(Linear) (Six-base) MAPSORT of: KIEE3089.seq Check: 3742 from: 1 to: 771 KIEE3089.orf With 182 enzymes: SgfI * March 22, 2012 16:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 534 771 Size: 534 237 AccI GT'mk_AC Cuts at: 0 403 771 Size: 403 368 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 771 Size: 430 341 AleI CACnn'nnGTG Cuts at: 0 187 771 Size: 187 584 BanII G_rGCy'C Cuts at: 0 147 771 Size: 147 624 BbsI GAAGACnn'nnnn_ Cuts at: 0 542 732 771 Size: 542 190 39 Fragments arranged by size: 542 190 39 BglI GCCn_nnn'nGGC Cuts at: 0 301 771 Size: 301 470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 750 771 Size: 750 21 BsaI GGTCTCn'nnnn_ Cuts at: 0 446 771 Size: 446 325 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 42 523 531 771 Size: 42 481 8 240 Fragments arranged by size: 481 240 42 8 BseYI C'CCAG_C Cuts at: 0 34 435 771 Size: 34 401 336 Fragments arranged by size: 401 336 34 Bsp1286I G_dGCh'C Cuts at: 0 147 771 Size: 147 624 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 534 570 771 Size: 534 36 201 Fragments arranged by size: 534 201 36 BsrBI CCG'CTC Cuts at: 0 50 89 771 Size: 50 39 682 Fragments arranged by size: 682 50 39 BsrFI r'CCGG_y Cuts at: 0 38 771 Size: 38 733 BstXI CCAn_nnnn'nTGG Cuts at: 0 492 771 Size: 492 279 BstYI r'GATC_y Cuts at: 0 347 771 Size: 347 424 BstZ17I GTA'TAC Cuts at: 0 404 771 Size: 404 367 BtsI GCAGTG_nn' Cuts at: 0 391 771 Size: 391 380 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 576 611 771 Size: 576 35 160 Fragments arranged by size: 576 160 35 EarI CTCTTCn'nnn_ Cuts at: 0 449 727 771 Size: 449 278 44 Fragments arranged by size: 449 278 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 271 771 Size: 271 500 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 771 Size: 430 341 HaeII r_GCGC'y Cuts at: 0 33 128 771 Size: 33 95 643 Fragments arranged by size: 643 95 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 194 226 529 561 771 Size: 194 32 303 32 210 Fragments arranged by size: 303 210 194 32 32 MslI CAynn'nnrTG Cuts at: 0 187 412 771 Size: 187 225 359 Fragments arranged by size: 359 225 187 MspA1I CmG'CkG Cuts at: 0 81 113 239 381 771 Size: 81 32 126 142 390 Fragments arranged by size: 390 142 126 81 32 NdeI CA'TA_TG Cuts at: 0 427 771 Size: 427 344 PflMI CCAn_nnn'nTGG Cuts at: 0 315 771 Size: 315 456 PstI C_TGCA'G Cuts at: 0 86 386 771 Size: 86 300 385 Fragments arranged by size: 385 300 86 PvuII CAG'CTG Cuts at: 0 239 381 771 Size: 239 142 390 Fragments arranged by size: 390 239 142 SfcI C'TryA_G Cuts at: 0 82 382 771 Size: 82 300 389 Fragments arranged by size: 389 300 82 SmlI C'TyrA_G Cuts at: 0 765 771 Size: 765 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 222 771 Size: 222 549 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 432 771 Size: 432 339 Enzymes that do cut: AarI AccI AcuI AleI BanII BbsI BglI BpuEI BsaI BseRI BseYI Bsp1286I BspMI BsrBI BsrFI BstXI BstYI BstZ17I BtsI CspCI EarI EciI Eco57MI HaeII Hin4I MslI MspA1I NdeI PflMI PstI PvuII SfcI SmlI TaqII XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgI BtgZI ClaI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI