(Linear) (Six-base) MAPSORT of: KIEE3176.seq Check: 3925 from: 1 to: 1230 KIEE3176.orf With 182 enzymes: SgfI * March 22, 2012 16:18 .. Acc65I G'GTAC_C Cuts at: 0 199 1230 Size: 199 1031 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 667 1230 Size: 667 563 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 130 162 933 965 1230 Size: 130 32 771 32 265 Fragments arranged by size: 771 265 130 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 359 1168 1230 Size: 359 809 62 Fragments arranged by size: 809 359 62 ApaI G_GGCC'C Cuts at: 0 924 1230 Size: 924 306 ApaLI G'TGCA_C Cuts at: 0 211 1230 Size: 211 1019 AvaI C'yCGr_G Cuts at: 0 134 1230 Size: 134 1096 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 183 216 1230 Size: 183 33 1014 Fragments arranged by size: 1014 183 33 BamHI G'GATC_C Cuts at: 0 1202 1230 Size: 1202 28 BanI G'GyrC_C Cuts at: 0 199 1230 Size: 199 1031 BanII G_rGCy'C Cuts at: 0 59 281 415 443 893 902 924 1017 Size: 59 222 134 28 450 9 22 93 Cuts at: 1017 1230 Size: 213 Fragments arranged by size: 450 222 213 134 93 59 28 22 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 482 1230 Size: 482 748 BbvCI CC'TCA_GC Cuts at: 0 729 1230 Size: 729 501 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 108 142 1230 Size: 108 34 1088 Fragments arranged by size: 1088 108 34 BfrBI ATG'CAT Cuts at: 0 679 1230 Size: 679 551 BglI GCCn_nnn'nGGC Cuts at: 0 391 1230 Size: 391 839 Bme1580I G_kGCm'C Cuts at: 0 83 215 924 1230 Size: 83 132 709 306 Fragments arranged by size: 709 306 132 83 BmrI ACTGGGnnnn_n' Cuts at: 0 253 366 1230 Size: 253 113 864 Fragments arranged by size: 864 253 113 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 313 440 916 1230 Size: 73 240 127 476 314 Fragments arranged by size: 476 314 240 127 73 Bpu10I CC'TnA_GC Cuts at: 0 729 1230 Size: 729 501 BsaI GGTCTCn'nnnn_ Cuts at: 0 294 1081 1230 Size: 294 787 149 Fragments arranged by size: 787 294 149 BsaWI w'CCGG_w Cuts at: 0 881 1230 Size: 881 349 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 130 160 933 963 1230 Size: 130 30 773 30 267 Fragments arranged by size: 773 267 130 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 700 916 1230 Size: 700 216 314 Fragments arranged by size: 700 314 216 BseYI C'CCAG_C Cuts at: 0 386 1230 Size: 386 844 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 67 345 892 1230 Size: 49 18 278 547 338 Fragments arranged by size: 547 338 278 49 18 BsiHKAI G_wGCw'C Cuts at: 0 215 562 727 893 1145 1230 Size: 215 347 165 166 252 85 Fragments arranged by size: 347 252 215 166 165 85 BsmBI CGTCTCn'nnnn_ Cuts at: 0 135 1230 Size: 135 1095 Bsp1286I G_dGCh'C Cuts at: 0 59 83 215 281 415 443 562 727 Size: 59 24 132 66 134 28 119 165 Cuts at: 727 893 902 924 1017 1145 1230 Size: 166 9 22 93 128 85 Fragments arranged by size: 166 165 134 132 128 119 93 85 66 59 28 24 22 9 BspEI T'CCGG_A Cuts at: 0 881 1230 Size: 881 349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 753 1230 Size: 753 477 BsrDI GCAATG_nn' Cuts at: 0 354 535 1230 Size: 354 181 695 Fragments arranged by size: 695 354 181 BsrFI r'CCGG_y Cuts at: 0 340 1230 Size: 340 890 BssSI C'ACGA_G Cuts at: 0 1036 1230 Size: 1036 194 BstAPI GCAn_nnn'nTGC Cuts at: 0 359 1230 Size: 359 871 BstYI r'GATC_y Cuts at: 0 642 1202 1230 Size: 642 560 28 Fragments arranged by size: 642 560 28 BtgI C'CryG_G Cuts at: 0 517 1056 1230 Size: 517 539 174 Fragments arranged by size: 539 517 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 686 1230 Size: 686 544 EaeI y'GGCC_r Cuts at: 0 167 311 376 416 1230 Size: 167 144 65 40 814 Fragments arranged by size: 814 167 144 65 40 EarI CTCTTCn'nnn_ Cuts at: 0 431 499 1230 Size: 431 68 731 Fragments arranged by size: 731 431 68 EcoICRI GAG'CTC Cuts at: 0 891 1230 Size: 891 339 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 313 440 667 916 1230 Size: 73 240 127 227 249 314 Fragments arranged by size: 314 249 240 227 127 73 EcoNI CCTnn'n_nnAGG Cuts at: 0 67 1230 Size: 67 1163 EcoO109I rG'GnC_Cy Cuts at: 0 522 656 921 1230 Size: 522 134 265 309 Fragments arranged by size: 522 309 265 134 EcoRV GAT'ATC Cuts at: 0 1215 1230 Size: 1215 15 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 804 1230 Size: 804 426 FspI TGC'GCA Cuts at: 0 719 748 1230 Size: 719 29 482 Fragments arranged by size: 719 482 29 FspAI rTGC'GCAy Cuts at: 0 748 1230 Size: 748 482 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 664 696 833 865 1230 Size: 664 32 137 32 365 Fragments arranged by size: 664 365 137 32 32 HincII GTy'rAC Cuts at: 0 1089 1230 Size: 1089 141 KpnI G_GTAC'C Cuts at: 0 203 1230 Size: 203 1027 MscI TGG'CCA Cuts at: 0 169 313 1230 Size: 169 144 917 Fragments arranged by size: 917 169 144 MslI CAynn'nnrTG Cuts at: 0 88 632 671 1096 1230 Size: 88 544 39 425 134 Fragments arranged by size: 544 425 134 88 39 MspA1I CmG'CkG Cuts at: 0 51 390 1230 Size: 51 339 840 Fragments arranged by size: 840 339 51 NdeI CA'TA_TG Cuts at: 0 316 1230 Size: 316 914 NsiI A_TGCA'T Cuts at: 0 681 1230 Size: 681 549 NspI r_CATG'y Cuts at: 0 385 762 1230 Size: 385 377 468 Fragments arranged by size: 468 385 377 PflMI CCAn_nnn'nTGG Cuts at: 0 1168 1230 Size: 1168 62 PfoI T'CCnGG_A Cuts at: 0 638 893 1230 Size: 638 255 337 Fragments arranged by size: 638 337 255 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 130 162 933 965 1230 Size: 130 32 771 32 265 Fragments arranged by size: 771 265 130 32 32 PpuMI rG'GwC_Cy Cuts at: 0 656 1230 Size: 656 574 PspOMI G'GGCC_C Cuts at: 0 920 1230 Size: 920 310 PstI C_TGCA'G Cuts at: 0 1211 1230 Size: 1211 19 PvuII CAG'CTG Cuts at: 0 51 390 1230 Size: 51 339 840 Fragments arranged by size: 840 339 51 SacI G_AGCT'C Cuts at: 0 893 1230 Size: 893 337 SanDI GG'GwC_CC Cuts at: 0 656 1230 Size: 656 574 SapI GCTCTTCn'nnn_ Cuts at: 0 431 499 1230 Size: 431 68 731 Fragments arranged by size: 731 431 68 SbfI CC_TGCA'GG Cuts at: 0 1211 1230 Size: 1211 19 ScaI AGT'ACT Cuts at: 0 787 808 1230 Size: 787 21 422 Fragments arranged by size: 787 422 21 SexAI A'CCwGG_T Cuts at: 0 68 491 1230 Size: 68 423 739 Fragments arranged by size: 739 423 68 SfcI C'TryA_G Cuts at: 0 1207 1230 Size: 1207 23 SmaI CCC'GGG Cuts at: 0 136 1230 Size: 136 1094 SphI G_CATG'C Cuts at: 0 385 762 1230 Size: 385 377 468 Fragments arranged by size: 468 385 377 StyI C'CwwG_G Cuts at: 0 404 419 1082 1230 Size: 404 15 663 148 Fragments arranged by size: 663 404 148 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 417 730 1230 Size: 417 313 500 Fragments arranged by size: 500 417 313 TatI w'GTAC_w Cuts at: 0 191 620 785 806 1230 Size: 191 429 165 21 424 Fragments arranged by size: 429 424 191 165 21 XmaI C'CCGG_G Cuts at: 0 134 1230 Size: 134 1096 XmnI GAAnn'nnTTC Cuts at: 0 178 508 1230 Size: 178 330 722 Fragments arranged by size: 722 330 178 Enzymes that do cut: Acc65I AcuI AloI AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BfrBI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstAPI BstYI BtgI BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI FspAI Hin4I HincII KpnI MscI MslI MspA1I NdeI NsiI NspI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI ScaI SexAI SfcI SmaI SphI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApoI AscI AseI AsiSI AvrII BbeI BciVI BclI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrBI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI FseI HaeII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI