2634 3311 3430 3745 3931 Size: 696 97 196 152 677 119 315 186 Cuts at: 3931 4157 4309 6418 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 696 677 315 226 196 186 152 152 119 97 AseI AT'TA_AT Cuts at: 160 1032 2415 3282 5176 160 Size: 872 1383 867 1894 1606 Fragments arranged by size: 1894 1606 1383 872 867 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 AvaI C'yCGr_G Cuts at: 1870 1943 3947 1870 Size: 73 2004 4545 Fragments arranged by size: 4545 2004 73 BamHI G'GATC_C Cuts at: 3952 4319 3952 Size: 367 6255 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3943 5398 618 Size: 325 217 627 2156 1455 1842 Fragments arranged by size: 2156 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3769 3941 1391 Size: 2378 172 4072 Fragments arranged by size: 4072 2378 172 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2330 3585 3866 3909 928 Size: 971 431 1255 281 43 3641 Fragments arranged by size: 3641 1255 971 431 281 43 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4813 4847 1903 Size: 34 2876 34 3678 Fragments arranged by size: 3678 2876 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4514 5724 4514 Size: 1210 5412 BclI T'GATC_A Cuts at: 1548 1548 Size: 6622 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5233 136 Size: 107 122 71 631 605 3561 1525 Fragments arranged by size: 3561 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3212 6617 3212 Size: 3405 3217 BlpI GC'TnA_GC Cuts at: 4039 4039 Size: 6622 Bme1580I G_kGCm'C Cuts at: 1790 4683 5839 1790 Size: 2893 1156 2573 Fragments arranged by size: 2893 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6622 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2762 4353 5311 Size: 416 53 83 399 24 1345 1591 958 Cuts at: 5311 442 Size: 1753 Fragments arranged by size: 1753 1591 1345 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6622 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2301 2405 2934 3030 5282 1239 Size: 1062 104 529 96 2252 2579 Fragments arranged by size: 2579 2252 1062 529 104 96 Bpu10I CC'TnA_GC Cuts at: 3039 3039 Size: 6622 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4747 5612 5910 4747 Size: 865 298 5459 Fragments arranged by size: 5459 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5285 882 Size: 617 3786 2219 Fragments arranged by size: 3786 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4413 493 Size: 711 3209 2702 Fragments arranged by size: 3209 2702 711 BsaBI GATnn'nnATC Cuts at: 6616 6616 Size: 6622 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4429 4811 5446 275 Size: 53 83 186 936 2896 382 635 1451 Fragments arranged by size: 2896 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5055 5453 5727 5874 6285 5055 Size: 398 274 147 411 5392 Fragments arranged by size: 5392 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 1178 Size: 30 165 30 693 30 5674 Fragments arranged by size: 5674 693 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6622 BseYI C'CCAG_C Cuts at: 2558 5825 6321 2558 Size: 3267 496 2859 Fragments arranged by size: 3267 2859 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6049 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4072 4833 5861 1540 Size: 275 186 2071 761 1028 2301 Fragments arranged by size: 2301 2071 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4683 4768 5839 4683 Size: 85 1071 5466 Fragments arranged by size: 5466 1071 85 BsmI GAATG_Cn' Cuts at: 4133 4226 4133 Size: 93 6529 BsmBI CGTCTCn'nnnn_ Cuts at: 2177 2177 Size: 6622 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3769 3941 4683 4768 5839 1391 Size: 399 1979 172 742 85 1071 2174 Fragments arranged by size: 2174 1979 1071 742 399 172 85 BspEI T'CCGG_A Cuts at: 6285 6285 Size: 6622 BspHI T'CATG_A Cuts at: 2524 4511 6241 2524 Size: 1987 1730 2905 Fragments arranged by size: 2905 1987 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3977 844 Size: 3133 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4509 1394 Size: 429 2686 3507 Fragments arranged by size: 3507 2686 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5117 5291 65 Size: 1183 3869 174 1396 Fragments arranged by size: 3869 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5266 5453 1700 Size: 110 3456 187 2869 Fragments arranged by size: 3456 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2041 2248 96 Size: 1945 207 4470 Fragments arranged by size: 4470 1945 207 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6622 BssSI C'ACGA_G Cuts at: 4682 5694 4682 Size: 1012 5610 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6622 BstBI TT'CG_AA Cuts at: 5426 5426 Size: 6622 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6622 BstYI r'GATC_y Cuts at: 1972 3212 3952 4319 4704 4721 6173 6259 6271 Size: 1240 740 367 385 17 1452 86 12 Cuts at: 6271 6617 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1240 740 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5131 Fragments arranged by size: 5131 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5883 Fragments arranged by size: 5883 683 56 BtsI GCAGTG_nn' Cuts at: 3471 4138 4932 4952 3471 Size: 667 794 20 5141 Fragments arranged by size: 5141 794 667 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6587 DraI TTT'AAA Cuts at: 2665 2875 3096 3926 2665 Size: 210 221 830 5361 Fragments arranged by size: 5361 830 221 210 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5629 809 Size: 507 4313 1802 Fragments arranged by size: 4313 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4069 4958 6587 Size: 54 1425 50 275 18 2239 889 1629 Cuts at: 6587 8 Size: 43 Fragments arranged by size: 2239 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4069 1537 Size: 275 2257 4090 Fragments arranged by size: 4090 2257 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2134 2415 2709 3849 4552 1353 Size: 781 281 294 1140 703 3423 Fragments arranged by size: 3423 1140 781 703 294 281 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5209 5583 5729 387 Size: 1064 138 3620 374 146 1280 Fragments arranged by size: 3620 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2405 2934 3030 3123 3614 4685 Size: 393 669 104 529 96 93 491 1071 Cuts at: 4685 5282 6069 6337 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1071 787 669 597 529 491 393 268 104 96 93 EcoO109I rG'GnC_Cy Cuts at: 2756 3405 2756 Size: 649 5973 EcoRI G'AATT_C Cuts at: 2189 2286 3931 4309 2189 Size: 97 1645 378 4502 Fragments arranged by size: 4502 1645 378 97 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2882 2882 Size: 6622 FspI TGC'GCA Cuts at: 5128 5128 Size: 6622 HaeII r_GCGC'y Cuts at: 1678 5769 1678 Size: 4091 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2064 2096 3104 3136 3314 Size: 32 135 32 492 32 1008 32 178 Cuts at: 3314 3346 3859 3891 5263 5295 5337 5369 1373 Size: 32 513 32 1372 32 42 32 2626 Fragments arranged by size: 2626 1372 1008 513 492 178 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2011 3966 4212 669 Size: 988 281 73 1955 246 3079 Fragments arranged by size: 3079 1955 988 281 246 73 HindIII A'AGCT_T Cuts at: 748 748 Size: 6622 HpaI GTT'AAC Cuts at: 4212 4212 Size: 6622 KpnI G_GTAC'C Cuts at: 3947 3947 Size: 6622 MfeI C'AATT_G Cuts at: 4221 4221 Size: 6622 MluI A'CGCG_T Cuts at: 6498 6498 Size: 6622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2050 3560 5736 5920 1305 Size: 745 1510 2176 184 2007 Fragments arranged by size: 2176 2007 1510 745 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4800 Fragments arranged by size: 4800 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2049 2232 4580 4939 5098 518 Size: 648 883 183 2348 359 159 2042 Fragments arranged by size: 2348 2042 883 648 359 183 159 MspA1I CmG'CkG Cuts at: 1606 2279 3358 4038 4715 5863 6108 1606 Size: 673 1079 680 677 1148 245 2120 Fragments arranged by size: 2120 1148 1079 680 677 673 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5131 Fragments arranged by size: 5131 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6622 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6622 NotI GC'GGCC_GC Cuts at: 4069 4069 Size: 6622 NspI r_CATG'y Cuts at: 1132 1229 3624 3980 5525 1132 Size: 97 2395 356 1545 2229 Fragments arranged by size: 2395 2229 1545 356 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6622 PciI A'CATG_T Cuts at: 5521 5521 Size: 6622 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6622 PmeI GTTT'AAAC Cuts at: 3926 3926 Size: 6622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 6204 6236 4691 Size: 32 1481 32 5077 Fragments arranged by size: 5077 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2756 3405 2756 Size: 649 5973 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6622 PsiI TTA'TAA Cuts at: 4192 4192 Size: 6622 PstI C_TGCA'G Cuts at: 830 3076 3974 830 Size: 2246 898 3478 Fragments arranged by size: 3478 2246 898 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6622 PvuII CAG'CTG Cuts at: 2279 3358 2279 Size: 1079 5543 SalI G'TCGA_C Cuts at: 1936 3964 1936 Size: 2028 4594 SanDI GG'GwC_CC Cuts at: 2756 2756 Size: 6622 SbfI CC_TGCA'GG Cuts at: 3974 3974 Size: 6622 ScaI AGT'ACT Cuts at: 4870 6582 4870 Size: 1712 4910 SfcI C'TryA_G Cuts at: 826 1045 2099 2744 3072 3270 3970 5105 5786 Size: 219 1054 645 328 198 700 1135 681 Cuts at: 5786 5977 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1054 700 681 645 328 219 198 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6622 SmaI CCC'GGG Cuts at: 1872 3949 1872 Size: 2077 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4726 5627 5889 820 Size: 197 926 2783 901 262 1553 Fragments arranged by size: 2783 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4413 493 Size: 3920 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6622 SphI G_CATG'C Cuts at: 1132 3980 1132 Size: 2848 3774 SspI AAT'ATT Cuts at: 5 52 2822 3086 4546 5 Size: 47 2770 264 1460 2081 Fragments arranged by size: 2770 2081 1460 264 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6622 StyI C'CwwG_G Cuts at: 513 1065 2004 4061 513 Size: 552 939 2057 3074 Fragments arranged by size: 3074 2057 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3184 4649 4666 4819 5004 5449 325 Size: 1255 1604 1465 17 153 185 445 1498 Fragments arranged by size: 1604 1498 1465 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2041 2248 2358 Size: 274 80 33 51 1443 64 207 110 Cuts at: 2358 4868 6580 96 Size: 2510 1712 138 Fragments arranged by size: 2510 1712 1443 274 207 138 110 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2096 2128 6582 6614 2096 Size: 32 4454 32 2104 Fragments arranged by size: 4454 2104 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6622 XbaI T'CTAG_A Cuts at: 3877 3958 3877 Size: 81 6541 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6622 XmaI C'CCGG_G Cuts at: 1870 3947 1870 Size: 2077 4545 XmnI GAAnn'nnTTC Cuts at: 4751 4751 Size: 6622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4430 276 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI