################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbsI BcgI BciVI BfrI BglI BglII BlnI BpmI BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I TspRI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AciI 225 CCGC CCGC(-3/-1) AcyI 304 GACGCC G[GA]^CG[CT]C AflIII 317 ACGTGT A^C[GA][CT]GT BalI 143 TGGCCA TGG^CCA BanII 567 GAGCTC G[GA]GC[CT]^C BbrPI 159 CACGTG CAC^GTG BbvCI 394 CCTCAGC CCTCAGC(-5/-2) BclI 26 TGATCA T^GATCA BcnI 285 CCGGG CC^[GC]GG BfaI 400 CTAG C^TAG BsaHI 304 GACGCC G[GA]^CG[CT]C BseRI 418 CTCCTC GAGGAG(10/8) BsmAI 138 GAGAC GTCTC(1/5) BsrFI 528 GCCGGC [GA]^CCGG[CT] CfoI 544 GCGC GCG^C Cfr10I 528 GCCGGC [GA]^CCGG[CT] DraIII 557 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 464 CCGTGG C^C[GA][CT]GG EcoNI 394 CCTCAGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 26 TGATCA T^GATCA HaeII 543 AGCGCC [GA]GCGC^[CT] HgaI 304 GACGC GACGC(5/10) HhaI 544 GCGC GCG^C Hin1I 304 GACGCC G[GA]^CG[CT]C HinP1I 544 GCGC G^CGC HphI 195 GGTGA GGTGA(8/7) MaeI 400 CTAG C^TAG MaeIII 157 GTCAC ^GT[ACGT]AC MboII 244 TCTTC GAAGA(8/7) MluNI 143 TGGCCA TGG^CCA MscI 143 TGGCCA TGG^CCA MspA1I 523 CAGCTG C[AC]G^C[GT]G MwoI 232 GCCCCACTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 528 GCCGGC GCC^GGC NciI 285 CCGGG CC^[GC]GG NgoMIV 528 GCCGGC G^CCGGC PflMI 556 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 366 GACTC GAGTC(4/5) PmaCI 159 CACGTG CAC^GTG PmlI 159 CACGTG CAC^GTG PvuII 523 CAGCTG CAG^CTG SacI 567 GAGCTC GAGCT^C Tsp45I 157 GTCAC ^GT[GC]AC Van91I 556 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 83 GTAC GT^AC 410 GTAC AspHI 50 GTGCTC G[AT]GC[AT]^C 567 GAGCTC BamHI 43 GGATCC G^GATCC 94 GGATCC BlpI 271 GCTGAGC GC^T[ACGT]AGC 569 GCTCAGC BmyI 50 GTGCTC G[AGT]GC[ACT]^C 567 GAGCTC Bpu1102I 271 GCTGAGC GC^T[ACGT]AGC 569 GCTCAGC BsaAI 159 CACGTG [CT]AC^GT[GA] 316 TACGTG BsgI 277 CTGCAC GTGCAG(16/14) 321 GTGCAG BsiHKAI 50 GTGCTC G[AT]GC[AT]^C 567 GAGCTC Bsp1286I 50 GTGCTC G[AGT]GC[ACT]^C 567 GAGCTC BsrI 141 ACTGG ACTGG(1/-1) 589 CCAGT BstYI 43 GGATCC [GA]^GATC[CT] 94 GGATCC CelII 271 GCTGAGC GC^T[ACGT]AGC 569 GCTCAGC EaeI 143 TGGCCA [CT]^GGCC[GA] 461 TGGCCG EcoT14I 132 CCAAGG C^C[AT][AT]GG 342 CCTTGG HapII 285 CCGG C^CGG 529 CCGG HpaII 285 CCGG C^CGG 529 CCGG MflI 43 GGATCC [GA]^GATC[CT] 94 GGATCC MslI 312 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 554 CACCACCATG MspI 285 CCGG C^CGG 529 CCGG RsaI 83 GTAC GT^AC 410 GTAC SfaNI 9 GATGC GCATC(5/9) 311 GCATC StyI 132 CCAAGG C^C[AT][AT]GG 342 CCTTGG TfiI 78 GATTC G^A[AT]TC 434 GATTC XhoII 43 GGATCC [GA]^GATC[CT] 94 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI AlwI AvaII BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeII MboI MnlI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI