(Circular) (Six-base) MAPSORT of: pFN21AE1944.seq Check: 4877 from: 1 to: 6205 pFN21AE1944 6205 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3548 1937 Size: 1611 4594 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2969 3125 3491 4268 5652 5920 1632 Size: 1337 156 366 777 1384 268 1917 Fragments arranged by size: 1917 1384 1337 777 366 268 156 AfeI AGC'GCT Cuts at: 2244 3225 2244 Size: 981 5224 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3341 1184 Size: 2157 4048 AlwNI CAG_nnn'CTG Cuts at: 2749 3271 3430 3948 5520 2749 Size: 522 159 518 1572 3434 Fragments arranged by size: 3434 1572 522 518 159 ApaI G_GGCC'C Cuts at: 2400 2541 2684 2400 Size: 141 143 5921 Fragments arranged by size: 5921 143 141 ApaLI G'TGCA_C Cuts at: 3316 4262 5418 3316 Size: 946 1156 4103 Fragments arranged by size: 4103 1156 946 ApoI r'AATT_y Cuts at: 1493 3514 3740 3892 6001 1493 Size: 2021 226 152 2109 1697 Fragments arranged by size: 2109 2021 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2066 2293 2510 2684 3530 1870 Size: 73 90 33 227 217 174 846 4545 Fragments arranged by size: 4545 846 227 217 174 90 73 33 BamHI G'GATC_C Cuts at: 2380 3535 3902 2380 Size: 1155 367 4683 Fragments arranged by size: 4683 1155 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2319 3206 3284 3526 4981 Size: 325 217 627 532 887 78 242 1455 Cuts at: 4981 618 Size: 1842 Fragments arranged by size: 1842 1455 887 627 532 325 242 217 78 BanII G_rGCy'C Cuts at: 1391 2067 2286 2400 2541 2684 2741 3524 1391 Size: 676 219 114 141 143 57 783 4072 Fragments arranged by size: 4072 783 676 219 143 141 114 57 BbeI G_GCGC'C Cuts at: 2323 2323 Size: 6205 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2613 928 Size: 971 714 4520 Fragments arranged by size: 4520 971 714 BbvCI CC'TCA_GC Cuts at: 2691 2742 2691 Size: 51 6154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4396 4430 1903 Size: 34 2459 34 3678 Fragments arranged by size: 3678 2459 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2963 4816 136 Size: 107 122 71 631 605 1291 1853 1525 Fragments arranged by size: 1853 1525 1291 631 605 122 107 71 BglII A'GATC_T Cuts at: 3474 6200 3474 Size: 2726 3479 BlpI GC'TnA_GC Cuts at: 2735 3622 2735 Size: 887 5318 Bme1580I G_kGCm'C Cuts at: 1790 2400 2541 2684 2941 2968 3034 3209 3289 Size: 610 141 143 257 27 66 175 80 Cuts at: 3289 3320 4266 5422 1790 Size: 31 946 1156 2573 Fragments arranged by size: 2573 1156 946 610 257 175 143 141 80 66 31 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3936 4894 442 Size: 416 53 83 399 24 2519 958 1753 Fragments arranged by size: 2519 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2536 2536 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2393 2511 3077 3260 4865 1239 Size: 1154 118 566 183 1605 2579 Fragments arranged by size: 2579 1605 1154 566 183 118 Bpu10I CC'TnA_GC Cuts at: 2691 2742 2691 Size: 51 6154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4330 5195 5493 4330 Size: 865 298 5042 Fragments arranged by size: 5042 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3353 4868 882 Size: 617 1854 1515 2219 Fragments arranged by size: 2219 1854 1515 617 BsaAI yAC'GTr Cuts at: 493 1204 2725 3996 493 Size: 711 1521 1271 2702 Fragments arranged by size: 2702 1521 1271 711 BsaBI GATnn'nnATC Cuts at: 6199 6199 Size: 6205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2320 3419 4012 4394 Size: 53 83 186 936 787 1099 593 382 Cuts at: 4394 5029 275 Size: 635 1451 Fragments arranged by size: 1451 1099 936 787 635 593 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2081 2102 2165 4638 5036 5310 5457 5868 2081 Size: 21 63 2473 398 274 147 411 2418 Fragments arranged by size: 2473 2418 411 398 274 147 63 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2450 2480 2509 2539 2557 Size: 30 165 30 1047 30 29 30 18 Cuts at: 2557 2587 1178 Size: 30 4796 Fragments arranged by size: 4796 1047 165 30 30 30 30 30 29 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2196 2199 2471 2635 2638 1186 Size: 1010 3 272 164 3 4753 Fragments arranged by size: 4753 1010 272 164 3 3 BseYI C'CCAG_C Cuts at: 2225 2556 2674 2818 5408 5904 2225 Size: 331 118 144 2590 496 2526 Fragments arranged by size: 2590 2526 496 331 144 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2548 3212 3218 3311 1163 Size: 573 812 664 6 93 4057 Fragments arranged by size: 4057 812 664 573 93 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2405 3655 4416 5444 1540 Size: 275 186 404 1250 761 1028 2301 Fragments arranged by size: 2301 1250 1028 761 404 275 186 BsiHKAI G_wGCw'C Cuts at: 3320 4266 4351 5422 3320 Size: 946 85 1071 4103 Fragments arranged by size: 4103 1071 946 85 BsmI GAATG_Cn' Cuts at: 3716 3809 3716 Size: 93 6112 BsmBI CGTCTCn'nnnn_ Cuts at: 3410 3410 Size: 6205 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2286 2400 2541 2684 2741 2941 Size: 399 277 219 114 141 143 57 200 Cuts at: 2941 2968 3034 3209 3289 3320 3524 4266 4351 Size: 27 66 175 80 31 204 742 85 Cuts at: 4351 5422 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 277 219 204 200 175 143 141 114 85 80 66 57 31 27 BspEI T'CCGG_A Cuts at: 2081 2102 2165 5868 2081 Size: 21 63 3703 2418 Fragments arranged by size: 3703 2418 63 21 BspHI T'CATG_A Cuts at: 2809 4094 5824 2809 Size: 1285 1730 3190 Fragments arranged by size: 3190 1730 1285 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3560 844 Size: 2716 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2326 2623 3253 4092 1394 Size: 429 503 297 630 839 3507 Fragments arranged by size: 3507 839 630 503 429 297 BsrDI GCAATG_nn' Cuts at: 65 1248 3129 4700 4874 65 Size: 1183 1881 1571 174 1396 Fragments arranged by size: 1881 1571 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2270 2404 2976 4849 5036 1700 Size: 110 460 134 572 1873 187 2869 Fragments arranged by size: 2869 1873 572 460 187 134 110 BsrGI T'GTAC_A Cuts at: 96 2908 96 Size: 2812 3393 BssHII G'CGCG_C Cuts at: 1925 2266 1925 Size: 341 5864 BssSI C'ACGA_G Cuts at: 4265 5277 4265 Size: 1012 5193 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6205 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstYI r'GATC_y Cuts at: 1972 2380 3474 3535 3902 4287 4304 5756 5842 Size: 408 1094 61 367 385 17 1452 86 Cuts at: 5842 5854 6200 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1094 408 385 367 346 86 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 2309 2415 513 Size: 552 1244 106 4303 Fragments arranged by size: 4303 1244 552 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 2808 3721 4515 4535 2808 Size: 913 794 20 4478 Fragments arranged by size: 4478 913 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DraIII CAC_nnn'GTG Cuts at: 3372 3372 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2789 5212 809 Size: 507 1473 2423 1802 Fragments arranged by size: 2423 1802 1473 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2237 2402 2406 Size: 54 1425 50 275 18 407 165 4 Cuts at: 2406 3053 3340 3652 4541 6170 8 Size: 647 287 312 889 1629 43 Fragments arranged by size: 1629 1425 889 647 407 312 287 275 165 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2402 3652 1537 Size: 275 590 1250 4090 Fragments arranged by size: 4090 1250 590 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4135 1353 Size: 2782 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2380 2468 4792 5166 5312 387 Size: 1064 138 791 88 2324 374 146 1280 Fragments arranged by size: 2324 1280 1064 791 374 146 138 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2393 2511 2969 3077 3125 3260 3491 Size: 393 761 118 458 108 48 135 231 Cuts at: 3491 4268 4865 5652 5920 1239 Size: 777 597 787 268 1524 Fragments arranged by size: 1524 787 777 761 597 458 393 268 231 135 118 108 48 EcoNI CCTnn'n_nnAGG Cuts at: 3275 3434 3275 Size: 159 6046 EcoO109I rG'GnC_Cy Cuts at: 2093 2189 2397 2538 2579 2680 2681 2695 2815 Size: 96 208 141 41 101 1 14 120 Cuts at: 2815 2860 3256 3427 2093 Size: 45 396 171 4871 Fragments arranged by size: 4871 396 208 171 141 120 101 96 45 41 14 1 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 FseI GG_CCGG'CC Cuts at: 2408 2408 Size: 6205 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 2112 2246 2323 2735 3016 3227 5352 1678 Size: 434 134 77 412 281 211 2125 2531 Fragments arranged by size: 2531 2125 434 412 281 211 134 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4846 4878 4920 4952 1373 Size: 32 135 32 3274 32 42 32 2626 Fragments arranged by size: 3274 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3549 3795 669 Size: 988 281 1611 246 3079 Fragments arranged by size: 3079 1611 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KasI G'GCGC_C Cuts at: 2319 2319 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2406 5319 5503 1305 Size: 1101 2913 184 2007 Fragments arranged by size: 2913 2007 1101 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2239 10 Size: 54 1425 343 407 3976 Fragments arranged by size: 3976 1425 407 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 3194 4163 4522 4681 518 Size: 648 978 1050 969 359 159 2042 Fragments arranged by size: 2042 1050 978 969 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2225 2311 2417 2749 2822 3036 3097 3338 Size: 619 86 106 332 73 214 61 241 Cuts at: 3338 3621 4298 5446 5691 1606 Size: 283 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 619 332 283 245 241 214 106 86 73 61 NaeI GCC'GGC Cuts at: 2272 2406 2272 Size: 134 6071 NarI GG'CG_CC Cuts at: 2320 2320 Size: 6205 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5653 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NgoMIV G'CCGG_C Cuts at: 2270 2404 2270 Size: 134 6071 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 2425 3307 3563 5108 1132 Size: 97 1196 882 256 1545 2229 Fragments arranged by size: 2229 1545 1196 882 256 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6205 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 3022 1108 Size: 1914 4291 PfoI T'CCnGG_A Cuts at: 2575 2862 2575 Size: 287 5918 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PmlI CAC'GTG Cuts at: 2725 2725 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2189 2579 2695 2860 3427 2189 Size: 390 116 165 567 4967 Fragments arranged by size: 4967 567 390 165 116 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PspOMI G'GGCC_C Cuts at: 2396 2537 2680 2396 Size: 141 143 5921 Fragments arranged by size: 5921 143 141 PstI C_TGCA'G Cuts at: 830 3557 830 Size: 2727 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 PvuII CAG'CTG Cuts at: 2749 3097 3338 2749 Size: 348 241 5616 Fragments arranged by size: 5616 348 241 SacII CC_GC'GG Cuts at: 2312 2418 2312 Size: 106 6099 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SanDI GG'GwC_CC Cuts at: 2579 2579 Size: 6205 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 2969 3553 4688 5369 5560 826 Size: 219 1924 584 1135 681 191 1471 Fragments arranged by size: 1924 1471 1135 681 584 219 191 SfoI GGC'GCC Cuts at: 2321 2321 Size: 6205 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 2035 2068 2295 3532 1872 Size: 163 33 227 1237 4545 Fragments arranged by size: 4545 1237 227 163 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 4309 5210 5472 820 Size: 197 926 2366 901 262 1553 Fragments arranged by size: 2366 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3307 3563 1132 Size: 2175 256 3774 Fragments arranged by size: 3774 2175 256 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 3644 513 Size: 552 2579 3074 Fragments arranged by size: 3074 2579 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2217 2582 4232 4249 4402 4587 5032 Size: 1255 637 365 1650 17 153 185 445 Cuts at: 5032 325 Size: 1498 Fragments arranged by size: 1650 1498 1255 637 445 365 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2908 4451 6163 Size: 274 80 33 51 1443 931 1543 1712 Cuts at: 6163 96 Size: 138 Fragments arranged by size: 1712 1543 1443 931 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2509 2541 2557 2589 6165 6197 2509 Size: 32 16 32 3576 32 2517 Fragments arranged by size: 3576 2517 32 32 32 16 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2471 2471 Size: 6205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 2033 2066 2293 3530 1870 Size: 163 33 227 1237 4545 Fragments arranged by size: 4545 1237 227 163 33 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI