################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HindIII HpaI HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SapI SbfI ScaI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 571 GTCGAC GT^[AC][GT]AC AcyI 463 GACGCC G[GA]^CG[CT]C AluI 668 AGCT AG^CT AvaI 325 CCCGAG C^[CT]CG[GA]G BanI 723 GGCACC G^G[CT][GA]CC BbvCI 649 CCTCAGC CCTCAGC(-5/-2) BfaI 620 CTAG C^TAG BsaHI 463 GACGCC G[GA]^CG[CT]C BsiEI 566 CGGCCG CG[GA][CT]^CG BsoBI 325 CCCGAG C^[CT]CG[GA]G Bsp1407I 23 TGTACA T^GTACA BsrBI 30 GAGCGG CCGCTC(-3/-3) BsrFI 75 GCCGGC [GA]^CCGG[CT] BsrGI 23 TGTACA T^GTACA BsrI 71 ACTGG ACTGG(1/-1) BstXI 360 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 75 GCCGGC [GA]^CCGG[CT] DpnII 739 GATC ^GATC DsaI 257 CCACGG C^C[GA][CT]GG EagI 566 CGGCCG C^GGCCG EarI 435 GAAGAG CTCTTC(1/4) EclXI 566 CGGCCG C^GGCCG Eco52I 566 CGGCCG C^GGCCG HaeII 613 AGCGCC [GA]GCGC^[CT] HgaI 463 GACGC GACGC(5/10) Hin1I 463 GACGCC G[GA]^CG[CT]C HincII 571 GTCGAC GT[CT]^[GA]AC HindII 571 GTCGAC GT[CT]^[GA]AC Ksp632I 435 GAAGAG CTCTTC(1/4) MaeI 620 CTAG C^TAG MboI 739 GATC ^GATC MseI 55 TTAA T^TAA NaeI 75 GCCGGC GCC^GGC NdeII 739 GATC ^GATC NgoMIV 75 GCCGGC G^CCGGC NruI 741 TCGCGA TCG^CGA PshAI 564 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 667 GAGCTC GAGCT^C SalI 571 GTCGAC G^TCGAC Sau3AI 739 GATC ^GATC SspBI 23 TGTACA T^GTACA Tru9I 55 TTAA T^TAA Tsp509I 383 AATT ^AATT ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 24 GTAC GT^AC 174 GTAC AspHI 88 GAGCAC G[AT]GC[AT]^C 667 GAGCTC BanII 322 GAGCCC G[GA]GC[CT]^C 667 GAGCTC BsiHKAI 88 GAGCAC G[AT]GC[AT]^C 667 GAGCTC BsmAI 139 GAGAC GTCTC(1/5) 305 GAGAC BsmBI 139 GAGACG CGTCTC(1/5) 305 GAGACG BssSI 136 CACGAG CACGAG(-5/-1) 592 CTCGTG EcoT14I 313 CCTTGG C^C[AT][AT]GG 479 CCAAGG HinfI 153 GAGTC G^A[ACGT]TC 582 GAGTC MslI 15 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 361 CACGCTGGTG MspA1I 300 CAGCGG C[AC]G^C[GT]G 430 CCGCTG NspI 14 GCATGC [GA]CATG^[CT] 714 GCATGC PleI 153 GAGTC GAGTC(4/5) 582 GAGTC RsaI 24 GTAC GT^AC 174 GTAC SexAI 38 ACCTGGT A^CC[AT]GGT 113 ACCAGGT SphI 14 GCATGC GCATG^C 714 GCATGC StuI 266 AGGCCT AGG^CCT 698 AGGCCT StyI 313 CCTTGG C^C[AT][AT]GG 479 CCAAGG TaqI 245 TCGA T^CGA 572 TCGA TspRI 70 CACTG CA[GC]TG[ACGT][ACGT]^ 411 CACTG TthHB8I 245 TCGA T^CGA 572 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AccII AciI AvaII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MvnI MwoI NciI NlaIII NlaIV PpuMI Sau96I ScrFI TseI