(Linear) (Six-base) MAPSORT of: KIEE1944.seq Check: 882 from: 1 to: 1500 KIEE1944.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 964 1120 1486 1500 Size: 964 156 366 14 Fragments arranged by size: 964 366 156 14 AfeI AGC'GCT Cuts at: 0 239 1220 1500 Size: 239 981 280 Fragments arranged by size: 981 280 239 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1336 1500 Size: 1336 164 AlwNI CAG_nnn'CTG Cuts at: 0 744 1266 1425 1500 Size: 744 522 159 75 Fragments arranged by size: 744 522 159 75 ApaI G_GGCC'C Cuts at: 0 395 536 679 1500 Size: 395 141 143 821 Fragments arranged by size: 821 395 143 141 ApaLI G'TGCA_C Cuts at: 0 1311 1500 Size: 1311 189 AvaI C'yCGr_G Cuts at: 0 28 61 288 505 679 1500 Size: 28 33 227 217 174 821 Fragments arranged by size: 821 227 217 174 33 28 BamHI G'GATC_C Cuts at: 0 375 1500 Size: 375 1125 BanI G'GyrC_C Cuts at: 0 314 1201 1279 1500 Size: 314 887 78 221 Fragments arranged by size: 887 314 221 78 BanII G_rGCy'C Cuts at: 0 62 281 395 536 679 736 1500 Size: 62 219 114 141 143 57 764 Fragments arranged by size: 764 219 143 141 114 62 57 BbeI G_GCGC'C Cuts at: 0 318 1500 Size: 318 1182 BbsI GAAGACnn'nnnn_ Cuts at: 0 608 1500 Size: 608 892 BbvCI CC'TCA_GC Cuts at: 0 686 737 1500 Size: 686 51 763 Fragments arranged by size: 763 686 51 BglI GCCn_nnn'nGGC Cuts at: 0 958 1500 Size: 958 542 BglII A'GATC_T Cuts at: 0 1469 1500 Size: 1469 31 BlpI GC'TnA_GC Cuts at: 0 730 1500 Size: 730 770 Bme1580I G_kGCm'C Cuts at: 0 395 536 679 936 963 1029 1204 1284 Size: 395 141 143 257 27 66 175 80 Cuts at: 1284 1315 1500 Size: 31 185 Fragments arranged by size: 395 257 185 175 143 141 80 66 31 27 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 531 1500 Size: 531 969 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 506 1072 1255 1500 Size: 388 118 566 183 245 Fragments arranged by size: 566 388 245 183 118 Bpu10I CC'TnA_GC Cuts at: 0 686 737 1500 Size: 686 51 763 Fragments arranged by size: 763 686 51 BsaI GGTCTCn'nnnn_ Cuts at: 0 1348 1500 Size: 1348 152 BsaAI yAC'GTr Cuts at: 0 720 1500 Size: 720 780 BsaHI Gr'CG_yC Cuts at: 0 315 1414 1500 Size: 315 1099 86 Fragments arranged by size: 1099 315 86 BsaWI w'CCGG_w Cuts at: 0 76 97 160 1500 Size: 76 21 63 1340 Fragments arranged by size: 1340 76 63 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 445 475 504 534 552 582 1500 Size: 445 30 29 30 18 30 918 Fragments arranged by size: 918 445 30 30 30 29 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 191 194 466 630 633 1500 Size: 191 3 272 164 3 867 Fragments arranged by size: 867 272 191 164 3 3 BseYI C'CCAG_C Cuts at: 0 220 551 669 813 1500 Size: 220 331 118 144 687 Fragments arranged by size: 687 331 220 144 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 543 1207 1213 1306 1500 Size: 543 664 6 93 194 Fragments arranged by size: 664 543 194 93 6 BsiEI CG_ry'CG Cuts at: 0 400 1500 Size: 400 1100 BsiHKAI G_wGCw'C Cuts at: 0 1315 1500 Size: 1315 185 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1405 1500 Size: 1405 95 Bsp1286I G_dGCh'C Cuts at: 0 62 281 395 536 679 736 936 963 Size: 62 219 114 141 143 57 200 27 Cuts at: 963 1029 1204 1284 1315 1500 Size: 66 175 80 31 185 Fragments arranged by size: 219 200 185 175 143 141 114 80 66 62 57 31 27 BspEI T'CCGG_A Cuts at: 0 76 97 160 1500 Size: 76 21 63 1340 Fragments arranged by size: 1340 76 63 21 BspHI T'CATG_A Cuts at: 0 804 1500 Size: 804 696 BsrBI CCG'CTC Cuts at: 0 321 618 1248 1500 Size: 321 297 630 252 Fragments arranged by size: 630 321 297 252 BsrDI GCAATG_nn' Cuts at: 0 1124 1500 Size: 1124 376 BsrFI r'CCGG_y Cuts at: 0 265 399 971 1500 Size: 265 134 572 529 Fragments arranged by size: 572 529 265 134 BsrGI T'GTAC_A Cuts at: 0 903 1500 Size: 903 597 BssHII G'CGCG_C Cuts at: 0 261 1500 Size: 261 1239 BstYI r'GATC_y Cuts at: 0 375 1469 1500 Size: 375 1094 31 Fragments arranged by size: 1094 375 31 BtgI C'CryG_G Cuts at: 0 304 410 1500 Size: 304 106 1090 Fragments arranged by size: 1090 304 106 BtsI GCAGTG_nn' Cuts at: 0 803 1500 Size: 803 697 DraIII CAC_nnn'GTG Cuts at: 0 1367 1500 Size: 1367 133 DrdI GACnn_nn'nnGTC Cuts at: 0 784 1500 Size: 784 716 EaeI y'GGCC_r Cuts at: 0 232 397 401 1048 1335 1500 Size: 232 165 4 647 287 165 Fragments arranged by size: 647 287 232 165 165 4 EagI C'GGCC_G Cuts at: 0 397 1500 Size: 397 1103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 375 463 1500 Size: 375 88 1037 Fragments arranged by size: 1037 375 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 506 964 1072 1120 1255 1486 1500 Size: 388 118 458 108 48 135 231 14 Fragments arranged by size: 458 388 231 135 118 108 48 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 1270 1429 1500 Size: 1270 159 71 Fragments arranged by size: 1270 159 71 EcoO109I rG'GnC_Cy Cuts at: 0 88 184 392 533 574 675 676 690 Size: 88 96 208 141 41 101 1 14 Cuts at: 690 810 855 1251 1422 1500 Size: 120 45 396 171 78 Fragments arranged by size: 396 208 171 141 120 101 96 88 78 45 41 14 1 FseI GG_CCGG'CC Cuts at: 0 403 1500 Size: 403 1097 HaeII r_GCGC'y Cuts at: 0 107 241 318 730 1011 1222 1500 Size: 107 134 77 412 281 211 278 Fragments arranged by size: 412 281 278 211 134 107 77 KasI G'GCGC_C Cuts at: 0 314 1500 Size: 314 1186 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 401 1500 Size: 401 1099 MscI TGG'CCA Cuts at: 0 234 1500 Size: 234 1266 MslI CAynn'nnrTG Cuts at: 0 139 1189 1500 Size: 139 1050 311 Fragments arranged by size: 1050 311 139 MspA1I CmG'CkG Cuts at: 0 220 306 412 744 817 1031 1092 1333 Size: 220 86 106 332 73 214 61 241 Cuts at: 1333 1500 Size: 167 Fragments arranged by size: 332 241 220 214 167 106 86 73 61 NaeI GCC'GGC Cuts at: 0 267 401 1500 Size: 267 134 1099 Fragments arranged by size: 1099 267 134 NarI GG'CG_CC Cuts at: 0 315 1500 Size: 315 1185 NgoMIV G'CCGG_C Cuts at: 0 265 399 1500 Size: 265 134 1101 Fragments arranged by size: 1101 265 134 NspI r_CATG'y Cuts at: 0 420 1302 1500 Size: 420 882 198 Fragments arranged by size: 882 420 198 PflMI CCAn_nnn'nTGG Cuts at: 0 1017 1500 Size: 1017 483 PfoI T'CCnGG_A Cuts at: 0 570 857 1500 Size: 570 287 643 Fragments arranged by size: 643 570 287 PmlI CAC'GTG Cuts at: 0 720 1500 Size: 720 780 PpuMI rG'GwC_Cy Cuts at: 0 184 574 690 855 1422 1500 Size: 184 390 116 165 567 78 Fragments arranged by size: 567 390 184 165 116 78 PspOMI G'GGCC_C Cuts at: 0 391 532 675 1500 Size: 391 141 143 825 Fragments arranged by size: 825 391 143 141 PvuII CAG'CTG Cuts at: 0 744 1092 1333 1500 Size: 744 348 241 167 Fragments arranged by size: 744 348 241 167 SacII CC_GC'GG Cuts at: 0 307 413 1500 Size: 307 106 1087 Fragments arranged by size: 1087 307 106 SanDI GG'GwC_CC Cuts at: 0 574 1500 Size: 574 926 SfcI C'TryA_G Cuts at: 0 964 1500 Size: 964 536 SfoI GGC'GCC Cuts at: 0 316 1500 Size: 316 1184 SmaI CCC'GGG Cuts at: 0 30 63 290 1500 Size: 30 33 227 1210 Fragments arranged by size: 1210 227 33 30 SphI G_CATG'C Cuts at: 0 1302 1500 Size: 1302 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 212 577 1500 Size: 212 365 923 Fragments arranged by size: 923 365 212 TatI w'GTAC_w Cuts at: 0 903 1500 Size: 903 597 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 504 536 552 584 1500 Size: 504 32 16 32 916 Fragments arranged by size: 916 504 32 32 16 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 466 1500 Size: 466 1034 XmaI C'CCGG_G Cuts at: 0 28 61 288 1500 Size: 28 33 227 1212 Fragments arranged by size: 1212 227 33 28 Enzymes that do cut: AcuI AfeI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstYI BtgI BtsI DraIII DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I FseI HaeII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI PmlI PpuMI PspOMI PvuII SacII SanDI SfcI SfoI SmaI SphI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI BpuEI BsaBI BsiWI BsmI BspMI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EarI EcoICRI EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI XmnI ZraI