################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BclI BfaI BfrI BglII BlnI BsaBI BsaI BsmAI BsmBI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 1983 TCCGGA T^CCGGA AflIII 1559 ACACGT A^C[GA][CT]GT AgeI 1268 ACCGGT A^CCGGT AspHI 1903 GAGCAC G[AT]GC[AT]^C BamHI 2103 GGATCC G^GATCC BanI 2325 GGCGCC G^G[CT][GA]CC BbeI 2325 GGCGCC GGCGC^C BbrPI 1078 CACGTG CAC^GTG BciVI 1941 GTATCC GTATCC(6/5) BlpI 583 GCTGAGC GC^T[ACGT]AGC Bpu1102I 583 GCTGAGC GC^T[ACGT]AGC BsaAI 1078 CACGTG [CT]AC^GT[GA] BseAI 1983 TCCGGA T^CCGGA BsiHKAI 1903 GAGCAC G[AT]GC[AT]^C BsmI 28 GAATGC GAATGC(1/-1) BspEI 1983 TCCGGA T^CCGGA BspHI 1721 TCATGA T^CATGA BstAPI 23 GCAACGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1614 GGTCACC G^GT[ACGT]ACC BstPI 1614 GGTCACC G^GT[ACGT]ACC CelII 583 GCTGAGC GC^T[ACGT]AGC DrdI 336 GACCCCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1029 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1614 GGTCACC G^GT[ACGT]ACC KasI 2325 GGCGCC G^GCGCC KspI 2297 CCGCGG CCGC^GG MroI 1983 TCCGGA T^CCGGA NarI 2325 GGCGCC GG^CGCC NcoI 1637 CCATGG C^CATGG PinAI 1268 ACCGGT A^CCGGT PmaCI 1078 CACGTG CAC^GTG PmlI 1078 CACGTG CAC^GTG RcaI 1721 TCATGA T^CATGA SacII 2297 CCGCGG CCGC^GG SapI 987 GAAGAGC GCTCTTC(1/4) SexAI 1442 ACCTGGT A^CC[AT]GGT SfoI 2325 GGCGCC GGC^GCC SmlI 2059 CTCAAG C^T[CT][GA]AG TaqI 1829 TCGA T^CGA TfiI 2186 GAATC G^A[AT]TC Tsp509I 2394 AATT ^AATT TthHB8I 1829 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 567 GACGCC G[GA]^CG[CT]C 2325 GGCGCC AlwNI 1438 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 1462 CAGCCCCTG AocI 1603 CCTGAGG CC^T[ACGT]AGG 2398 CCTCAGG Aor51HI 271 AGCGCT AGC^GCT 509 AGCGCT ApaI 850 GGGCCC GGGCC^C 1004 GGGCCC BbvCI 678 CCTCAGC CCTCAGC(-5/-2) 1013 CCTCAGC BcgI 187 GCAGGGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 472 GCAGTGGCCTCG BsaHI 567 GACGCC G[GA]^CG[CT]C 2325 GGCGCC BsaWI 1268 ACCGGT [AT]^CCGG[AT] 1983 TCCGGA BsiWI 314 CGTACG C^GTACG 1064 CGTACG BspMI 365 ACCTGC ACCTGC(4/8) 1658 ACCTGC BsrBI 16 GAGCGG CCGCTC(-3/-3) 1395 GAGCGG Bsu36I 1603 CCTGAGG CC^T[ACGT]AGG 2398 CCTCAGG EagI 482 CGGCCG C^GGCCG 771 CGGCCG EclXI 482 CGGCCG C^GGCCG 771 CGGCCG Eco47III 271 AGCGCT AGC^GCT 509 AGCGCT Eco52I 482 CGGCCG C^GGCCG 771 CGGCCG Eco81I 1603 CCTGAGG CC^T[ACGT]AGG 2398 CCTCAGG EcoT14I 959 CCAAGG C^C[AT][AT]GG 1637 CCATGG Hin1I 567 GACGCC G[GA]^CG[CT]C 2325 GGCGCC MslI 1488 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2161 CACAGGTATG SfiI 483 GGCCGCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 948 GGCCATCCAGGCC StuI 524 AGGCCT AGG^CCT 1211 AGGCCT StyI 959 CCAAGG C^C[AT][AT]GG 1637 CCATGG XcmI 1073 CCAGGCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1733 CCATCATCCAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaI AvaII BalI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI Tru9I TseI Tsp45I TspRI Van91I XhoII XmaCI XmaI