################################## # NO CUT ENZYMES ( 220 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT22I FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AlwI 124 GGATC GGATC(4/5) Asp700I 146 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 91 GAGCCC G[GA]GC[CT]^C BbvCI 46 CCTCAGC CCTCAGC(-5/-2) BbvI 228 GCAGC GCAGC(8/12) BciVI 237 GGATAC GTATCC(6/5) BglI 51 GCCTAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 91 GAGCCC G[AGT]GC[ACT]^C BsaI 131 GAGACC GGTCTC(1/5) BsaJI 185 CCAAGG C^C[ACGT][ACGT]GG BsmAI 131 GAGAC GTCTC(1/5) BsmI 206 GAATGC GAATGC(1/-1) Bsp1286I 91 GAGCCC G[AGT]GC[ACT]^C BssSI 232 CTCGTG CACGAG(-5/-1) Cac8I 210 GCCTGC GC[ACGT]^[ACGT]GC DdeI 47 CTCAG C^T[ACGT]AG DpnII 125 GATC ^GATC EcoT14I 185 CCAAGG C^C[AT][AT]GG FauI 247 GCGGG CCCGC(4/6) Fnu4HI 228 GCAGC GC^[ACGT]GC ItaI 228 GCAGC GC^[ACGT]GC MboI 125 GATC ^GATC MwoI 51 GCCTAAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 125 GATC ^GATC NlaIII 118 CATG CATG^ Sau3AI 125 GATC ^GATC SfaNI 225 GATGC GCATC(5/9) StyI 185 CCAAGG C^C[AT][AT]GG TseI 228 GCAGC G^C[AT]GC TspRI 141 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 146 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### AciI 66 GCGG CCGC(-3/-1) 247 GCGG AcsI 7 GAATTC [GA]^AATT[CT] 150 GAATTC ApoI 7 GAATTC [GA]^AATT[CT] 150 GAATTC EcoRI 7 GAATTC G^AATTC 150 GAATTC Tsp509I 8 AATT ^AATT 151 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### AluI MnlI