################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FokI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AfaI 305 GTAC GT^AC AhdI 418 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 418 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 540 GAGCCC G[GA]GC[CT]^C BcgI 553 GCAAGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 140 TGATCA T^GATCA BfaI 40 CTAG C^TAG BmyI 540 GAGCCC G[AGT]GC[ACT]^C BsaWI 316 TCCGGT [AT]^CCGG[AT] BsiYI 543 CCCAGTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 543 CCCAGTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 540 GAGCCC G[AGT]GC[ACT]^C BspHI 585 TCATGA T^CATGA BsrBI 495 GAGCGG CCGCTC(-3/-3) BsrFI 84 GCCGGC [GA]^CCGG[CT] BstAPI 19 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 561 TTCGAA TT^CGAA BstXI 395 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 193 GCGC GCG^C Cfr10I 84 GCCGGC [GA]^CCGG[CT] Cfr13I 150 GGCCC G^G[ACGT]CC DpnII 141 GATC ^GATC DraII 149 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 209 TGGCCG [CT]^GGCC[GA] Eam1105I 418 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 537 GAAGAG CTCTTC(1/4) EcoO109I 149 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 518 CCAAGG C^C[AT][AT]GG FauI 497 GCGGG CCCGC(4/6) FbaI 140 TGATCA T^GATCA HhaI 193 GCGC GCG^C HinP1I 193 GCGC G^CGC HindIII 328 AAGCTT A^AGCTT Ksp632I 537 GAAGAG CTCTTC(1/4) MaeI 40 CTAG C^TAG MaeII 227 ACGT A^CGT MboI 141 GATC ^GATC MspA1I 212 CCGCTG C[AC]G^C[GT]G NaeI 84 GCCGGC GCC^GGC NdeII 141 GATC ^GATC NgoMIV 84 GCCGGC G^CCGGC NlaIII 586 CATG CATG^ NspV 561 TTCGAA TT^CGAA PleI 418 GACTC GAGTC(4/5) PstI 17 CTGCAG CTGCA^G RcaI 585 TCATGA T^CATGA RsaI 305 GTAC GT^AC SapI 537 GAAGAGC GCTCTTC(1/4) Sau3AI 141 GATC ^GATC Sau96I 150 GGCCC G^G[ACGT]CC SfaNI 248 GCATC GCATC(5/9) SfcI 17 CTGCAG C^T[GA][CT]AG SfuI 561 TTCGAA TT^CGAA StyI 518 CCAAGG C^C[AT][AT]GG TaqI 562 TCGA T^CGA Tsp509I 572 AATT ^AATT TthHB8I 562 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwNI 20 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 380 CAGTTTCTG BbvI 19 GCAGC GCAGC(8/12) 214 GCTGC BsmFI 416 GGGAC GGGAC(10/14) 258 GTCCC HapII 85 CCGG C^CGG 317 CCGG HpaII 85 CCGG C^CGG 317 CCGG MaeIII 162 GTCAC ^GT[ACGT]AC 426 GTCAC MboII 234 GAAGA GAAGA(8/7) 537 GAAGA MseI 326 TTAA T^TAA 593 TTAA MspI 85 CCGG C^CGG 317 CCGG NlaIV 319 GGTTCC GG[ACGT]^[ACGT]CC 514 GGAGCC SmlI 196 CTCAAG C^T[CT][GA]AG 262 CTCAAG TfiI 284 GAATC G^A[AT]TC 345 GATTC Tru9I 326 TTAA T^TAA 593 TTAA TseI 19 GCAGC G^C[AT]GC 214 GCTGC Tsp45I 162 GTCAC ^GT[GC]AC 426 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AciI AluI BsaJI BsrI BssKI BstNI Cac8I DdeI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MnlI MvaI MwoI ScrFI TspRI