################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI Eco47III EcoRI EcoRV FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeII MfeI MluI MroI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AflII 973 CTTAAG C^TTAAG AhdI 274 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 440 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 12 CCTGAGG CC^T[ACGT]AGG ApaI 464 GGGCCC GGGCC^C AspEI 274 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 215 GAGCAC G[AT]GC[AT]^C BbvCI 769 GCTGAGG CCTCAGC(-5/-2) BclI 837 TGATCA T^GATCA BfrI 973 CTTAAG C^TTAAG BglI 1279 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 835 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 28 CGGCCG CG[GA][CT]^CG BsiHKAI 215 GAGCAC G[AT]GC[AT]^C BsmFI 74 GGGAC GGGAC(10/14) BsmI 388 GAATGC GAATGC(1/-1) BspMI 310 GCAGGT ACCTGC(4/8) BssHII 60 GCGCGC G^CGCGC BstEII 313 GGTTACC G^GT[ACGT]ACC BstPI 313 GGTTACC G^GT[ACGT]ACC BstYI 774 GGATCT [GA]^GATC[CT] Bsu36I 12 CCTGAGG CC^T[ACGT]AGG DraI 1512 TTTAAA TTT^AAA DraII 726 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 674 CCATGG C^C[GA][CT]GG EagI 28 CGGCCG C^GGCCG Eam1105I 274 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 28 CGGCCG C^GGCCG Eco52I 28 CGGCCG C^GGCCG Eco81I 12 CCTGAGG CC^T[ACGT]AGG EcoNI 120 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 726 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 313 GGTTACC G^GT[ACGT]ACC EcoT22I 390 ATGCAT ATGCA^T FbaI 837 TGATCA T^GATCA MamI 835 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 774 GGATCT [GA]^GATC[CT] MspA1I 1280 CCGCTG C[AC]G^C[GT]G NcoI 674 CCATGG C^CATGG NsiI 390 ATGCAT ATGCA^T PflMI 343 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 76 GACTC GAGTC(4/5) PpuMI 726 AGGACCC [GA]G^G[AT]CC[CT] SapI 922 GAAGAGC GCTCTTC(1/4) SfiI 1278 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1472 CCCGGG CCC^GGG SmlI 973 CTTAAG C^T[CT][GA]AG SpeI 153 ACTAGT A^CTAGT Van91I 343 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1610 CCAGACTGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 774 GGATCT [GA]^GATC[CT] XmaCI 1472 CCCGGG C^CCGGG XmaI 1472 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### Acc65I 709 GGTACC G^GTACC 1091 GGTACC Asp718I 709 GGTACC G^GTACC 1091 GGTACC AvaI 212 CCCGAG C^[CT]CG[GA]G 1472 CCCGGG AvaII 727 GGACC G^G[AT]CC 1461 GGACC BalI 1393 TGGCCA TGG^CCA 1445 TGGCCA BbsI 1662 GAAGAC GAAGAC(2/6) 603 GTCTTC BpmI 1491 CTCCAG CTGGAG(16/14) 809 CTGGAG BpuAI 1662 GAAGAC GAAGAC(2/6) 603 GTCTTC BsgI 645 CTGCAC GTGCAG(16/14) 1637 GTGCAG BsoBI 212 CCCGAG C^[CT]CG[GA]G 1472 CCCGGG BsrDI 796 CATTGC GCAATG(2/0) 1002 CATTGC FauI 38 CCCGC CCCGC(4/6) 49 CCCGC HphI 202 GGTGA GGTGA(8/7) 905 TCACC KpnI 709 GGTACC GGTAC^C 1091 GGTACC MluNI 1393 TGGCCA TGG^CCA 1445 TGGCCA MscI 1393 TGGCCA TGG^CCA 1445 TGGCCA SfaNI 409 GATGC GCATC(5/9) 500 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BanI BanII BbvI BcnI BfaI BmyI BsaI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I