################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccI 402 GTATAC GT^[AC][GT]AC AfaI 198 GTAC GT^AC AlwI 348 GATCC GGATC(4/5) AvaII 41 GGTCC G^G[AT]CC BanII 143 GAGCCC G[GA]GC[CT]^C BfaI 532 CTAG C^TAG BglI 295 GCCCGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 143 GAGCCC G[AGT]GC[ACT]^C BsaI 440 GGTCTC GGTCTC(1/5) BsgI 8 CTGCAC GTGCAG(16/14) Bsp1286I 143 GAGCCC G[AGT]GC[ACT]^C BsrFI 38 GCCGGT [GA]^CCGG[CT] Bst1107I 402 GTATAC GTA^TAC BstXI 485 CCAAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 347 AGATCC [GA]^GATC[CT] BstZ17I 402 GTATAC GTA^TAC Cac8I 71 GCAAGC GC[ACGT]^[ACGT]GC Cfr10I 38 GCCGGT [GA]^CCGG[CT] MaeI 532 CTAG C^TAG MflI 347 AGATCC [GA]^GATC[CT] MseI 637 TTAA T^TAA NdeI 426 CATATG CA^TATG NlaIII 615 CATG CATG^ PflMI 309 CCAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 538 GACTC GAGTC(4/5) RsaI 198 GTAC GT^AC SmlI 765 CTCAAG C^T[CT][GA]AG TfiI 699 GAATC G^A[AT]TC Tru9I 637 TTAA T^TAA Van91I 309 CCAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 425 CCATATGTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 347 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BbsI 535 GAAGAC GAAGAC(2/6) 725 GAAGAC BcnI 252 CCGGG CC^[GC]GG 333 CCCGG BpuAI 535 GAAGAC GAAGAC(2/6) 725 GAAGAC BsaJI 146 CCCTGG C^C[ACGT][ACGT]GG 716 CCCTGG BspMI 525 ACCTGC ACCTGC(4/8) 579 GCAGGT BsrBI 48 CCGCTC CCGCTC(-3/-3) 87 GAGCGG EarI 443 CTCTTC CTCTTC(1/4) 732 GAAGAG HaeII 29 AGCGCC [GA]GCGC^[CT] 124 AGCGCC HinfI 538 GACTC G^A[ACGT]TC 699 GAATC HphI 343 GGTGA GGTGA(8/7) 720 GGTGA Ksp632I 443 CTCTTC CTCTTC(1/4) 732 GAAGAG MslI 183 CACACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 408 CACAGAGATG NciI 252 CCGGG CC^[GC]GG 333 CCCGG PstI 82 CTGCAG CTGCA^G 382 CTGCAG PvuII 237 CAGCTG CAG^CTG 379 CAGCTG SfaNI 162 GATGC GCATC(5/9) 414 GATGC SfcI 82 CTGCAG C^T[GA][CT]AG 382 CTGCAG TaqI 55 TCGA T^CGA 456 TCGA Tsp509I 314 AATT ^AATT 504 AATT TspRI 385 CAGTG CA[GC]TG[ACGT][ACGT]^ 603 CACTG TthHB8I 55 TCGA T^CGA 456 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI BbvI BseRI BsiYI BslI BsmAI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TseI