(Linear) (Six-base) MAPSORT of: KIEE6771.seq Check: 1854 from: 1 to: 1689 KIEE6771.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. Acc65I G'GTAC_C Cuts at: 0 709 1091 1689 Size: 709 382 598 Fragments arranged by size: 709 598 382 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 941 1265 1665 1689 Size: 941 324 400 24 Fragments arranged by size: 941 400 324 24 AflII C'TTAA_G Cuts at: 0 973 1689 Size: 973 716 AhdI GACnn_n'nnGTC Cuts at: 0 279 1689 Size: 279 1410 AlwNI CAG_nnn'CTG Cuts at: 0 445 1689 Size: 445 1244 ApaI G_GGCC'C Cuts at: 0 468 1689 Size: 468 1221 AvaI C'yCGr_G Cuts at: 0 212 1472 1689 Size: 212 1260 217 Fragments arranged by size: 1260 217 212 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1659 1689 Size: 1659 30 BanI G'GyrC_C Cuts at: 0 94 339 427 709 1091 1264 1689 Size: 94 245 88 282 382 173 425 Fragments arranged by size: 425 382 282 245 173 94 88 BanII G_rGCy'C Cuts at: 0 115 468 643 1330 1473 1523 1689 Size: 115 353 175 687 143 50 166 Fragments arranged by size: 687 353 175 166 143 115 50 BbsI GAAGACnn'nnnn_ Cuts at: 0 596 1669 1689 Size: 596 1073 20 Fragments arranged by size: 1073 596 20 BbvCI CC'TCA_GC Cuts at: 0 770 1689 Size: 770 919 BclI T'GATC_A Cuts at: 0 837 1689 Size: 837 852 BfrBI ATG'CAT Cuts at: 0 392 1689 Size: 392 1297 BglI GCCn_nnn'nGGC Cuts at: 0 1285 1689 Size: 1285 404 Bme1580I G_kGCm'C Cuts at: 0 39 97 468 1296 1689 Size: 39 58 371 828 393 Fragments arranged by size: 828 393 371 58 39 BmrI ACTGGGnnnn_n' Cuts at: 0 297 433 761 834 1090 1689 Size: 297 136 328 73 256 599 Fragments arranged by size: 599 328 297 256 136 73 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 830 1476 1689 Size: 830 646 213 Fragments arranged by size: 830 646 213 Bpu10I CC'TnA_GC Cuts at: 0 770 1689 Size: 770 919 BsaI GGTCTCn'nnnn_ Cuts at: 0 287 557 1630 1689 Size: 287 270 1073 59 Fragments arranged by size: 1073 287 270 59 BsaBI GATnn'nnATC Cuts at: 0 839 1689 Size: 839 850 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 650 680 1689 Size: 650 30 1009 Fragments arranged by size: 1009 650 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 349 536 671 993 1436 1689 Size: 349 187 135 322 443 253 Fragments arranged by size: 443 349 322 253 187 135 BseYI C'CCAG_C Cuts at: 0 90 98 1689 Size: 90 8 1591 Fragments arranged by size: 1591 90 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 630 1658 1689 Size: 630 1028 31 Fragments arranged by size: 1028 630 31 BsiEI CG_ry'CG Cuts at: 0 31 1689 Size: 31 1658 BsiHKAI G_wGCw'C Cuts at: 0 219 1689 Size: 219 1470 BsmI GAATG_Cn' Cuts at: 0 394 1689 Size: 394 1295 Bsp1286I G_dGCh'C Cuts at: 0 39 97 115 219 468 643 1296 1330 Size: 39 58 18 104 249 175 653 34 Cuts at: 1330 1473 1523 1689 Size: 143 50 166 Fragments arranged by size: 653 249 175 166 143 104 58 50 39 34 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 301 1689 Size: 301 1388 BsrDI GCAATG_nn' Cuts at: 0 795 1001 1689 Size: 795 206 688 Fragments arranged by size: 795 688 206 BssHII G'CGCG_C Cuts at: 0 60 1689 Size: 60 1629 BstEII G'GTnAC_C Cuts at: 0 313 1689 Size: 313 1376 BstXI CCAn_nnnn'nTGG Cuts at: 0 720 1275 1443 1689 Size: 720 555 168 246 Fragments arranged by size: 720 555 246 168 BstYI r'GATC_y Cuts at: 0 774 1689 Size: 774 915 Bsu36I CC'TnA_GG Cuts at: 0 13 1689 Size: 13 1676 BtgI C'CryG_G Cuts at: 0 674 1689 Size: 674 1015 BtsI GCAGTG_nn' Cuts at: 0 635 1689 Size: 635 1054 DraI TTT'AAA Cuts at: 0 1514 1689 Size: 1514 175 EaeI y'GGCC_r Cuts at: 0 28 573 1277 1393 1445 1689 Size: 28 545 704 116 52 244 Fragments arranged by size: 704 545 244 116 52 28 EagI C'GGCC_G Cuts at: 0 28 1689 Size: 28 1661 EarI CTCTTCn'nnn_ Cuts at: 0 366 917 1535 1689 Size: 366 551 618 154 Fragments arranged by size: 618 551 366 154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 830 941 1265 1476 1665 1689 Size: 830 111 324 211 189 24 Fragments arranged by size: 830 324 211 189 111 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 124 1689 Size: 124 1565 EcoO109I rG'GnC_Cy Cuts at: 0 727 1689 Size: 727 962 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 999 1686 1689 Size: 999 687 3 Fragments arranged by size: 999 687 3 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 97 129 265 297 1581 1613 1689 Size: 97 32 136 32 1284 32 76 Fragments arranged by size: 1284 136 97 76 32 32 32 KpnI G_GTAC'C Cuts at: 0 713 1095 1689 Size: 713 382 594 Fragments arranged by size: 713 594 382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1089 1689 Size: 1089 600 MscI TGG'CCA Cuts at: 0 1395 1447 1689 Size: 1395 52 242 Fragments arranged by size: 1395 242 52 MspA1I CmG'CkG Cuts at: 0 1282 1689 Size: 1282 407 NcoI C'CATG_G Cuts at: 0 674 1689 Size: 674 1015 NsiI A_TGCA'T Cuts at: 0 394 1689 Size: 394 1295 PasI CC'CwG_GG Cuts at: 0 277 1689 Size: 277 1412 PflMI CCAn_nnn'nTGG Cuts at: 0 349 1689 Size: 349 1340 PpuMI rG'GwC_Cy Cuts at: 0 727 1689 Size: 727 962 PspOMI G'GGCC_C Cuts at: 0 464 1689 Size: 464 1225 SapI GCTCTTCn'nnn_ Cuts at: 0 917 1689 Size: 917 772 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1285 1689 Size: 1285 404 SmaI CCC'GGG Cuts at: 0 1474 1689 Size: 1474 215 SmlI C'TyrA_G Cuts at: 0 973 1689 Size: 973 716 SpeI A'CTAG_T Cuts at: 0 153 1689 Size: 153 1536 SspI AAT'ATT Cuts at: 0 615 1085 1185 1689 Size: 615 470 100 504 Fragments arranged by size: 615 504 470 100 StyI C'CwwG_G Cuts at: 0 674 734 1256 1689 Size: 674 60 522 433 Fragments arranged by size: 674 522 433 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 112 1689 Size: 112 1577 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 650 682 1689 Size: 650 32 1007 Fragments arranged by size: 1007 650 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1617 1689 Size: 1617 72 XmaI C'CCGG_G Cuts at: 0 1472 1689 Size: 1472 217 Enzymes that do cut: Acc65I AcuI AflII AhdI AlwNI ApaI AvaI BaeI BanI BanII BbsI BbvCI BclI BfrBI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssHII BstEII BstXI BstYI Bsu36I BtgI BtsI DraI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI Hin4I KpnI MmeI MscI MspA1I NcoI NsiI PasI PflMI PpuMI PspOMI SapI SfiI SmaI SmlI SpeI SspI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflIII AgeI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaHI BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraIII DrdI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SnaBI SphI SrfI StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI