Restriction map for
pFN21AB9166
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# NO CUT ENZYMES ( 75 enzymes) #
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AccIII AclI AcsI AflII AgeI
Aor51HI ApoI AscI AseI AsnI
Asp700I AviII BbeI BciVI BfrI
BglII BlpI Bpu1102I BsaBI BseAI
BsiWI BsmI Bsp1407I BspEI BspHI
BsrGI Bst1107I BstEII BstPI BstZ17I
CelII CpoI DraI Eco47III EcoO65I
EcoRI EcoT22I FseI FspI HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SbfI SfiI
SfoI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 2338 GGTACC G^GTACC
AflIII 1181 ACATGT A^C[GA][CT]GT
AhdI 2190 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 306 GTGCAC G^TGCAC
ApaLI 306 GTGCAC G^TGCAC
Asp718I 2338 GGTACC G^GTACC
AspEI 2190 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1099 GGATCC G^GATCC
BbvCI 1553 CCTCAGC CCTCAGC(-5/-2)
BsaWI 2509 ACCGGA [AT]^CCGG[AT]
BsiEI 5736 CGGCCG CG[GA][CT]^CG
BspDI 1654 ATCGAT AT^CGAT
BspLU11I 1181 ACATGT A^CATGT
BsrDI 5686 CATTGC GCAATG(2/0)
BssHII 5523 GCGCGC G^CGCGC
BssSI 5609 CTCGTG CACGAG(-5/-1)
BstBI 729 TTCGAA TT^CGAA
ClaI 1654 ATCGAT AT^CGAT
DrdI 832 GACAACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5736 CGGCCG C^GGCCG
Eam1105I 2190 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5736 CGGCCG C^GGCCG
Eco52I 5736 CGGCCG C^GGCCG
EcoRV 1651 GATATC GAT^ATC
KpnI 2338 GGTACC GGTAC^C
MseI 497 TTAA T^TAA
NheI 5400 GCTAGC G^CTAGC
NspV 729 TTCGAA TT^CGAA
SacI 4436 GAGCTC GAGCT^C
SapI 4390 GCTCTTC GCTCTTC(1/4)
ScaI 3770 AGTACT AGT^ACT
SexAI 5144 ACCTGGT A^CC[AT]GGT
SfuI 729 TTCGAA TT^CGAA
SgrAI 5405 CGCCGGCG C[GA]^CCGG[CT]G
Tru9I 497 TTAA T^TAA
Tsp509I 2566 AATT ^AATT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AatII 2686 GACGTC GACGT^C
5413 GACGTC
BalI 515 TGGCCA TGG^CCA
3077 TGGCCA
BbrPI 2788 CACGTG CAC^GTG
4641 CACGTG
BclI 1422 TGATCA T^GATCA
3780 TGATCA
BsaAI 2788 CACGTG [CT]AC^GT[GA]
4641 CACGTG
BsrFI 4499 GCCGGC [GA]^CCGG[CT]
5406 GCCGGC
Cfr10I 4499 GCCGGC [GA]^CCGG[CT]
5406 GCCGGC
FbaI 1422 TGATCA T^GATCA
3780 TGATCA
HaeII 4677 GGCGCT [GA]GCGC^[CT]
5403 AGCGCC
MluNI 515 TGGCCA TGG^CCA
3077 TGGCCA
MscI 515 TGGCCA TGG^CCA
3077 TGGCCA
NaeI 4499 GCCGGC GCC^GGC
5406 GCCGGC
NgoMIV 4499 GCCGGC G^CCGGC
5406 GCCGGC
PflMI 1290 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3069 CCACTCTCTGG
PmaCI 2788 CACGTG CAC^GTG
4641 CACGTG
PmlI 2788 CACGTG CAC^GTG
4641 CACGTG
SalI 2722 GTCGAC G^TCGAC
4418 GTCGAC
SmlI 4531 CTCAAG C^T[CT][GA]AG
4960 CTTGAG
SphI 434 GCATGC GCATG^C
5756 GCATGC
Van91I 1290 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3069 CCACTCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII AvrII
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglI BlnI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI Tth111I TthHB8I XcmI XhoII
XmaCI XmaI