(Circular) (Six-base) MAPSORT of: pFN21AE5232.seq Check: 8150 from: 1 to: 5458 pFN21AE5232 5458 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3268 278 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2577 2801 1937 Size: 640 224 4594 Fragments arranged by size: 4594 640 224 Acc65I G'GTAC_C Cuts at: 2779 2779 Size: 5458 AclI AA'CG_TT Cuts at: 1563 3585 3958 1563 Size: 2022 373 3063 Fragments arranged by size: 3063 2022 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2212 2459 3521 4905 5173 1632 Size: 580 247 1062 1384 268 1917 Fragments arranged by size: 1917 1384 1062 580 268 247 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5261 AflIII A'CryG_T Cuts at: 1203 4357 5334 1203 Size: 3154 977 1327 Fragments arranged by size: 3154 1327 977 AgeI A'CCGG_T Cuts at: 4289 4289 Size: 5458 AhdI GACnn_n'nnGTC Cuts at: 4187 4187 Size: 5458 AleI CACnn'nnGTG Cuts at: 1166 2370 1166 Size: 1204 4254 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5458 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2576 2608 2576 Size: 32 5426 AlwNI CAG_nnn'CTG Cuts at: 3201 4773 3201 Size: 1572 3886 ApaLI G'TGCA_C Cuts at: 3515 4671 3515 Size: 1156 4302 ApoI r'AATT_y Cuts at: 1493 2767 2993 3145 5254 1493 Size: 1274 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 226 152 AseI AT'TA_AT Cuts at: 160 1032 4012 160 Size: 872 2980 1606 Fragments arranged by size: 2980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 AvaI C'yCGr_G Cuts at: 1870 1943 2330 2783 1870 Size: 73 387 453 4545 Fragments arranged by size: 4545 453 387 73 BamHI G'GATC_C Cuts at: 2788 3155 2788 Size: 367 5091 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2728 2779 4234 618 Size: 325 217 627 941 51 1455 1842 Fragments arranged by size: 1842 1455 941 627 325 217 51 BanII G_rGCy'C Cuts at: 1391 2331 2676 2777 1391 Size: 940 345 101 4072 Fragments arranged by size: 4072 940 345 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4487 BbvCI CC'TCA_GC Cuts at: 2655 2655 Size: 5458 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3649 3683 1903 Size: 34 1712 34 3678 Fragments arranged by size: 3678 1712 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3350 4560 3350 Size: 1210 4248 BclI T'GATC_A Cuts at: 1548 1548 Size: 5458 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4069 136 Size: 107 122 71 631 605 337 2060 1525 Fragments arranged by size: 2060 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5453 5453 Size: 5458 BlpI GC'TnA_GC Cuts at: 2875 2875 Size: 5458 Bme1580I G_kGCm'C Cuts at: 1790 3519 4675 1790 Size: 1729 1156 2573 Fragments arranged by size: 2573 1729 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5458 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3189 4147 442 Size: 416 53 83 399 24 1772 958 1753 Fragments arranged by size: 1772 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5458 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2722 2722 Size: 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4118 1239 Size: 2879 2579 Bpu10I CC'TnA_GC Cuts at: 2655 2655 Size: 5458 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3583 4448 4746 3583 Size: 865 298 4295 Fragments arranged by size: 4295 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4121 882 Size: 617 2622 2219 Fragments arranged by size: 2622 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3249 493 Size: 711 2045 2702 Fragments arranged by size: 2702 2045 711 BsaBI GATnn'nnATC Cuts at: 5452 5452 Size: 5458 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2469 3265 3647 4282 Size: 53 83 186 936 936 796 382 635 Cuts at: 4282 275 Size: 1451 Fragments arranged by size: 1451 936 936 796 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3891 4289 4563 4710 5121 3891 Size: 398 274 147 411 4228 Fragments arranged by size: 4228 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2576 2606 1178 Size: 30 165 30 1173 30 4030 Fragments arranged by size: 4030 1173 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 2438 2458 2522 2525 2597 2678 2681 Size: 1249 3 20 64 3 72 81 3 Cuts at: 2681 2684 2714 1186 Size: 3 30 3930 Fragments arranged by size: 3930 1249 81 72 64 30 20 3 3 3 3 BseYI C'CCAG_C Cuts at: 2103 4661 5157 2103 Size: 2558 496 2404 Fragments arranged by size: 2558 2404 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4885 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2574 2908 3669 4697 1540 Size: 275 186 573 334 761 1028 2301 Fragments arranged by size: 2301 1028 761 573 334 275 186 BsiHKAI G_wGCw'C Cuts at: 2097 2676 3519 3604 4675 2097 Size: 579 843 85 1071 2880 Fragments arranged by size: 2880 1071 843 579 85 BsmI GAATG_Cn' Cuts at: 2969 3062 2969 Size: 93 5365 BsmBI CGTCTCn'nnnn_ Cuts at: 2138 2304 2138 Size: 166 5292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2331 2676 2777 3519 3604 4675 Size: 399 307 234 345 101 742 85 1071 Cuts at: 4675 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 345 307 234 101 85 BspEI T'CCGG_A Cuts at: 5121 5121 Size: 5458 BspHI T'CATG_A Cuts at: 3347 5077 3347 Size: 1730 3728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2813 844 Size: 1969 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2037 3345 1394 Size: 429 214 1308 3507 Fragments arranged by size: 3507 1308 429 214 BsrDI GCAATG_nn' Cuts at: 65 1248 3953 4127 65 Size: 1183 2705 174 1396 Fragments arranged by size: 2705 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2080 4102 4289 1700 Size: 110 270 2022 187 2869 Fragments arranged by size: 2869 2022 270 187 110 BsrGI T'GTAC_A Cuts at: 96 2028 96 Size: 1932 3526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5458 BssSI C'ACGA_G Cuts at: 2141 2597 3518 4530 2141 Size: 456 921 1012 3069 Fragments arranged by size: 3069 1012 921 456 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5458 BstBI TT'CG_AA Cuts at: 4262 4262 Size: 5458 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5458 BstXI CCAn_nnnn'nTGG Cuts at: 2372 2372 Size: 5458 BstYI r'GATC_y Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453 Size: 816 367 385 17 1452 86 12 346 Cuts at: 5453 1972 Size: 1977 Fragments arranged by size: 1977 1452 816 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2262 513 Size: 552 1197 3709 Fragments arranged by size: 3709 1197 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4719 Fragments arranged by size: 4719 683 56 BtsI GCAGTG_nn' Cuts at: 2974 3768 3788 2974 Size: 794 20 4644 Fragments arranged by size: 4644 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5458 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5423 DraI TTT'AAA Cuts at: 2762 2762 Size: 5458 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4465 809 Size: 507 3149 1802 Fragments arranged by size: 3149 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2078 2321 2571 Size: 54 1425 50 275 18 248 243 250 Cuts at: 2571 2905 3794 5423 8 Size: 334 889 1629 43 Fragments arranged by size: 1629 1425 889 334 275 250 248 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2571 2905 1537 Size: 275 759 334 4090 Fragments arranged by size: 4090 759 334 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2435 3388 1353 Size: 1082 953 3423 Fragments arranged by size: 3423 1082 953 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2531 2537 2549 2754 4045 4419 Size: 1064 138 942 6 12 205 1291 374 Cuts at: 4419 4565 387 Size: 146 1280 Fragments arranged by size: 1291 1280 1064 942 374 205 146 138 12 6 EcoICRI GAG'CTC Cuts at: 2674 2674 Size: 5458 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2459 3521 4118 4905 5173 1239 Size: 393 580 247 1062 597 787 268 1524 Fragments arranged by size: 1524 1062 787 597 580 393 268 247 EcoO109I rG'GnC_Cy Cuts at: 2041 2136 2455 2041 Size: 95 319 5044 Fragments arranged by size: 5044 319 95 EcoRI G'AATT_C Cuts at: 2767 3145 2767 Size: 378 5080 FspI TGC'GCA Cuts at: 3964 3964 Size: 5458 HaeII r_GCGC'y Cuts at: 1678 2622 4605 1678 Size: 944 1983 2531 Fragments arranged by size: 2531 1983 944 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4099 4131 4173 4205 1373 Size: 32 135 32 2527 32 42 32 2626 Fragments arranged by size: 2626 2527 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2578 2802 3048 669 Size: 988 281 640 224 246 3079 Fragments arranged by size: 3079 988 640 281 246 224 HindIII A'AGCT_T Cuts at: 748 748 Size: 5458 HpaI GTT'AAC Cuts at: 3048 3048 Size: 5458 KpnI G_GTAC'C Cuts at: 2783 2783 Size: 5458 MfeI C'AATT_G Cuts at: 3057 3057 Size: 5458 MluI A'CGCG_T Cuts at: 5334 5334 Size: 5458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 4572 4756 1305 Size: 996 2271 184 2007 Fragments arranged by size: 2271 2007 996 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3636 Fragments arranged by size: 3636 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2370 3416 3775 3934 518 Size: 648 858 346 1046 359 159 2042 Fragments arranged by size: 2042 1046 858 648 359 346 159 MspA1I CmG'CkG Cuts at: 1606 2307 2437 2874 3551 4699 4944 1606 Size: 701 130 437 677 1148 245 2120 Fragments arranged by size: 2120 1148 701 677 437 245 130 NaeI GCC'GGC Cuts at: 2082 2082 Size: 5458 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4906 NdeI CA'TA_TG Cuts at: 387 387 Size: 5458 NgoMIV G'CCGG_C Cuts at: 2080 2080 Size: 5458 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5458 NotI GC'GGCC_GC Cuts at: 2905 2905 Size: 5458 NruI TCG'CGA Cuts at: 2748 2748 Size: 5458 NspI r_CATG'y Cuts at: 1132 1229 2023 2723 2816 4361 1132 Size: 97 794 700 93 1545 2229 Fragments arranged by size: 2229 1545 794 700 97 93 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5458 PciI A'CATG_T Cuts at: 4357 4357 Size: 5458 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5458 PfoI T'CCnGG_A Cuts at: 2676 2676 Size: 5458 PmeI GTTT'AAAC Cuts at: 2762 2762 Size: 5458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2576 2608 3527 3559 5040 5072 2576 Size: 32 919 32 1481 32 2962 Fragments arranged by size: 2962 1481 919 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 2136 2455 2041 Size: 95 319 5044 Fragments arranged by size: 5044 319 95 PshAI GACnn'nnGTC Cuts at: 1613 2573 1613 Size: 960 4498 PsiI TTA'TAA Cuts at: 3028 3028 Size: 5458 PstI C_TGCA'G Cuts at: 830 2810 830 Size: 1980 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5458 SacI G_AGCT'C Cuts at: 2676 2676 Size: 5458 SalI G'TCGA_C Cuts at: 1936 2576 2800 1936 Size: 640 224 4594 Fragments arranged by size: 4594 640 224 SanDI GG'GwC_CC Cuts at: 2136 2455 2136 Size: 319 5139 SbfI CC_TGCA'GG Cuts at: 2810 2810 Size: 5458 ScaI AGT'ACT Cuts at: 3706 5418 3706 Size: 1712 3746 SexAI A'CCwGG_T Cuts at: 2043 2118 2043 Size: 75 5383 SfcI C'TryA_G Cuts at: 826 1045 2806 3941 4622 4813 826 Size: 219 1761 1135 681 191 1471 Fragments arranged by size: 1761 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5458 SmaI CCC'GGG Cuts at: 1872 2785 1872 Size: 913 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3562 4463 4725 820 Size: 197 926 1619 901 262 1553 Fragments arranged by size: 1619 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3249 493 Size: 2756 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5458 SphI G_CATG'C Cuts at: 1132 2023 2723 2816 1132 Size: 891 700 93 3774 Fragments arranged by size: 3774 891 700 93 SspI AAT'ATT Cuts at: 5 52 3382 5 Size: 47 3330 2081 Fragments arranged by size: 3330 2081 47 StuI AGG'CCT Cuts at: 1517 2273 2705 1517 Size: 756 432 4270 Fragments arranged by size: 4270 756 432 StyI C'CwwG_G Cuts at: 513 1065 2318 2484 2897 513 Size: 552 1253 166 413 3074 Fragments arranged by size: 3074 1253 552 413 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3485 3502 3655 3840 4285 325 Size: 1255 1905 17 153 185 445 1498 Fragments arranged by size: 1905 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2028 3704 5416 Size: 274 80 33 51 1443 51 1676 1712 Cuts at: 5416 96 Size: 138 Fragments arranged by size: 1712 1676 1443 274 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5418 5450 5418 Size: 32 5426 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5458 XbaI T'CTAG_A Cuts at: 2794 2794 Size: 5458 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5458 XmaI C'CCGG_G Cuts at: 1870 2783 1870 Size: 913 4545 XmnI GAAnn'nnTTC Cuts at: 3587 3587 Size: 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3266 276 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI