(Circular) (Six-base) MAPSORT of: pFN21AE6771.seq Check: 5066 from: 1 to: 6394 pFN21AE6771 6394 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4204 278 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3737 1937 Size: 1800 4594 Acc65I G'GTAC_C Cuts at: 2714 3096 3715 2714 Size: 382 619 5393 Fragments arranged by size: 5393 619 382 AclI AA'CG_TT Cuts at: 1563 4521 4894 1563 Size: 2958 373 3063 Fragments arranged by size: 3063 2958 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2946 3270 3670 4457 5841 6109 1632 Size: 1314 324 400 787 1384 268 1917 Fragments arranged by size: 1917 1384 1314 787 400 324 268 AflII C'TTAA_G Cuts at: 820 1017 2978 820 Size: 197 1961 4236 Fragments arranged by size: 4236 1961 197 AflIII A'CryG_T Cuts at: 1203 5293 6270 1203 Size: 4090 977 1327 Fragments arranged by size: 4090 1327 977 AgeI A'CCGG_T Cuts at: 5225 5225 Size: 6394 AhdI GACnn_n'nnGTC Cuts at: 2284 5123 2284 Size: 2839 3555 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6394 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6394 AlwNI CAG_nnn'CTG Cuts at: 2450 4137 5709 2450 Size: 1687 1572 3135 Fragments arranged by size: 3135 1687 1572 ApaI G_GGCC'C Cuts at: 2473 2473 Size: 6394 ApaLI G'TGCA_C Cuts at: 4451 5607 4451 Size: 1156 5238 ApoI r'AATT_y Cuts at: 1493 3703 3929 4081 6190 1493 Size: 2210 226 152 2109 1697 Fragments arranged by size: 2210 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4948 160 Size: 872 3916 1606 Fragments arranged by size: 3916 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 AvaI C'yCGr_G Cuts at: 1870 1943 2217 3477 3719 1870 Size: 73 274 1260 242 4545 Fragments arranged by size: 4545 1260 274 242 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3664 3697 3664 Size: 33 6361 BamHI G'GATC_C Cuts at: 3724 4091 3724 Size: 367 6027 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2099 2344 2432 2714 3096 Size: 325 217 627 312 245 88 282 382 Cuts at: 3096 3269 3715 5170 618 Size: 173 446 1455 1842 Fragments arranged by size: 1842 1455 627 446 382 325 312 282 245 217 173 88 BanII G_rGCy'C Cuts at: 1391 2120 2473 2648 3335 3478 3528 3713 1391 Size: 729 353 175 687 143 50 185 4072 Fragments arranged by size: 4072 729 687 353 185 175 143 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2601 3674 928 Size: 971 702 1073 3648 Fragments arranged by size: 3648 1073 971 702 BbvCI CC'TCA_GC Cuts at: 2775 2775 Size: 6394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4585 4619 1903 Size: 34 2648 34 3678 Fragments arranged by size: 3678 2648 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4286 5496 4286 Size: 1210 5184 BclI T'GATC_A Cuts at: 1548 2842 1548 Size: 1294 5100 BfrBI ATG'CAT Cuts at: 2397 2397 Size: 6394 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3290 5005 Size: 107 122 71 631 605 337 1281 1715 Cuts at: 5005 136 Size: 1525 Fragments arranged by size: 1715 1525 1281 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6389 6389 Size: 6394 BlpI GC'TnA_GC Cuts at: 3811 3811 Size: 6394 Bme1580I G_kGCm'C Cuts at: 1790 2044 2102 2473 3301 4455 5611 1790 Size: 254 58 371 828 1154 1156 2573 Fragments arranged by size: 2573 1156 1154 828 371 254 58 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6394 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2302 2438 2766 Size: 416 53 83 399 24 885 136 328 Cuts at: 2766 2839 3095 4125 5083 442 Size: 73 256 1030 958 1753 Fragments arranged by size: 1753 1030 958 885 416 399 328 256 136 83 73 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6394 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2835 3481 5054 1239 Size: 1596 646 1573 2579 Fragments arranged by size: 2579 1596 1573 646 Bpu10I CC'TnA_GC Cuts at: 2775 2775 Size: 6394 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4519 5384 5682 4519 Size: 865 298 5231 Fragments arranged by size: 5231 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2292 2562 3635 5057 882 Size: 617 793 270 1073 1422 2219 Fragments arranged by size: 2219 1422 1073 793 617 270 BsaAI yAC'GTr Cuts at: 493 1204 4185 493 Size: 711 2981 2702 Fragments arranged by size: 2981 2702 711 BsaBI GATnn'nnATC Cuts at: 2844 6388 2844 Size: 3544 2850 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4201 4583 5218 275 Size: 53 83 186 936 2668 382 635 1451 Fragments arranged by size: 2668 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4827 5225 5499 5646 6057 4827 Size: 398 274 147 411 5164 Fragments arranged by size: 5164 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2655 2685 1178 Size: 30 165 30 1252 30 4887 Fragments arranged by size: 4887 1252 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 2541 2676 2998 3441 1186 Size: 1168 187 135 322 443 4139 Fragments arranged by size: 4139 1168 443 322 187 135 BseYI C'CCAG_C Cuts at: 2095 2103 5597 6093 2095 Size: 8 3494 496 2396 Fragments arranged by size: 3494 2396 496 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2635 3663 1163 Size: 573 899 1028 3894 Fragments arranged by size: 3894 1028 899 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2036 3844 4605 5633 1540 Size: 275 186 35 1808 761 1028 2301 Fragments arranged by size: 2301 1808 1028 761 275 186 35 BsiHKAI G_wGCw'C Cuts at: 2224 4455 4540 5611 2224 Size: 2231 85 1071 3007 Fragments arranged by size: 3007 2231 1071 85 BsmI GAATG_Cn' Cuts at: 2399 3905 3998 2399 Size: 1506 93 4795 Fragments arranged by size: 4795 1506 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2102 2120 2224 2473 2648 3301 Size: 399 254 58 18 104 249 175 653 Cuts at: 3301 3335 3478 3528 3713 4455 4540 5611 1391 Size: 34 143 50 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 653 399 254 249 185 175 143 104 85 58 50 34 18 BspEI T'CCGG_A Cuts at: 6057 6057 Size: 6394 BspHI T'CATG_A Cuts at: 4283 6013 4283 Size: 1730 4664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2306 3749 844 Size: 1462 1443 3489 Fragments arranged by size: 3489 1462 1443 BsrBI CCG'CTC Cuts at: 1394 1823 4281 1394 Size: 429 2458 3507 Fragments arranged by size: 3507 2458 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2800 3006 4889 5063 65 Size: 1183 1552 206 1883 174 1396 Fragments arranged by size: 1883 1552 1396 1183 206 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5038 5225 1700 Size: 110 3228 187 2869 Fragments arranged by size: 3228 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6394 BssHII G'CGCG_C Cuts at: 1925 2065 1925 Size: 140 6254 BssSI C'ACGA_G Cuts at: 4454 5466 4454 Size: 1012 5382 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6394 BstBI TT'CG_AA Cuts at: 5198 5198 Size: 6394 BstEII G'GTnAC_C Cuts at: 1184 2318 1184 Size: 1134 5260 BstXI CCAn_nnnn'nTGG Cuts at: 2725 3280 3448 2725 Size: 555 168 5671 Fragments arranged by size: 5671 555 168 BstYI r'GATC_y Cuts at: 1972 2779 3724 4091 4476 4493 5945 6031 6043 Size: 807 945 367 385 17 1452 86 12 Cuts at: 6043 6389 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 945 807 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2018 2018 Size: 6394 BtgI C'CryG_G Cuts at: 513 1065 2679 513 Size: 552 1614 4228 Fragments arranged by size: 4228 1614 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5655 Fragments arranged by size: 5655 683 56 BtsI GCAGTG_nn' Cuts at: 2640 3910 4704 4724 2640 Size: 1270 794 20 4310 Fragments arranged by size: 4310 1270 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6394 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6359 DraI TTT'AAA Cuts at: 3519 3698 3519 Size: 179 6215 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5401 809 Size: 507 4085 1802 Fragments arranged by size: 4085 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2033 2578 3282 Size: 54 1425 50 275 18 203 545 704 Cuts at: 3282 3398 3450 3841 4730 6359 8 Size: 116 52 391 889 1629 43 Fragments arranged by size: 1629 1425 889 704 545 391 275 203 116 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2033 3841 1537 Size: 275 221 1808 4090 Fragments arranged by size: 4090 1808 275 221 EarI CTCTTCn'nnn_ Cuts at: 1353 2371 2922 3540 4324 1353 Size: 1018 551 618 784 3423 Fragments arranged by size: 3423 1018 784 618 551 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4981 5355 5501 387 Size: 1064 138 3392 374 146 1280 Fragments arranged by size: 3392 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2835 2946 3270 3481 3670 4457 5054 Size: 393 1203 111 324 211 189 787 597 Cuts at: 5054 5841 6109 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1203 787 787 597 393 324 268 211 189 111 EcoNI CCTnn'n_nnAGG Cuts at: 2129 2129 Size: 6394 EcoO109I rG'GnC_Cy Cuts at: 2732 2732 Size: 6394 EcoRI G'AATT_C Cuts at: 3703 4081 3703 Size: 378 6016 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3004 3691 3004 Size: 687 5707 FspI TGC'GCA Cuts at: 4900 4900 Size: 6394 HaeII r_GCGC'y Cuts at: 1678 5541 1678 Size: 3863 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2270 2302 3586 Size: 32 135 32 530 32 136 32 1284 Cuts at: 3586 3618 5035 5067 5109 5141 1373 Size: 32 1417 32 42 32 2626 Fragments arranged by size: 2626 1417 1284 530 136 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3738 3984 669 Size: 988 281 1800 246 3079 Fragments arranged by size: 3079 1800 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6394 HpaI GTT'AAC Cuts at: 3984 3984 Size: 6394 KpnI G_GTAC'C Cuts at: 2718 3100 3719 2718 Size: 382 619 5393 Fragments arranged by size: 5393 619 382 MfeI C'AATT_G Cuts at: 3993 3993 Size: 6394 MluI A'CGCG_T Cuts at: 6270 6270 Size: 6394 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3094 5508 5692 1305 Size: 1789 2414 184 2007 Fragments arranged by size: 2414 2007 1789 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3400 3452 10 Size: 54 1425 343 1568 52 2952 Fragments arranged by size: 2952 1568 1425 343 54 52 MslI CAynn'nnrTG Cuts at: 518 1166 4352 4711 4870 518 Size: 648 3186 359 159 2042 Fragments arranged by size: 3186 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3287 3810 4487 5635 5880 1606 Size: 1681 523 677 1148 245 2120 Fragments arranged by size: 2120 1681 1148 677 523 245 NcoI C'CATG_G Cuts at: 513 1065 2679 513 Size: 552 1614 4228 Fragments arranged by size: 4228 1614 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6394 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6394 NotI GC'GGCC_GC Cuts at: 3841 3841 Size: 6394 NsiI A_TGCA'T Cuts at: 2399 2399 Size: 6394 NspI r_CATG'y Cuts at: 1132 1229 3752 5297 1132 Size: 97 2523 1545 2229 Fragments arranged by size: 2523 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2282 1348 Size: 934 5460 PciI A'CATG_T Cuts at: 5293 5293 Size: 6394 PflMI CCAn_nnn'nTGG Cuts at: 1108 2354 1108 Size: 1246 5148 PmeI GTTT'AAAC Cuts at: 3698 3698 Size: 6394 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4463 4495 5976 6008 4463 Size: 32 1481 32 4849 Fragments arranged by size: 4849 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2732 2732 Size: 6394 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6394 PsiI TTA'TAA Cuts at: 3964 3964 Size: 6394 PspOMI G'GGCC_C Cuts at: 2469 2469 Size: 6394 PstI C_TGCA'G Cuts at: 830 3746 830 Size: 2916 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6394 SalI G'TCGA_C Cuts at: 1936 3736 1936 Size: 1800 4594 SapI GCTCTTCn'nnn_ Cuts at: 2922 2922 Size: 6394 SbfI CC_TGCA'GG Cuts at: 3746 3746 Size: 6394 ScaI AGT'ACT Cuts at: 4642 6354 4642 Size: 1712 4682 SfcI C'TryA_G Cuts at: 826 1045 3742 4877 5558 5749 826 Size: 219 2697 1135 681 191 1471 Fragments arranged by size: 2697 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3290 3290 Size: 6394 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6394 SmaI CCC'GGG Cuts at: 1872 3479 3721 1872 Size: 1607 242 4545 Fragments arranged by size: 4545 1607 242 SmlI C'TyrA_G Cuts at: 820 1017 1943 2978 4498 5399 5661 820 Size: 197 926 1035 1520 901 262 1553 Fragments arranged by size: 1553 1520 1035 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4185 493 Size: 3692 2702 SpeI A'CTAG_T Cuts at: 152 2158 152 Size: 2006 4388 SphI G_CATG'C Cuts at: 1132 3752 1132 Size: 2620 3774 SspI AAT'ATT Cuts at: 5 52 2620 3090 3190 4318 5 Size: 47 2568 470 100 1128 2081 Fragments arranged by size: 2568 2081 1128 470 100 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6394 StyI C'CwwG_G Cuts at: 513 1065 2679 2739 3261 3833 513 Size: 552 1614 60 522 572 3074 Fragments arranged by size: 3074 1614 572 552 522 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2117 4421 4438 4591 4776 5221 325 Size: 1255 537 2304 17 153 185 445 1498 Fragments arranged by size: 2304 1498 1255 537 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4640 6352 96 Size: 274 80 33 51 1443 2663 1712 138 Fragments arranged by size: 2663 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2655 2687 6354 6386 2655 Size: 32 3667 32 2663 Fragments arranged by size: 3667 2663 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6394 XbaI T'CTAG_A Cuts at: 3730 3730 Size: 6394 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3622 3622 Size: 6394 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6394 XmaI C'CCGG_G Cuts at: 1870 3477 3719 1870 Size: 1607 242 4545 Fragments arranged by size: 4545 1607 242 XmnI GAAnn'nnTTC Cuts at: 4523 4523 Size: 6394 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4202 276 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI