(Circular) (Six-base) MAPSORT of: pFN21AE6172.seq Check: 4355 from: 1 to: 4951 pFN21AE6172 4951 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2761 278 Size: 53 83 186 936 1225 2468 Fragments arranged by size: 2468 1225 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2294 1937 Size: 357 4594 Acc65I G'GTAC_C Cuts at: 2272 2272 Size: 4951 AclI AA'CG_TT Cuts at: 1563 3078 3451 1563 Size: 1515 373 3063 Fragments arranged by size: 3063 1515 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2217 3014 4398 4666 1632 Size: 585 797 1384 268 1917 Fragments arranged by size: 1917 1384 797 585 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4754 AflIII A'CryG_T Cuts at: 1203 3850 4827 1203 Size: 2647 977 1327 Fragments arranged by size: 2647 1327 977 AgeI A'CCGG_T Cuts at: 3782 3782 Size: 4951 AhdI GACnn_n'nnGTC Cuts at: 3680 3680 Size: 4951 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4951 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4951 AlwNI CAG_nnn'CTG Cuts at: 2694 4266 2694 Size: 1572 3379 ApaLI G'TGCA_C Cuts at: 3008 4164 3008 Size: 1156 3795 ApoI r'AATT_y Cuts at: 1493 2260 2486 2638 4747 1493 Size: 767 226 152 2109 1697 Fragments arranged by size: 2109 1697 767 226 152 AseI AT'TA_AT Cuts at: 160 1032 3505 160 Size: 872 2473 1606 Fragments arranged by size: 2473 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4951 AvaI C'yCGr_G Cuts at: 1870 1943 2276 1870 Size: 73 333 4545 Fragments arranged by size: 4545 333 73 BamHI G'GATC_C Cuts at: 2281 2648 2281 Size: 367 4584 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2152 2272 3727 618 Size: 325 217 627 365 120 1455 1842 Fragments arranged by size: 1842 1455 627 365 325 217 120 BanII G_rGCy'C Cuts at: 1391 2270 1391 Size: 879 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2086 928 Size: 971 187 3793 Fragments arranged by size: 3793 971 187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3142 3176 1903 Size: 34 1205 34 3678 Fragments arranged by size: 3678 1205 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2843 4053 2843 Size: 1210 3741 BclI T'GATC_A Cuts at: 1548 2046 1548 Size: 498 4453 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3562 136 Size: 107 122 71 631 605 1890 1525 Fragments arranged by size: 1890 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4946 4946 Size: 4951 BlpI GC'TnA_GC Cuts at: 2368 2368 Size: 4951 Bme1580I G_kGCm'C Cuts at: 1790 3012 4168 1790 Size: 1222 1156 2573 Fragments arranged by size: 2573 1222 1156 BmgBI CAC'GTC Cuts at: 1318 2092 1318 Size: 774 4177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2682 3640 442 Size: 416 53 83 399 24 1265 958 1753 Fragments arranged by size: 1753 1265 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4951 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3611 1239 Size: 2372 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3076 3941 4239 3076 Size: 865 298 3788 Fragments arranged by size: 3788 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3614 882 Size: 617 2115 2219 Fragments arranged by size: 2219 2115 617 BsaAI yAC'GTr Cuts at: 493 1204 2742 493 Size: 711 1538 2702 Fragments arranged by size: 2702 1538 711 BsaBI GATnn'nnATC Cuts at: 4945 4945 Size: 4951 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2758 3140 3775 275 Size: 53 83 186 936 1225 382 635 1451 Fragments arranged by size: 1451 1225 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3384 3782 4056 4203 4614 3384 Size: 398 274 147 411 3721 Fragments arranged by size: 3721 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4726 Fragments arranged by size: 4726 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4951 BseYI C'CCAG_C Cuts at: 4154 4650 4154 Size: 496 4455 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2401 3162 4190 1540 Size: 275 186 400 761 1028 2301 Fragments arranged by size: 2301 1028 761 400 275 186 BsiHKAI G_wGCw'C Cuts at: 3012 3097 4168 3012 Size: 85 1071 3795 Fragments arranged by size: 3795 1071 85 BsmI GAATG_Cn' Cuts at: 2127 2462 2555 2127 Size: 335 93 4523 Fragments arranged by size: 4523 335 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2270 3012 3097 4168 1391 Size: 399 480 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 480 399 85 BspEI T'CCGG_A Cuts at: 4614 4614 Size: 4951 BspHI T'CATG_A Cuts at: 2840 4570 2840 Size: 1730 3221 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2306 844 Size: 1462 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2838 1394 Size: 429 1015 3507 Fragments arranged by size: 3507 1015 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3446 3620 65 Size: 1183 2198 174 1396 Fragments arranged by size: 2198 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3595 3782 1700 Size: 110 1785 187 2869 Fragments arranged by size: 2869 1785 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4951 BssHII G'CGCG_C Cuts at: 1925 2077 1925 Size: 152 4799 BssSI C'ACGA_G Cuts at: 3011 4023 3011 Size: 1012 3939 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4951 BstBI TT'CG_AA Cuts at: 3755 3755 Size: 4951 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4951 BstYI r'GATC_y Cuts at: 1972 2210 2281 2648 3033 3050 4502 4588 4600 Size: 238 71 367 385 17 1452 86 12 Cuts at: 4600 4946 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 238 86 71 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3460 Fragments arranged by size: 3460 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4212 Fragments arranged by size: 4212 683 56 BtsI GCAGTG_nn' Cuts at: 2467 3261 3281 2467 Size: 794 20 4137 Fragments arranged by size: 4137 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4951 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4916 DraI TTT'AAA Cuts at: 2255 2255 Size: 4951 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3958 809 Size: 507 2642 1802 Fragments arranged by size: 2642 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2398 3287 4916 Size: 54 1425 50 275 18 568 889 1629 Cuts at: 4916 8 Size: 43 Fragments arranged by size: 1629 1425 889 568 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2398 1537 Size: 275 586 4090 Fragments arranged by size: 4090 586 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2193 2881 1353 Size: 840 688 3423 Fragments arranged by size: 3423 840 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3538 3912 4058 387 Size: 1064 138 1949 374 146 1280 Fragments arranged by size: 1949 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 3014 3611 4398 4666 1239 Size: 393 585 797 597 787 268 1524 Fragments arranged by size: 1524 797 787 597 585 393 268 EcoRI G'AATT_C Cuts at: 2260 2638 2260 Size: 378 4573 FspI TGC'GCA Cuts at: 3457 3457 Size: 4951 HaeII r_GCGC'y Cuts at: 1678 4098 1678 Size: 2420 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2209 2241 3592 3624 3666 Size: 32 135 32 637 32 1351 32 42 Cuts at: 3666 3698 1373 Size: 32 2626 Fragments arranged by size: 2626 1351 637 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2295 2541 669 Size: 988 281 357 246 3079 Fragments arranged by size: 3079 988 357 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4951 HpaI GTT'AAC Cuts at: 2541 2541 Size: 4951 KpnI G_GTAC'C Cuts at: 2276 2276 Size: 4951 MfeI C'AATT_G Cuts at: 2550 2550 Size: 4951 MluI A'CGCG_T Cuts at: 4827 4827 Size: 4951 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4065 4249 1305 Size: 2760 184 2007 Fragments arranged by size: 2760 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3129 Fragments arranged by size: 3129 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2909 3268 3427 518 Size: 648 1743 359 159 2042 Fragments arranged by size: 2042 1743 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2231 2367 3044 4192 4437 1606 Size: 625 136 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 625 245 136 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3460 Fragments arranged by size: 3460 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4951 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4951 NotI GC'GGCC_GC Cuts at: 2398 2398 Size: 4951 NspI r_CATG'y Cuts at: 1132 1229 2045 2309 3854 1132 Size: 97 816 264 1545 2229 Fragments arranged by size: 2229 1545 816 264 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4951 PciI A'CATG_T Cuts at: 3850 3850 Size: 4951 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4951 PmeI GTTT'AAAC Cuts at: 2255 2255 Size: 4951 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3020 3052 4533 4565 3020 Size: 32 1481 32 3406 Fragments arranged by size: 3406 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4951 PsiI TTA'TAA Cuts at: 2521 2521 Size: 4951 PstI C_TGCA'G Cuts at: 830 2303 830 Size: 1473 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4951 PvuII CAG'CTG Cuts at: 2231 2231 Size: 4951 SalI G'TCGA_C Cuts at: 1936 2293 1936 Size: 357 4594 SbfI CC_TGCA'GG Cuts at: 2303 2303 Size: 4951 ScaI AGT'ACT Cuts at: 3199 4911 3199 Size: 1712 3239 SfcI C'TryA_G Cuts at: 826 1045 2053 2299 3434 4115 4306 826 Size: 219 1008 246 1135 681 191 1471 Fragments arranged by size: 1471 1135 1008 681 246 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4951 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4951 SmaI CCC'GGG Cuts at: 1872 2278 1872 Size: 406 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3055 3956 4218 820 Size: 197 926 1112 901 262 1553 Fragments arranged by size: 1553 1112 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2742 493 Size: 2249 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4951 SphI G_CATG'C Cuts at: 1132 2309 1132 Size: 1177 3774 SspI AAT'ATT Cuts at: 5 52 2875 5 Size: 47 2823 2081 Fragments arranged by size: 2823 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4951 StyI C'CwwG_G Cuts at: 513 1065 2004 2390 513 Size: 552 939 386 3074 Fragments arranged by size: 3074 939 552 386 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2978 2995 3148 3333 3778 325 Size: 1255 1398 17 153 185 445 1498 Fragments arranged by size: 1498 1398 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3197 4909 96 Size: 274 80 33 51 1443 1220 1712 138 Fragments arranged by size: 1712 1443 1220 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4911 4943 4911 Size: 32 4919 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4951 XbaI T'CTAG_A Cuts at: 2287 2287 Size: 4951 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4951 XmaI C'CCGG_G Cuts at: 1870 2276 1870 Size: 406 4545 XmnI GAAnn'nnTTC Cuts at: 3080 3080 Size: 4951 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2759 276 Size: 53 83 186 936 1225 2468 Fragments arranged by size: 2468 1225 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI