(Linear) (Six-base) MAPSORT of: KIEE5232.seq Check: 4584 from: 1 to: 753 KIEE5232.orf With 182 enzymes: SgfI * January 29, 2014 11:52 .. AccI GT'mk_AC Cuts at: 0 572 753 Size: 572 181 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 454 753 Size: 207 247 299 Fragments arranged by size: 299 247 207 AleI CACnn'nnGTG Cuts at: 0 365 753 Size: 365 388 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 571 603 753 Size: 571 32 150 Fragments arranged by size: 571 150 32 AvaI C'yCGr_G Cuts at: 0 325 753 Size: 325 428 BanI G'GyrC_C Cuts at: 0 723 753 Size: 723 30 BanII G_rGCy'C Cuts at: 0 326 671 753 Size: 326 345 82 Fragments arranged by size: 345 326 82 BbvCI CC'TCA_GC Cuts at: 0 650 753 Size: 650 103 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 717 753 Size: 717 36 Bpu10I CC'TnA_GC Cuts at: 0 650 753 Size: 650 103 BsaHI Gr'CG_yC Cuts at: 0 464 753 Size: 464 289 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 571 601 753 Size: 571 30 152 Fragments arranged by size: 571 152 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 430 433 453 517 520 592 673 676 Size: 430 3 20 64 3 72 81 3 Cuts at: 676 679 709 753 Size: 3 30 44 Fragments arranged by size: 430 81 72 64 44 30 20 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 98 753 Size: 98 655 BsiEI CG_ry'CG Cuts at: 0 569 753 Size: 569 184 BsiHKAI G_wGCw'C Cuts at: 0 92 671 753 Size: 92 579 82 Fragments arranged by size: 579 92 82 BsmBI CGTCTCn'nnnn_ Cuts at: 0 133 299 753 Size: 133 166 454 Fragments arranged by size: 454 166 133 Bsp1286I G_dGCh'C Cuts at: 0 92 326 671 753 Size: 92 234 345 82 Fragments arranged by size: 345 234 92 82 BsrBI CCG'CTC Cuts at: 0 32 753 Size: 32 721 BsrFI r'CCGG_y Cuts at: 0 75 753 Size: 75 678 BsrGI T'GTAC_A Cuts at: 0 23 753 Size: 23 730 BssSI C'ACGA_G Cuts at: 0 136 592 753 Size: 136 456 161 Fragments arranged by size: 456 161 136 BstXI CCAn_nnnn'nTGG Cuts at: 0 367 753 Size: 367 386 BtgI C'CryG_G Cuts at: 0 257 753 Size: 257 496 EaeI y'GGCC_r Cuts at: 0 73 316 566 753 Size: 73 243 250 187 Fragments arranged by size: 250 243 187 73 EagI C'GGCC_G Cuts at: 0 566 753 Size: 566 187 EarI CTCTTCn'nnn_ Cuts at: 0 430 753 Size: 430 323 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 526 532 544 749 753 Size: 526 6 12 205 4 Fragments arranged by size: 526 205 12 6 4 EcoICRI GAG'CTC Cuts at: 0 669 753 Size: 669 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 454 753 Size: 207 247 299 Fragments arranged by size: 299 247 207 EcoO109I rG'GnC_Cy Cuts at: 0 36 131 450 753 Size: 36 95 319 303 Fragments arranged by size: 319 303 95 36 HaeII r_GCGC'y Cuts at: 0 617 753 Size: 617 136 HincII GTy'rAC Cuts at: 0 573 753 Size: 573 180 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 296 753 Size: 296 457 MslI CAynn'nnrTG Cuts at: 0 19 365 753 Size: 19 346 388 Fragments arranged by size: 388 346 19 MspA1I CmG'CkG Cuts at: 0 302 432 753 Size: 302 130 321 Fragments arranged by size: 321 302 130 NaeI GCC'GGC Cuts at: 0 77 753 Size: 77 676 NgoMIV G'CCGG_C Cuts at: 0 75 753 Size: 75 678 NruI TCG'CGA Cuts at: 0 743 753 Size: 743 10 NspI r_CATG'y Cuts at: 0 18 718 753 Size: 18 700 35 Fragments arranged by size: 700 35 18 PfoI T'CCnGG_A Cuts at: 0 671 753 Size: 671 82 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 571 603 753 Size: 571 32 150 Fragments arranged by size: 571 150 32 PpuMI rG'GwC_Cy Cuts at: 0 36 131 450 753 Size: 36 95 319 303 Fragments arranged by size: 319 303 95 36 PshAI GACnn'nnGTC Cuts at: 0 568 753 Size: 568 185 SacI G_AGCT'C Cuts at: 0 671 753 Size: 671 82 SalI G'TCGA_C Cuts at: 0 571 753 Size: 571 182 SanDI GG'GwC_CC Cuts at: 0 131 450 753 Size: 131 319 303 Fragments arranged by size: 319 303 131 SexAI A'CCwGG_T Cuts at: 0 38 113 753 Size: 38 75 640 Fragments arranged by size: 640 75 38 SphI G_CATG'C Cuts at: 0 18 718 753 Size: 18 700 35 Fragments arranged by size: 700 35 18 StuI AGG'CCT Cuts at: 0 268 700 753 Size: 268 432 53 Fragments arranged by size: 432 268 53 StyI C'CwwG_G Cuts at: 0 313 479 753 Size: 313 166 274 Fragments arranged by size: 313 274 166 TatI w'GTAC_w Cuts at: 0 23 753 Size: 23 730 Enzymes that do cut: AccI AcuI AleI AloI AvaI BanI BanII BbvCI BplI Bpu10I BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BsrGI BssSI BstXI BtgI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII HincII MmeI MslI MspA1I NaeI NgoMIV NruI NspI PfoI PpiI PpuMI PshAI SacI SalI SanDI SexAI SphI StuI StyI TatI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI BspMI BsrDI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NdeI NheI NotI NsiI PacI PasI PciI PflMI PmeI PmlI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI