(Circular) (Six-base) MAPSORT of: pFN21AE3176.seq Check: 898 from: 1 to: 5935 pFN21AE3176 5935 bp With 182 enzymes: SgfI * March 28, 2012 12:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3745 278 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3278 1937 Size: 1341 4594 Acc65I G'GTAC_C Cuts at: 2204 3256 2204 Size: 1052 4883 AclI AA'CG_TT Cuts at: 1563 4062 4435 1563 Size: 2499 373 3063 Fragments arranged by size: 3063 2499 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 3998 5382 5650 1632 Size: 1040 1326 1384 268 1917 Fragments arranged by size: 1917 1384 1326 1040 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5738 AflIII A'CryG_T Cuts at: 1203 4834 5811 1203 Size: 3631 977 1327 Fragments arranged by size: 3631 1327 977 AgeI A'CCGG_T Cuts at: 4766 4766 Size: 5935 AhdI GACnn_n'nnGTC Cuts at: 4664 4664 Size: 5935 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5935 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2135 2167 2938 2970 2135 Size: 32 771 32 5100 Fragments arranged by size: 5100 771 32 32 AlwNI CAG_nnn'CTG Cuts at: 2364 3173 3678 5250 2364 Size: 809 505 1572 3049 Fragments arranged by size: 3049 1572 809 505 ApaI G_GGCC'C Cuts at: 2929 2929 Size: 5935 ApaLI G'TGCA_C Cuts at: 2216 3992 5148 2216 Size: 1776 1156 3003 Fragments arranged by size: 3003 1776 1156 ApoI r'AATT_y Cuts at: 1493 3244 3470 3622 5731 1493 Size: 1751 226 152 2109 1697 Fragments arranged by size: 2109 1751 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4489 160 Size: 872 3457 1606 Fragments arranged by size: 3457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 2139 3260 1870 Size: 73 196 1121 4545 Fragments arranged by size: 4545 1121 196 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2188 2221 2188 Size: 33 5902 BamHI G'GATC_C Cuts at: 3207 3265 3632 3207 Size: 58 367 5510 Fragments arranged by size: 5510 367 58 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2204 3256 4711 618 Size: 325 217 627 417 1052 1455 1842 Fragments arranged by size: 1842 1455 1052 627 417 325 217 BanII G_rGCy'C Cuts at: 1391 2064 2286 2420 2448 2898 2907 2929 3022 Size: 673 222 134 28 450 9 22 93 Cuts at: 3022 3254 1391 Size: 232 4072 Fragments arranged by size: 4072 673 450 232 222 134 93 28 22 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2487 928 Size: 971 588 4376 Fragments arranged by size: 4376 971 588 BbvCI CC'TCA_GC Cuts at: 2734 2734 Size: 5935 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2113 2147 4126 4160 1903 Size: 34 176 34 1979 34 3678 Fragments arranged by size: 3678 1979 176 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3827 5037 3827 Size: 1210 4725 BclI T'GATC_A Cuts at: 1548 1548 Size: 5935 BfrBI ATG'CAT Cuts at: 2684 2684 Size: 5935 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2396 4546 136 Size: 107 122 71 631 605 724 2150 1525 Fragments arranged by size: 2150 1525 724 631 605 122 107 71 BglII A'GATC_T Cuts at: 5930 5930 Size: 5935 BlpI GC'TnA_GC Cuts at: 3352 3352 Size: 5935 Bme1580I G_kGCm'C Cuts at: 1790 2088 2220 2929 3996 5152 1790 Size: 298 132 709 1067 1156 2573 Fragments arranged by size: 2573 1156 1067 709 298 132 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5935 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2258 2371 3666 Size: 416 53 83 399 24 841 113 1295 Cuts at: 3666 4624 442 Size: 958 1753 Fragments arranged by size: 1753 1295 958 841 416 399 113 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2318 2445 2921 4595 1239 Size: 839 240 127 476 1674 2579 Fragments arranged by size: 2579 1674 839 476 240 127 Bpu10I CC'TnA_GC Cuts at: 2734 2734 Size: 5935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4060 4925 5223 4060 Size: 865 298 4772 Fragments arranged by size: 4772 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2299 3086 4598 882 Size: 617 800 787 1512 2219 Fragments arranged by size: 2219 1512 800 787 617 BsaAI yAC'GTr Cuts at: 493 1204 3726 493 Size: 711 2522 2702 Fragments arranged by size: 2702 2522 711 BsaBI GATnn'nnATC Cuts at: 5929 5929 Size: 5935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3742 4124 4759 275 Size: 53 83 186 936 2209 382 635 1451 Fragments arranged by size: 2209 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2886 4368 4766 5040 5187 5598 2886 Size: 1482 398 274 147 411 3223 Fragments arranged by size: 3223 1482 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2135 2165 2938 2968 1178 Size: 30 165 30 732 30 773 30 4145 Fragments arranged by size: 4145 773 732 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2705 2921 1186 Size: 1519 216 4200 Fragments arranged by size: 4200 1519 216 BseYI C'CCAG_C Cuts at: 2391 5138 5634 2391 Size: 2747 496 2692 Fragments arranged by size: 2747 2692 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 2072 2350 2897 1163 Size: 573 318 18 278 547 4201 Fragments arranged by size: 4201 573 547 318 278 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 4146 5174 1540 Size: 275 186 1384 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 2567 2732 2898 3150 3996 4081 5152 2220 Size: 347 165 166 252 846 85 1071 3003 Fragments arranged by size: 3003 1071 846 347 252 166 165 85 BsmI GAATG_Cn' Cuts at: 3446 3539 3446 Size: 93 5842 BsmBI CGTCTCn'nnnn_ Cuts at: 2140 2140 Size: 5935 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2088 2220 2286 2420 2448 2567 Size: 399 274 24 132 66 134 28 119 Cuts at: 2567 2732 2898 2907 2929 3022 3150 3254 3996 Size: 165 166 9 22 93 128 104 742 Cuts at: 3996 4081 5152 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 274 166 165 134 132 128 119 104 93 85 66 28 24 22 9 BspEI T'CCGG_A Cuts at: 2886 5598 2886 Size: 2712 3223 BspHI T'CATG_A Cuts at: 3824 5554 3824 Size: 1730 4205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2758 3290 844 Size: 1914 532 3489 Fragments arranged by size: 3489 1914 532 BsrBI CCG'CTC Cuts at: 1394 1823 3822 1394 Size: 429 1999 3507 Fragments arranged by size: 3507 1999 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2359 2540 4430 4604 65 Size: 1183 1111 181 1890 174 1396 Fragments arranged by size: 1890 1396 1183 1111 181 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2345 4579 4766 1700 Size: 110 535 2234 187 2869 Fragments arranged by size: 2869 2234 535 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5935 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5935 BssSI C'ACGA_G Cuts at: 3041 3995 5007 3041 Size: 954 1012 3969 Fragments arranged by size: 3969 1012 954 BstAPI GCAn_nnn'nTGC Cuts at: 794 2364 794 Size: 1570 4365 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5935 BstYI r'GATC_y Cuts at: 1972 2647 3207 3265 3632 4017 4034 5486 5572 Size: 675 560 58 367 385 17 1452 86 Cuts at: 5572 5584 5930 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 675 560 385 367 346 86 58 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2522 3061 513 Size: 552 939 518 539 3387 Fragments arranged by size: 3387 939 552 539 518 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2691 487 Size: 683 56 1465 3731 Fragments arranged by size: 3731 1465 683 56 BtsI GCAGTG_nn' Cuts at: 3451 4245 4265 3451 Size: 794 20 5121 Fragments arranged by size: 5121 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5900 DraI TTT'AAA Cuts at: 3239 3239 Size: 5935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4942 809 Size: 507 3626 1802 Fragments arranged by size: 3626 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2316 2381 Size: 54 1425 50 275 18 342 144 65 Cuts at: 2381 2421 3382 4271 5900 8 Size: 40 961 889 1629 43 Fragments arranged by size: 1629 1425 961 889 342 275 144 65 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2436 2504 3865 1353 Size: 1083 68 1361 3423 Fragments arranged by size: 3423 1361 1083 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4522 4896 5042 387 Size: 1064 138 2933 374 146 1280 Fragments arranged by size: 2933 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2896 2896 Size: 5935 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2318 2445 2672 2921 3998 4595 Size: 393 446 240 127 227 249 1077 597 Cuts at: 4595 5382 5650 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1077 787 597 446 393 268 249 240 227 127 EcoNI CCTnn'n_nnAGG Cuts at: 2072 2072 Size: 5935 EcoO109I rG'GnC_Cy Cuts at: 2527 2661 2926 2527 Size: 134 265 5536 Fragments arranged by size: 5536 265 134 EcoRI G'AATT_C Cuts at: 3244 3622 3244 Size: 378 5557 EcoRV GAT'ATC Cuts at: 3220 3220 Size: 5935 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2809 2809 Size: 5935 FspI TGC'GCA Cuts at: 2724 2753 4441 2724 Size: 29 1688 4218 Fragments arranged by size: 4218 1688 29 FspAI rTGC'GCAy Cuts at: 2753 2753 Size: 5935 HaeII r_GCGC'y Cuts at: 1678 5082 1678 Size: 3404 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2669 2701 2838 2870 4576 Size: 32 135 32 1097 32 137 32 1706 Cuts at: 4576 4608 4650 4682 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1706 1097 137 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3094 3279 3525 669 Size: 988 281 1156 185 246 3079 Fragments arranged by size: 3079 1156 988 281 246 185 HindIII A'AGCT_T Cuts at: 748 748 Size: 5935 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KpnI G_GTAC'C Cuts at: 2208 3260 2208 Size: 1052 4883 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 5811 5811 Size: 5935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5049 5233 1305 Size: 3744 184 2007 Fragments arranged by size: 3744 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 2318 10 Size: 54 1425 343 342 144 3627 Fragments arranged by size: 3627 1425 343 342 144 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2093 2637 2676 3101 3893 4252 Size: 648 843 84 544 39 425 792 359 Cuts at: 4252 4411 518 Size: 159 2042 Fragments arranged by size: 2042 843 792 648 544 425 359 159 84 39 MspA1I CmG'CkG Cuts at: 1606 2056 2395 3351 4028 5176 5421 1606 Size: 450 339 956 677 1148 245 2120 Fragments arranged by size: 2120 1148 956 677 450 339 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4444 Fragments arranged by size: 4444 939 552 NdeI CA'TA_TG Cuts at: 387 2321 387 Size: 1934 4001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NsiI A_TGCA'T Cuts at: 2686 2686 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 2390 2767 3293 4838 1132 Size: 97 1161 377 526 1545 2229 Fragments arranged by size: 2229 1545 1161 526 377 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5935 PciI A'CATG_T Cuts at: 4834 4834 Size: 5935 PflMI CCAn_nnn'nTGG Cuts at: 1108 3173 1108 Size: 2065 3870 PfoI T'CCnGG_A Cuts at: 2643 2898 2643 Size: 255 5680 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2135 2167 2938 2970 4004 4036 5517 5549 2135 Size: 32 771 32 1034 32 1481 32 2521 Fragments arranged by size: 2521 1481 1034 771 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2661 2661 Size: 5935 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5935 PsiI TTA'TAA Cuts at: 3505 3505 Size: 5935 PspOMI G'GGCC_C Cuts at: 2925 2925 Size: 5935 PstI C_TGCA'G Cuts at: 830 3216 3287 830 Size: 2386 71 3478 Fragments arranged by size: 3478 2386 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 PvuII CAG'CTG Cuts at: 2056 2395 2056 Size: 339 5596 SacI G_AGCT'C Cuts at: 2898 2898 Size: 5935 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SanDI GG'GwC_CC Cuts at: 2661 2661 Size: 5935 SapI GCTCTTCn'nnn_ Cuts at: 2436 2504 2436 Size: 68 5867 SbfI CC_TGCA'GG Cuts at: 3216 3287 3216 Size: 71 5864 ScaI AGT'ACT Cuts at: 2792 2813 4183 5895 2792 Size: 21 1370 1712 2832 Fragments arranged by size: 2832 1712 1370 21 SexAI A'CCwGG_T Cuts at: 2073 2496 2073 Size: 423 5512 SfcI C'TryA_G Cuts at: 826 1045 3212 3283 4418 5099 5290 826 Size: 219 2167 71 1135 681 191 1471 Fragments arranged by size: 2167 1471 1135 681 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 2141 3262 1872 Size: 269 1121 4545 Fragments arranged by size: 4545 1121 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 4039 4940 5202 820 Size: 197 926 2096 901 262 1553 Fragments arranged by size: 2096 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3726 493 Size: 3233 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 2390 2767 3293 1132 Size: 1258 377 526 3774 Fragments arranged by size: 3774 1258 526 377 SspI AAT'ATT Cuts at: 5 52 3859 5 Size: 47 3807 2081 Fragments arranged by size: 3807 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5935 StyI C'CwwG_G Cuts at: 513 1065 2004 2409 2424 3087 3374 513 Size: 552 939 405 15 663 287 3074 Fragments arranged by size: 3074 939 663 552 405 287 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2422 2735 3962 3979 4132 4317 4762 Size: 1255 842 313 1227 17 153 185 445 Cuts at: 4762 325 Size: 1498 Fragments arranged by size: 1498 1255 1227 842 445 313 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 2625 2790 Size: 274 80 33 51 1443 219 429 165 Cuts at: 2790 2811 4181 5893 96 Size: 21 1370 1712 138 Fragments arranged by size: 1712 1443 1370 429 274 219 165 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5895 5927 5895 Size: 32 5903 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5935 XbaI T'CTAG_A Cuts at: 3271 3271 Size: 5935 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5935 XmaI C'CCGG_G Cuts at: 1870 2139 3260 1870 Size: 269 1121 4545 Fragments arranged by size: 4545 1121 269 XmnI GAAnn'nnTTC Cuts at: 2183 2513 4064 2183 Size: 330 1551 4054 Fragments arranged by size: 4054 1551 330 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3743 276 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI