(Circular) (Six-base) MAPSORT of: pFN21AB7066.seq Check: 4027 from: 1 to: 7150 pFN21AB7066 7150 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2379 3672 2379 Size: 1293 5857 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4960 278 Size: 53 83 186 936 3424 2468 Fragments arranged by size: 3424 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4493 1937 Size: 2556 4594 Acc65I G'GTAC_C Cuts at: 4471 4471 Size: 7150 AclI AA'CG_TT Cuts at: 1563 5277 5650 1563 Size: 3714 373 3063 Fragments arranged by size: 3714 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3309 3848 4094 4148 4256 5213 6597 6865 Size: 1677 539 246 54 108 957 1384 268 Cuts at: 6865 1632 Size: 1917 Fragments arranged by size: 1917 1677 1384 957 539 268 246 108 54 AfeI AGC'GCT Cuts at: 2278 2516 2278 Size: 238 6912 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6953 AflIII A'CryG_T Cuts at: 1203 3564 6049 7026 1203 Size: 2361 2485 977 1327 Fragments arranged by size: 2485 2361 1327 977 AgeI A'CCGG_T Cuts at: 3273 5981 3273 Size: 2708 4442 AhdI GACnn_n'nnGTC Cuts at: 5879 5879 Size: 7150 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7150 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2960 1184 Size: 1776 5374 AlwNI CAG_nnn'CTG Cuts at: 3448 3472 4893 6465 3448 Size: 24 1421 1572 4133 Fragments arranged by size: 4133 1572 1421 24 ApaI G_GGCC'C Cuts at: 2859 3013 2859 Size: 154 6996 ApaLI G'TGCA_C Cuts at: 5207 6363 5207 Size: 1156 5994 ApoI r'AATT_y Cuts at: 1493 4459 4685 4837 6946 1493 Size: 2966 226 152 2109 1697 Fragments arranged by size: 2966 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5704 160 Size: 872 4672 1606 Fragments arranged by size: 4672 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7150 AvaI C'yCGr_G Cuts at: 1870 1943 2143 2710 2910 3097 3874 4475 1870 Size: 73 200 567 200 187 777 601 4545 Fragments arranged by size: 4545 777 601 567 200 200 187 73 BamHI G'GATC_C Cuts at: 4108 4480 4847 4108 Size: 372 367 6411 Fragments arranged by size: 6411 372 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4330 4471 5926 618 Size: 325 217 627 2543 141 1455 1842 Fragments arranged by size: 2543 1842 1455 627 325 217 141 BanII G_rGCy'C Cuts at: 1391 2695 2703 2859 3013 3104 3919 4469 1391 Size: 1304 8 156 154 91 815 550 4072 Fragments arranged by size: 4072 1304 815 550 156 154 91 8 BbeI G_GCGC'C Cuts at: 4334 4334 Size: 7150 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3783 3867 4368 928 Size: 971 1884 84 501 3710 Fragments arranged by size: 3710 1884 971 501 84 BbvCI CC'TCA_GC Cuts at: 2684 3019 2684 Size: 335 6815 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2181 2215 2466 2500 5341 5375 1903 Size: 34 244 34 251 34 2841 34 3678 Fragments arranged by size: 3678 2841 251 244 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3957 5042 6252 3957 Size: 1085 1210 4855 Fragments arranged by size: 4855 1210 1085 BclI T'GATC_A Cuts at: 1548 1548 Size: 7150 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2172 2495 2960 Size: 107 122 71 631 605 500 323 465 Cuts at: 2960 3692 4155 5761 136 Size: 732 463 1606 1525 Fragments arranged by size: 1606 1525 732 631 605 500 465 463 323 122 107 71 BglII A'GATC_T Cuts at: 7145 7145 Size: 7150 BlpI GC'TnA_GC Cuts at: 2589 4567 2589 Size: 1978 5172 Bme1580I G_kGCm'C Cuts at: 1790 2132 2859 3013 4210 5211 6367 1790 Size: 342 727 154 1197 1001 1156 2573 Fragments arranged by size: 2573 1197 1156 1001 727 342 154 BmgBI CAC'GTC Cuts at: 1318 3534 1318 Size: 2216 4934 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3538 4881 5839 Size: 416 53 83 399 24 2121 1343 958 Cuts at: 5839 442 Size: 1753 Fragments arranged by size: 2121 1753 1343 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2546 3209 3823 4370 5810 1239 Size: 1307 663 614 547 1440 2579 Fragments arranged by size: 2579 1440 1307 663 614 547 Bpu10I CC'TnA_GC Cuts at: 2684 3019 3398 4386 2684 Size: 335 379 988 5448 Fragments arranged by size: 5448 988 379 335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4049 5275 6140 6438 4049 Size: 1226 865 298 4761 Fragments arranged by size: 4761 1226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5813 882 Size: 617 4314 2219 Fragments arranged by size: 4314 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3085 4941 493 Size: 711 1881 1856 2702 Fragments arranged by size: 2702 1881 1856 711 BsaBI GATnn'nnATC Cuts at: 7144 7144 Size: 7150 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2573 4331 4957 5339 Size: 53 83 186 936 1040 1758 626 382 Cuts at: 5339 5974 275 Size: 635 1451 Fragments arranged by size: 1758 1451 1040 936 635 626 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3273 3988 5583 5981 6255 6402 6813 3273 Size: 715 1595 398 274 147 411 3610 Fragments arranged by size: 3610 1595 715 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2773 2803 2873 2903 3700 Size: 30 165 30 1370 30 70 30 797 Cuts at: 3700 3730 1178 Size: 30 4598 Fragments arranged by size: 4598 1370 797 165 70 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2781 4333 1186 Size: 913 682 1552 4003 Fragments arranged by size: 4003 1552 913 682 BseYI C'CCAG_C Cuts at: 2105 2273 2511 2520 2730 3441 3637 4292 6353 Size: 168 238 9 210 711 196 655 2061 Cuts at: 6353 6849 2105 Size: 496 2406 Fragments arranged by size: 2406 2061 711 655 496 238 210 196 168 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2378 2496 3668 3695 3828 1163 Size: 573 642 118 1172 27 133 4485 Fragments arranged by size: 4485 1172 642 573 133 118 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2490 2779 3486 4600 5361 6389 Size: 275 186 489 289 707 1114 761 1028 Cuts at: 6389 1540 Size: 2301 Fragments arranged by size: 2301 1114 1028 761 707 489 289 275 186 BsiHKAI G_wGCw'C Cuts at: 3912 5211 5296 6367 3912 Size: 1299 85 1071 4695 Fragments arranged by size: 4695 1299 1071 85 BsiWI C'GTAC_G Cuts at: 2319 3069 2319 Size: 750 6400 BsmI GAATG_Cn' Cuts at: 2039 4661 4754 2039 Size: 2622 93 4435 Fragments arranged by size: 4435 2622 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 2695 2703 2859 3013 3104 3912 Size: 399 342 563 8 156 154 91 808 Cuts at: 3912 3919 4210 4469 5211 5296 6367 1391 Size: 7 291 259 742 85 1071 2174 Fragments arranged by size: 2174 1071 808 742 563 399 342 291 259 156 154 91 85 8 7 BspEI T'CCGG_A Cuts at: 3988 6813 3988 Size: 2825 4325 BspHI T'CATG_A Cuts at: 3726 5039 6769 3726 Size: 1313 1730 4107 Fragments arranged by size: 4107 1730 1313 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2379 3672 4505 844 Size: 1535 1293 833 3489 Fragments arranged by size: 3489 1535 1293 833 BsrBI CCG'CTC Cuts at: 1394 1823 2023 3402 5037 1394 Size: 429 200 1379 1635 3507 Fragments arranged by size: 3507 1635 1379 429 200 BsrDI GCAATG_nn' Cuts at: 65 1248 5645 5819 65 Size: 1183 4397 174 1396 Fragments arranged by size: 4397 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2073 2188 2774 2940 2997 3273 3428 Size: 110 263 115 586 166 57 276 155 Cuts at: 3428 4431 5794 5981 1700 Size: 1003 1363 187 2869 Fragments arranged by size: 2869 1363 1003 586 276 263 187 166 155 115 110 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7150 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7150 BssSI C'ACGA_G Cuts at: 5210 6222 5210 Size: 1012 6138 BstAPI GCAn_nnn'nTGC Cuts at: 794 2034 794 Size: 1240 5910 BstBI TT'CG_AA Cuts at: 5954 5954 Size: 7150 BstEII G'GTnAC_C Cuts at: 1184 3619 1184 Size: 2435 4715 BstXI CCAn_nnnn'nTGG Cuts at: 2257 2393 2411 2479 2545 3085 2257 Size: 136 18 68 66 540 6322 Fragments arranged by size: 6322 540 136 68 66 18 BstYI r'GATC_y Cuts at: 1972 2058 3657 4108 4480 4847 5232 5249 6701 Size: 86 1599 451 372 367 385 17 1452 Cuts at: 6701 6787 6799 7145 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1599 1452 451 385 372 367 346 86 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3609 4404 3609 Size: 795 6355 BtgI C'CryG_G Cuts at: 513 1065 2167 2295 3354 3642 3681 4104 4302 Size: 552 1102 128 1059 288 39 423 198 Cuts at: 4302 513 Size: 3361 Fragments arranged by size: 3361 1102 1059 552 423 288 198 128 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6411 Fragments arranged by size: 6411 683 56 BtsI GCAGTG_nn' Cuts at: 2484 3561 4666 5460 5480 2484 Size: 1077 1105 794 20 4154 Fragments arranged by size: 4154 1105 1077 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7150 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2137 2172 529 Size: 35 1573 35 5507 Fragments arranged by size: 5507 1573 35 35 DraI TTT'AAA Cuts at: 4454 4454 Size: 7150 DraIII CAC_nnn'GTG Cuts at: 2122 3088 3320 3451 2122 Size: 966 232 131 5821 Fragments arranged by size: 5821 966 232 131 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2347 6157 809 Size: 507 1031 3810 1802 Fragments arranged by size: 3810 1802 1031 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2158 2298 2487 Size: 54 1425 50 275 18 328 140 189 Cuts at: 2487 2547 2776 2796 3268 3403 3522 3750 3882 Size: 60 229 20 472 135 119 228 132 Cuts at: 3882 4597 5486 7115 8 Size: 715 889 1629 43 Fragments arranged by size: 1629 1425 889 715 472 328 275 229 228 189 140 135 132 119 60 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2487 2776 4597 1537 Size: 275 675 289 1821 4090 Fragments arranged by size: 4090 1821 675 289 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2987 4310 4386 5080 1353 Size: 1634 1323 76 694 3423 Fragments arranged by size: 3423 1634 1323 694 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5737 6111 6257 387 Size: 1064 138 4148 374 146 1280 Fragments arranged by size: 4148 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2546 3209 3309 3823 3848 4094 4148 Size: 393 914 663 100 514 25 246 54 Cuts at: 4148 4256 4370 5213 5810 6597 6865 1239 Size: 108 114 843 597 787 268 1524 Fragments arranged by size: 1524 914 843 787 663 597 514 393 268 246 114 108 100 54 25 EcoNI CCTnn'n_nnAGG Cuts at: 3038 3038 Size: 7150 EcoO109I rG'GnC_Cy Cuts at: 2092 2196 2340 2397 2715 2855 2856 2971 3009 Size: 104 144 57 318 140 1 115 38 Cuts at: 3009 3010 3613 2092 Size: 1 603 5629 Fragments arranged by size: 5629 603 318 144 140 115 104 57 38 1 1 EcoRI G'AATT_C Cuts at: 4459 4837 4459 Size: 378 6772 FspI TGC'GCA Cuts at: 5656 5656 Size: 7150 HaeII r_GCGC'y Cuts at: 1678 2280 2518 4271 4334 6297 1678 Size: 602 238 1753 63 1963 2531 Fragments arranged by size: 2531 1963 1753 602 238 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3906 3938 5791 5823 5865 Size: 32 135 32 2334 32 1853 32 42 Cuts at: 5865 5897 1373 Size: 32 2626 Fragments arranged by size: 2626 2334 1853 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4494 4740 669 Size: 988 281 2556 246 3079 Fragments arranged by size: 3079 2556 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7150 HpaI GTT'AAC Cuts at: 4740 4740 Size: 7150 KasI G'GCGC_C Cuts at: 4330 4330 Size: 7150 KpnI G_GTAC'C Cuts at: 4475 4475 Size: 7150 MfeI C'AATT_G Cuts at: 4749 4749 Size: 7150 MluI A'CGCG_T Cuts at: 7026 7026 Size: 7150 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 6264 6448 1305 Size: 732 4227 184 2007 Fragments arranged by size: 4227 2007 732 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2300 2549 3270 3752 10 Size: 54 1425 343 468 249 721 482 3408 Fragments arranged by size: 3408 1425 721 482 468 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 3497 4170 5108 5467 5626 518 Size: 648 2331 673 938 359 159 2042 Fragments arranged by size: 2331 2042 938 673 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2443 2524 3182 4304 4566 5243 6391 6636 Size: 837 81 658 1122 262 677 1148 245 Cuts at: 6636 1606 Size: 2120 Fragments arranged by size: 2120 1148 1122 837 677 658 262 245 81 NaeI GCC'GGC Cuts at: 2190 2942 2999 3430 2190 Size: 752 57 431 5910 Fragments arranged by size: 5910 752 431 57 NarI GG'CG_CC Cuts at: 4331 4331 Size: 7150 NcoI C'CATG_G Cuts at: 513 1065 3642 513 Size: 552 2577 4021 Fragments arranged by size: 4021 2577 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7150 NgoMIV G'CCGG_C Cuts at: 2188 2940 2997 3428 2188 Size: 752 57 431 5910 Fragments arranged by size: 5910 752 431 57 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7150 NotI GC'GGCC_GC Cuts at: 4597 4597 Size: 7150 NspI r_CATG'y Cuts at: 1132 1229 4508 6053 1132 Size: 97 3279 1545 2229 Fragments arranged by size: 3279 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2851 1348 Size: 1503 5647 PciI A'CATG_T Cuts at: 6049 6049 Size: 7150 PflMI CCAn_nnn'nTGG Cuts at: 1108 2122 3448 4105 1108 Size: 1014 1326 657 4153 Fragments arranged by size: 4153 1326 1014 657 PmeI GTTT'AAAC Cuts at: 4454 4454 Size: 7150 PmlI CAC'GTG Cuts at: 3085 3085 Size: 7150 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5219 5251 6732 6764 5219 Size: 32 1481 32 5605 Fragments arranged by size: 5605 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2196 2340 2397 2715 2971 2196 Size: 144 57 318 256 6375 Fragments arranged by size: 6375 318 256 144 57 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7150 PsiI TTA'TAA Cuts at: 4720 4720 Size: 7150 PspOMI G'GGCC_C Cuts at: 2855 3009 2855 Size: 154 6996 PstI C_TGCA'G Cuts at: 830 2949 3556 4267 4502 830 Size: 2119 607 711 235 3478 Fragments arranged by size: 3478 2119 711 607 235 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7150 PvuII CAG'CTG Cuts at: 2443 2524 3182 2443 Size: 81 658 6411 Fragments arranged by size: 6411 658 81 SacII CC_GC'GG Cuts at: 4305 4305 Size: 7150 SalI G'TCGA_C Cuts at: 1936 4492 1936 Size: 2556 4594 SanDI GG'GwC_CC Cuts at: 2340 2715 2340 Size: 375 6775 SapI GCTCTTCn'nnn_ Cuts at: 2987 2987 Size: 7150 SbfI CC_TGCA'GG Cuts at: 4502 4502 Size: 7150 ScaI AGT'ACT Cuts at: 5398 7110 5398 Size: 1712 5438 SexAI A'CCwGG_T Cuts at: 3447 3447 Size: 7150 SfcI C'TryA_G Cuts at: 826 1045 2945 3112 3552 4263 4498 5633 6314 Size: 219 1900 167 440 711 235 1135 681 Cuts at: 6314 6505 826 Size: 191 1471 Fragments arranged by size: 1900 1471 1135 711 681 440 235 219 191 167 SfiI GGCCn_nnn'nGGCC Cuts at: 2495 2960 2495 Size: 465 6685 SfoI GGC'GCC Cuts at: 4332 4332 Size: 7150 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7150 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7150 SmaI CCC'GGG Cuts at: 1872 2145 2712 3876 4477 1872 Size: 273 567 1164 601 4545 Fragments arranged by size: 4545 1164 601 567 273 SmlI C'TyrA_G Cuts at: 820 1017 1943 4064 5254 6155 6417 820 Size: 197 926 2121 1190 901 262 1553 Fragments arranged by size: 2121 1553 1190 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4941 493 Size: 4448 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7150 SphI G_CATG'C Cuts at: 1132 4508 1132 Size: 3376 3774 SrfI GCCC'GGGC Cuts at: 3876 3876 Size: 7150 SspI AAT'ATT Cuts at: 5 52 5074 5 Size: 47 5022 2081 Fragments arranged by size: 5022 2081 47 StuI AGG'CCT Cuts at: 1517 2531 3218 1517 Size: 1014 687 5449 Fragments arranged by size: 5449 1014 687 StyI C'CwwG_G Cuts at: 513 1065 2964 3642 4589 513 Size: 552 1899 678 947 3074 Fragments arranged by size: 3074 1899 947 678 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3638 5177 5194 5347 5532 5977 325 Size: 1255 2058 1539 17 153 185 445 1498 Fragments arranged by size: 2058 1539 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5396 7108 96 Size: 274 80 33 51 1443 3419 1712 138 Fragments arranged by size: 3419 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 2773 2805 3568 3600 7110 7142 2430 Size: 32 311 32 763 32 3510 32 2438 Fragments arranged by size: 3510 2438 763 311 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7150 XbaI T'CTAG_A Cuts at: 4486 4486 Size: 7150 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3085 3745 3085 Size: 660 6490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7150 XmaI C'CCGG_G Cuts at: 1870 2143 2710 3874 4475 1870 Size: 273 567 1164 601 4545 Fragments arranged by size: 4545 1164 601 567 273 XmnI GAAnn'nnTTC Cuts at: 5279 5279 Size: 7150 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4958 276 Size: 53 83 186 936 3424 2468 Fragments arranged by size: 3424 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SwaI