################################## # NO CUT ENZYMES ( 200 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvaI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AlwI 206 GATCC GGATC(4/5) BanI 147 GGTGCC G^G[CT][GA]CC BbsI 88 GTCTTC GAAGAC(2/6) BclI 41 TGATCA T^GATCA BpuAI 88 GTCTTC GAAGAC(2/6) BsiYI 120 CCGCATTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 120 CCGCATTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 187 GGGAC GGGAC(10/14) BsmI 122 GCATTC GAATGC(1/-1) BssHII 72 GCGCGC G^CGCGC BstF5I 29 GGATG GGATG(2/0) BstYI 205 AGATCC [GA]^GATC[CT] Cac8I 72 GCGCGC GC[ACGT]^[ACGT]GC Cfr13I 3 GGGCC G^G[ACGT]CC EarI 193 GAAGAG CTCTTC(1/4) FbaI 41 TGATCA T^GATCA FokI 29 GGATG GGATG(9/13) HaeIII 4 GGCC GG^CC HgaI 24 GACGC GACGC(5/10) HphI 58 TCACC GGTGA(8/7) Ksp632I 193 GAAGAG CTCTTC(1/4) MaeII 86 ACGT A^CGT MaeIII 57 GTCAC ^GT[ACGT]AC MflI 205 AGATCC [GA]^GATC[CT] MnlI 213 GAGG CCTC(7/6) MspA1I 224 CAGCTG C[AC]G^C[GT]G MwoI 27 GCGGATGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 147 GGTGCC GG[ACGT]^[ACGT]CC NspI 36 GCATGT [GA]CATG^[CT] PleI 130 GAGTC GAGTC(4/5) PvuII 224 CAGCTG CAG^CTG Sau96I 3 GGGCC G^G[ACGT]CC SfaNI 30 GATGC GCATC(5/9) SfcI 48 CTACAG C^T[GA][CT]AG Tsp45I 57 GTCAC ^GT[GC]AC XhoII 205 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BbvI 69 GCAGC GCAGC(8/12) 226 GCTGC DpnII 42 GATC ^GATC 206 GATC MboI 42 GATC ^GATC 206 GATC NdeII 42 GATC ^GATC 206 GATC NlaIII 37 CATG CATG^ 182 CATG Sau3AI 42 GATC ^GATC 206 GATC TaqI 65 TCGA T^CGA 200 TCGA TseI 69 GCAGC G^C[AT]GC 226 GCTGC TthHB8I 65 TCGA T^CGA 200 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AccII AciI AluI BstUI CfoI Fnu4HI HhaI HinP1I HinfI ItaI MboII MvnI TfiI