################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BcgI BciVI BclI BfaI BfrI BlnI BsaBI BsiWI BsmI BspDI BspLU11I BspMI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I EarI Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Alw44I 1311 GTGCAC G^TGCAC ApaLI 1311 GTGCAC G^TGCAC AspHI 1311 GTGCAC G[AT]GC[AT]^C BalI 232 TGGCCA TGG^CCA BamHI 375 GGATCC G^GATCC BbeI 314 GGCGCC GGCGC^C BbrPI 718 CACGTG CAC^GTG BbsI 601 GAAGAC GAAGAC(2/6) BglI 952 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1469 AGATCT A^GATCT BlpI 729 GCTGAGC GC^T[ACGT]AGC Bpu1102I 729 GCTGAGC GC^T[ACGT]AGC BpuAI 601 GAAGAC GAAGAC(2/6) BsaAI 718 CACGTG [CT]AC^GT[GA] BsaI 1342 GGTCTC GGTCTC(1/5) BsiEI 397 CGGCCG CG[GA][CT]^CG BsiHKAI 1311 GTGCAC G[AT]GC[AT]^C BsmBI 1411 GAGACG CGTCTC(1/5) BsmFI 573 GGGAC GGGAC(10/14) Bsp1407I 903 TGTACA T^GTACA BspHI 804 TCATGA T^CATGA BsrDI 1125 CATTGC GCAATG(2/0) BsrGI 903 TGTACA T^GTACA BssHII 261 GCGCGC G^CGCGC CelII 729 GCTGAGC GC^T[ACGT]AGC DraIII 1362 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 778 GACACGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 397 CGGCCG C^GGCCG EclXI 397 CGGCCG C^GGCCG Eco52I 397 CGGCCG C^GGCCG FseI 398 GGCCGGCC GGCCGG^CC KasI 314 GGCGCC G^GCGCC MaeII 719 ACGT A^CGT MluNI 232 TGGCCA TGG^CCA MscI 232 TGGCCA TGG^CCA NarI 314 GGCGCC GG^CGCC PflMI 1011 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 718 CACGTG CAC^GTG PmlI 718 CACGTG CAC^GTG RcaI 804 TCATGA T^CATGA SfcI 964 CTACAG C^T[GA][CT]AG SfoI 314 GGCGCC GGC^GCC SphI 1298 GCATGC GCATG^C SspBI 903 TGTACA T^GTACA Tsp45I 980 GTGAC ^GT[GC]AC Tsp509I 1160 AATT ^AATT Van91I 1011 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 459 CCAGTACGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 314 GGCGCC G[GA]^CG[CT]C 1413 GACGCC AlwI 376 GATCC GGATC(4/5) 375 GGATC Aor51HI 237 AGCGCT AGC^GCT 1218 AGCGCT BbvCI 736 CCTCAGC CCTCAGC(-5/-2) 685 GCTGAGG BsaHI 314 GGCGCC G[GA]^CG[CT]C 1413 GACGCC BstYI 375 GGATCC [GA]^GATC[CT] 1469 AGATCT DpnII 376 GATC ^GATC 1470 GATC DsaI 304 CCGCGG C^C[GA][CT]GG 410 CCGCGG Eco47III 237 AGCGCT AGC^GCT 1218 AGCGCT EcoNI 1266 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1425 CCTGACTGAGG HgaI 604 GACGC GACGC(5/10) 1413 GACGC Hin1I 314 GGCGCC G[GA]^CG[CT]C 1413 GACGCC HphI 979 GGTGA GGTGA(8/7) 650 TCACC KspI 304 CCGCGG CCGC^GG 410 CCGCGG MaeIII 214 GTAAC ^GT[ACGT]AC 980 GTGAC MboI 376 GATC ^GATC 1470 GATC MflI 375 GGATCC [GA]^GATC[CT] 1469 AGATCT MslI 135 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1185 CATTGATGTG NaeI 265 GCCGGC GCC^GGC 399 GCCGGC NdeII 376 GATC ^GATC 1470 GATC NgoMIV 265 GCCGGC G^CCGGC 399 GCCGGC NspI 416 GCATGT [GA]CATG^[CT] 1298 GCATGC SacII 304 CCGCGG CCGC^GG 410 CCGCGG Sau3AI 376 GATC ^GATC 1470 GATC SfaNI 1291 GATGC GCATC(5/9) 1352 GCATC XhoII 375 GGATCC [GA]^GATC[CT] 1469 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AccIII AciI AfaI AluI AlwNI ApaI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsaWI BseAI BseRI BsgI BsiYI BslI BsmAI BsoBI Bsp1286I BspEI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboII MnlI MroI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PpuMI PvuII RsaI Sau96I ScrFI SmaI TfiI TseI TspRI XmaCI XmaI