################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MflI MluI MluNI MroI MscI MslI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 67 CGCG CG^CG AcsI 637 AAATTC [GA]^AATT[CT] AcyI 31 GGCGTC G[GA]^CG[CT]C AfaI 801 GTAC GT^AC ApoI 637 AAATTC [GA]^AATT[CT] AspHI 864 GTGCTC G[AT]GC[AT]^C BbsI 310 GAAGAC GAAGAC(2/6) BcnI 36 CCCGG CC^[GC]GG BmyI 864 GTGCTC G[AGT]GC[ACT]^C BpmI 516 CTCCAG CTGGAG(16/14) BpuAI 310 GAAGAC GAAGAC(2/6) BsaHI 31 GGCGTC G[GA]^CG[CT]C BsaI 521 GGTCTC GGTCTC(1/5) BseRI 400 GAGGAG GAGGAG(10/8) BsiHKAI 864 GTGCTC G[AT]GC[AT]^C BsmAI 522 GTCTC GTCTC(1/5) BsmI 462 GAATGC GAATGC(1/-1) Bsp1286I 864 GTGCTC G[AGT]GC[ACT]^C BspMI 219 ACCTGC ACCTGC(4/8) BsrDI 336 CATTGC GCAATG(2/0) BstF5I 102 GGATG GGATG(2/0) BstUI 67 CGCG CG^CG CfoI 66 GCGC GCG^C DdeI 224 CTAAG C^T[ACGT]AG DpnII 418 GATC ^GATC DsaI 565 CCATGG C^C[GA][CT]GG EcoT14I 565 CCATGG C^C[AT][AT]GG FokI 102 GGATG GGATG(9/13) HgaI 32 GCGTC GACGC(5/10) HhaI 66 GCGC GCG^C Hin1I 31 GGCGTC G[GA]^CG[CT]C HinP1I 66 GCGC G^CGC HphI 169 GGTGA GGTGA(8/7) MaeIII 170 GTGAC ^GT[ACGT]AC MboI 418 GATC ^GATC MfeI 344 CAATTG C^AATTG MspA1I 12 CAGCGG C[AC]G^C[GT]G MunI 344 CAATTG C^AATTG MvnI 67 CGCG CG^CG NciI 36 CCCGG CC^[GC]GG NcoI 565 CCATGG C^CATGG NdeII 418 GATC ^GATC NlaIV 615 GGGACC GG[ACGT]^[ACGT]CC NspI 153 GCATGC [GA]CATG^[CT] PstI 254 CTGCAG CTGCA^G RsaI 801 GTAC GT^AC Sau3AI 418 GATC ^GATC SfcI 254 CTGCAG C^T[GA][CT]AG SmlI 467 CTCAAG C^T[CT][GA]AG SphI 153 GCATGC GCATG^C StyI 565 CCATGG C^C[AT][AT]GG Tsp45I 170 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AvaII 616 GGACC G^G[AT]CC 715 GGACC BsaJI 565 CCATGG C^C[ACGT][ACGT]GG 601 CCAGGG Cac8I 153 GCATGC GC[ACGT]^[ACGT]GC 692 GCTGGC HapII 37 CCGG C^CGG 63 CCGG HpaII 37 CCGG C^CGG 63 CCGG MspI 37 CCGG C^CGG 63 CCGG NdeI 125 CATATG CA^TATG 744 CATATG SfaNI 766 GATGC GCATC(5/9) 772 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI BbvI BsiYI BslI BsmFI BsrI BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII HinfI ItaI MboII MnlI MseI MvaI MwoI NlaIII Sau96I ScrFI TfiI Tru9I TseI Tsp509I TspRI