################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI AspEI AspI AvrII BbeI BbrPI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI FbaI FseI HaeII Hin1I HindIII HpaI KasI KspI MaeI MaeII MamI MfeI MluI MseI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 199 GGTACC G^GTACC AccIII 881 TCCGGA T^CCGGA Alw44I 211 GTGCAC G^TGCAC ApaI 920 GGGCCC GGGCC^C ApaLI 211 GTGCAC G^TGCAC Asp718I 199 GGTACC G^GTACC AvaI 134 CCCGGG C^[CT]CG[GA]G BamHI 1202 GGATCC G^GATCC BanI 199 GGTACC G^G[CT][GA]CC BbsI 475 GAAGAC GAAGAC(2/6) BbvCI 728 CCTCAGC CCTCAGC(-5/-2) BcgI 119 GCAAGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 385 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 475 GAAGAC GAAGAC(2/6) BsaWI 881 TCCGGA [AT]^CCGG[AT] BseAI 881 TCCGGA T^CCGGA BsmBI 129 CGTCTC CGTCTC(1/5) BsoBI 134 CCCGGG C^[CT]CG[GA]G BspEI 881 TCCGGA T^CCGGA BspMI 762 GCAGGT ACCTGC(4/8) BsrFI 340 ACCGGC [GA]^CCGG[CT] BssSI 1036 CACGAG CACGAG(-5/-1) BstAPI 353 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 340 ACCGGC [GA]^CCGG[CT] EcoNI 63 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1213 GATATC GAT^ATC EcoT22I 677 ATGCAT ATGCA^T HgaI 324 GACGC GACGC(5/10) HincII 1087 GTTGAC GT[CT]^[GA]AC HindII 1087 GTTGAC GT[CT]^[GA]AC KpnI 199 GGTACC GGTAC^C MroI 881 TCCGGA T^CCGGA NdeI 315 CATATG CA^TATG NsiI 677 ATGCAT ATGCA^T PflMI 1162 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 298 GAGTC GAGTC(4/5) PpuMI 655 GGGACCC [GA]G^G[AT]CC[CT] PstI 1207 CTGCAG CTGCA^G SacI 889 GAGCTC GAGCT^C SbfI 1206 CCTGCAGG CCTGCA^GG SfcI 1207 CTGCAG C^T[GA][CT]AG SmaI 134 CCCGGG CCC^GGG Sse8387I 1206 CCTGCAGG CCTGCA^GG TfiI 154 GAATC G^A[AT]TC Van91I 1162 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 134 CCCGGG C^CCGGG XmaI 134 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AlwNI 354 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG 1163 CAGACCCTG Asp700I 174 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 504 GAAGAGCTTC AviII 717 TGCGCA TGC^GCA 746 TGCGCA BalI 167 TGGCCA TGG^CCA 311 TGGCCA BsaI 288 GGTCTC GGTCTC(1/5) 1075 GGTCTC BseRI 925 CTCCTC GAGGAG(10/8) 685 GAGGAG BsmFI 29 GGGAC GGGAC(10/14) 655 GGGAC BsrDI 347 GCAATG GCAATG(2/0) 528 GCAATG BstYI 642 GGATCT [GA]^GATC[CT] 1202 GGATCC DpnII 643 GATC ^GATC 1203 GATC DsaI 517 CCGTGG C^C[GA][CT]GG 1056 CCACGG EarI 425 CTCTTC CTCTTC(1/4) 504 GAAGAG FspI 717 TGCGCA TGC^GCA 746 TGCGCA HinfI 154 GAATC G^A[ACGT]TC 298 GAGTC Ksp632I 425 CTCTTC CTCTTC(1/4) 504 GAAGAG MaeIII 205 GTCAC ^GT[ACGT]AC 615 GTCAC MboI 643 GATC ^GATC 1203 GATC MflI 642 GGATCT [GA]^GATC[CT] 1202 GGATCC MluNI 167 TGGCCA TGG^CCA 311 TGGCCA MscI 167 TGGCCA TGG^CCA 311 TGGCCA MspA1I 49 CAGCTG C[AC]G^C[GT]G 388 CAGCTG NdeII 643 GATC ^GATC 1203 GATC NspI 381 GCATGC [GA]CATG^[CT] 758 GCATGC PvuII 49 CAGCTG CAG^CTG 388 CAGCTG SapI 504 GAAGAGC GCTCTTC(1/4) 424 GCTCTTC Sau3AI 643 GATC ^GATC 1203 GATC ScaI 785 AGTACT AGT^ACT 806 AGTACT SexAI 68 ACCAGGT A^CC[AT]GGT 491 ACCAGGT SphI 381 GCATGC GCATG^C 758 GCATGC Tsp45I 205 GTCAC ^GT[GC]AC 615 GTCAC XhoII 642 GGATCT [GA]^GATC[CT] 1202 GGATCC XmnI 174 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 504 GAAGAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboII MnlI MslI MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI StyI TseI TspRI