Restriction map for
pFN21AB7066
(Linear) (Six-base) MAPSORT of: KIBB7066.seq Check: 8583 from: 1 to: 2445
KIBB7066.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 374 1667 2445
Size: 374 1293 778
Fragments arranged by size:
1293 778 374
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1304 1843 2089 2143 2251 2445
Size: 1304 539 246 54 108 194
Fragments arranged by size:
1304 539 246 194 108 54
AfeI AGC'GCT
Cuts at: 0 273 511 2445
Size: 273 238 1934
Fragments arranged by size:
1934 273 238
AflIII A'CryG_T
Cuts at: 0 1559 2445
Size: 1559 886
AgeI A'CCGG_T
Cuts at: 0 1268 2445
Size: 1268 1177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 955 2445
Size: 955 1490
AlwNI CAG_nnn'CTG
Cuts at: 0 1443 1467 2445
Size: 1443 24 978
Fragments arranged by size:
1443 978 24
ApaI G_GGCC'C
Cuts at: 0 854 1008 2445
Size: 854 154 1437
Fragments arranged by size:
1437 854 154
AvaI C'yCGr_G
Cuts at: 0 138 705 905 1092 1869 2445
Size: 138 567 200 187 777 576
Fragments arranged by size:
777 576 567 200 187 138
BamHI G'GATC_C
Cuts at: 0 2103 2445
Size: 2103 342
BanI G'GyrC_C
Cuts at: 0 2325 2445
Size: 2325 120
BanII G_rGCy'C
Cuts at: 0 690 698 854 1008 1099 1914 2445
Size: 690 8 156 154 91 815 531
Fragments arranged by size:
815 690 531 156 154 91 8
BbeI G_GCGC'C
Cuts at: 0 2329 2445
Size: 2329 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 1778 1862 2363 2445
Size: 1778 84 501 82
Fragments arranged by size:
1778 501 84 82
BbvCI CC'TCA_GC
Cuts at: 0 679 1014 2445
Size: 679 335 1431
Fragments arranged by size:
1431 679 335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 176 210 461 495 2445
Size: 176 34 251 34 1950
Fragments arranged by size:
1950 251 176 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1952 2445
Size: 1952 493
BglI GCCn_nnn'nGGC
Cuts at: 0 167 490 955 1687 2150 2445
Size: 167 323 465 732 463 295
Fragments arranged by size:
732 465 463 323 295 167
BlpI GC'TnA_GC
Cuts at: 0 584 2445
Size: 584 1861
Bme1580I G_kGCm'C
Cuts at: 0 127 854 1008 2205 2445
Size: 127 727 154 1197 240
Fragments arranged by size:
1197 727 240 154 127
BmgBI CAC'GTC
Cuts at: 0 1529 2445
Size: 1529 916
BmrI ACTGGGnnnn_n'
Cuts at: 0 1533 2445
Size: 1533 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 1204 1818 2365 2445
Size: 541 663 614 547 80
Fragments arranged by size:
663 614 547 541 80
Bpu10I CC'TnA_GC
Cuts at: 0 679 1014 1393 2381 2445
Size: 679 335 379 988 64
Fragments arranged by size:
988 679 379 335 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2044 2445
Size: 2044 401
BsaAI yAC'GTr
Cuts at: 0 1080 2445
Size: 1080 1365
BsaHI Gr'CG_yC
Cuts at: 0 568 2326 2445
Size: 568 1758 119
Fragments arranged by size:
1758 568 119
BsaWI w'CCGG_w
Cuts at: 0 1268 1983 2445
Size: 1268 715 462
Fragments arranged by size:
1268 715 462
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 768 798 868 898 1695 1725 2445
Size: 768 30 70 30 797 30 720
Fragments arranged by size:
797 768 720 70 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 776 2328 2445
Size: 94 682 1552 117
Fragments arranged by size:
1552 682 117 94
BseYI C'CCAG_C
Cuts at: 0 100 268 506 515 725 1436 1632 2287
Size: 100 168 238 9 210 711 196 655
Cuts at: 2287 2445
Size: 158
Fragments arranged by size:
711 655 238 210 196 168 158 100
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 491 1663 1690 1823 2445
Size: 373 118 1172 27 133 622
Fragments arranged by size:
1172 622 373 133 118 27
BsiEI CG_ry'CG
Cuts at: 0 485 774 1481 2445
Size: 485 289 707 964
Fragments arranged by size:
964 707 485 289
BsiHKAI G_wGCw'C
Cuts at: 0 1907 2445
Size: 1907 538
BsiWI C'GTAC_G
Cuts at: 0 314 1064 2445
Size: 314 750 1381
Fragments arranged by size:
1381 750 314
BsmI GAATG_Cn'
Cuts at: 0 34 2445
Size: 34 2411
Bsp1286I G_dGCh'C
Cuts at: 0 127 690 698 854 1008 1099 1907 1914
Size: 127 563 8 156 154 91 808 7
Cuts at: 1914 2205 2445
Size: 291 240
Fragments arranged by size:
808 563 291 240 156 154 127 91
8 7
BspEI T'CCGG_A
Cuts at: 0 1983 2445
Size: 1983 462
BspHI T'CATG_A
Cuts at: 0 1721 2445
Size: 1721 724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 374 1667 2445
Size: 374 1293 778
Fragments arranged by size:
1293 778 374
BsrBI CCG'CTC
Cuts at: 0 18 1397 2445
Size: 18 1379 1048
Fragments arranged by size:
1379 1048 18
BsrFI r'CCGG_y
Cuts at: 0 68 183 769 935 992 1268 1423 2426
Size: 68 115 586 166 57 276 155 1003
Cuts at: 2426 2445
Size: 19
Fragments arranged by size:
1003 586 276 166 155 115 68 57
19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 29 2445
Size: 29 2416
BstEII G'GTnAC_C
Cuts at: 0 1614 2445
Size: 1614 831
BstXI CCAn_nnnn'nTGG
Cuts at: 0 252 388 406 474 540 1080 2445
Size: 252 136 18 68 66 540 1365
Fragments arranged by size:
1365 540 252 136 68 66 18
BstYI r'GATC_y
Cuts at: 0 53 1652 2103 2445
Size: 53 1599 451 342
Fragments arranged by size:
1599 451 342 53
Bsu36I CC'TnA_GG
Cuts at: 0 1604 2399 2445
Size: 1604 795 46
Fragments arranged by size:
1604 795 46
BtgI C'CryG_G
Cuts at: 0 162 290 1349 1637 1676 2099 2297 2445
Size: 162 128 1059 288 39 423 198 148
Fragments arranged by size:
1059 423 288 198 162 148 128 39
BtsI GCAGTG_nn'
Cuts at: 0 479 1556 2445
Size: 479 1077 889
Fragments arranged by size:
1077 889 479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 132 167 2445
Size: 132 35 2278
Fragments arranged by size:
2278 132 35
DraIII CAC_nnn'GTG
Cuts at: 0 117 1083 1315 1446 2445
Size: 117 966 232 131 999
Fragments arranged by size:
999 966 232 131 117
DrdI GACnn_nn'nnGTC
Cuts at: 0 342 2445
Size: 342 2103
EaeI y'GGCC_r
Cuts at: 0 153 293 482 542 771 791 1263 1398
Size: 153 140 189 60 229 20 472 135
Cuts at: 1398 1517 1745 1877 2445
Size: 119 228 132 568
Fragments arranged by size:
568 472 229 228 189 153 140 135
132 119 60 20
EagI C'GGCC_G
Cuts at: 0 482 771 2445
Size: 482 289 1674
Fragments arranged by size:
1674 482 289
EarI CTCTTCn'nnn_
Cuts at: 0 982 2305 2381 2445
Size: 982 1323 76 64
Fragments arranged by size:
1323 982 76 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 1204 1304 1818 1843 2089 2143 2251
Size: 541 663 100 514 25 246 54 108
Cuts at: 2251 2365 2445
Size: 114 80
Fragments arranged by size:
663 541 514 246 114 108 100 80
54 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1033 2445
Size: 1033 1412
EcoO109I rG'GnC_Cy
Cuts at: 0 87 191 335 392 710 850 851 966
Size: 87 104 144 57 318 140 1 115
Cuts at: 966 1004 1005 1608 2445
Size: 38 1 603 837
Fragments arranged by size:
837 603 318 144 140 115 104 87
57 38 1 1
HaeII r_GCGC'y
Cuts at: 0 275 513 2266 2329 2445
Size: 275 238 1753 63 116
Fragments arranged by size:
1753 275 238 116 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1901 1933 2445
Size: 1901 32 512
Fragments arranged by size:
1901 512 32
KasI G'GCGC_C
Cuts at: 0 2325 2445
Size: 2325 120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 2445
Size: 32 2413
MscI TGG'CCA
Cuts at: 0 295 544 1265 1747 2445
Size: 295 249 721 482 698
Fragments arranged by size:
721 698 482 295 249
MslI CAynn'nnrTG
Cuts at: 0 1492 2165 2445
Size: 1492 673 280
Fragments arranged by size:
1492 673 280
MspA1I CmG'CkG
Cuts at: 0 438 519 1177 2299 2445
Size: 438 81 658 1122 146
Fragments arranged by size:
1122 658 438 146 81
NaeI GCC'GGC
Cuts at: 0 185 937 994 1425 2445
Size: 185 752 57 431 1020
Fragments arranged by size:
1020 752 431 185 57
NarI GG'CG_CC
Cuts at: 0 2326 2445
Size: 2326 119
NcoI C'CATG_G
Cuts at: 0 1637 2445
Size: 1637 808
NgoMIV G'CCGG_C
Cuts at: 0 183 935 992 1423 2445
Size: 183 752 57 431 1022
Fragments arranged by size:
1022 752 431 183 57
PasI CC'CwG_GG
Cuts at: 0 846 2445
Size: 846 1599
PflMI CCAn_nnn'nTGG
Cuts at: 0 117 1443 2100 2445
Size: 117 1326 657 345
Fragments arranged by size:
1326 657 345 117
PmlI CAC'GTG
Cuts at: 0 1080 2445
Size: 1080 1365
PpuMI rG'GwC_Cy
Cuts at: 0 191 335 392 710 966 2445
Size: 191 144 57 318 256 1479
Fragments arranged by size:
1479 318 256 191 144 57
PspOMI G'GGCC_C
Cuts at: 0 850 1004 2445
Size: 850 154 1441
Fragments arranged by size:
1441 850 154
PstI C_TGCA'G
Cuts at: 0 944 1551 2262 2445
Size: 944 607 711 183
Fragments arranged by size:
944 711 607 183
PvuII CAG'CTG
Cuts at: 0 438 519 1177 2445
Size: 438 81 658 1268
Fragments arranged by size:
1268 658 438 81
SacII CC_GC'GG
Cuts at: 0 2300 2445
Size: 2300 145
SanDI GG'GwC_CC
Cuts at: 0 335 710 2445
Size: 335 375 1735
Fragments arranged by size:
1735 375 335
SapI GCTCTTCn'nnn_
Cuts at: 0 982 2445
Size: 982 1463
SexAI A'CCwGG_T
Cuts at: 0 1442 2445
Size: 1442 1003
SfcI C'TryA_G
Cuts at: 0 940 1107 1547 2258 2445
Size: 940 167 440 711 187
Fragments arranged by size:
940 711 440 187 167
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 490 955 2445
Size: 490 465 1490
Fragments arranged by size:
1490 490 465
SfoI GGC'GCC
Cuts at: 0 2327 2445
Size: 2327 118
SmaI CCC'GGG
Cuts at: 0 140 707 1871 2445
Size: 140 567 1164 574
Fragments arranged by size:
1164 574 567 140
SmlI C'TyrA_G
Cuts at: 0 2059 2445
Size: 2059 386
SrfI GCCC'GGGC
Cuts at: 0 1871 2445
Size: 1871 574
StuI AGG'CCT
Cuts at: 0 526 1213 2445
Size: 526 687 1232
Fragments arranged by size:
1232 687 526
StyI C'CwwG_G
Cuts at: 0 959 1637 2445
Size: 959 678 808
Fragments arranged by size:
959 808 678
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1633 2445
Size: 1633 812
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 768 800 1563 1595 2445
Size: 425 32 311 32 763 32 850
Fragments arranged by size:
850 763 425 311 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1080 1740 2445
Size: 1080 660 705
Fragments arranged by size:
1080 705 660
XmaI C'CCGG_G
Cuts at: 0 138 705 1869 2445
Size: 138 567 1164 576
Fragments arranged by size:
1164 576 567 138
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI DraIII DrdI EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PmlI
PpuMI PspOMI PstI PvuII SacII SanDI SapI SexAI
SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BclI
BfrBI BglII BmtI BplI BsaI BsaBI BsmBI BsrDI
BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI