################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsiEI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI Tsp45I Tth111I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccII 14 CGCG CG^CG AcsI 258 AAATTC [GA]^AATT[CT] AlwI 403 GATCC GGATC(4/5) AlwNI 337 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 258 AAATTC [GA]^AATT[CT] AvaI 647 CTCGAG C^[CT]CG[GA]G BanII 704 GAGCTC G[GA]GC[CT]^C BbsI 151 GAAGAC GAAGAC(2/6) BciVI 625 GTATCC GTATCC(6/5) BfaI 332 CTAG C^TAG BpmI 672 CTGGAG CTGGAG(16/14) BpuAI 151 GAAGAC GAAGAC(2/6) BsaAI 25 TACGTG [CT]AC^GT[GA] BsgI 323 GTGCAG GTGCAG(16/14) BsiWI 23 CGTACG C^GTACG BsmAI 575 GAGAC GTCTC(1/5) BsmFI 339 GTCCC GGGAC(10/14) BsmI 465 GAATGC GAATGC(1/-1) BsoBI 647 CTCGAG C^[CT]CG[GA]G BspMI 488 GCAGGT ACCTGC(4/8) BsrDI 351 CATTGC GCAATG(2/0) BssKI 563 CCAGG ^CC[ACGT]GG BstNI 563 CCAGG CC^[AT]GG BstUI 14 CGCG CG^CG BstXI 629 CCATTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 403 GATC ^GATC DraI 766 TTTAAA TTT^AAA EarI 513 CTCTTC CTCTTC(1/4) EcoNI 263 CCTTTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 563 CCAGG ^CC[AT]GG HgaI 12 GACGC GACGC(5/10) HincII 684 GTTGAC GT[CT]^[GA]AC HindII 684 GTTGAC GT[CT]^[GA]AC HphI 843 TCACC GGTGA(8/7) Ksp632I 513 CTCTTC CTCTTC(1/4) MaeI 332 CTAG C^TAG MaeII 26 ACGT A^CGT MboI 403 GATC ^GATC MslI 630 CATTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 326 CAGCTG C[AC]G^C[GT]G MvaI 563 CCAGG CC^[AT]GG MvnI 14 CGCG CG^CG MwoI 706 GCTCTGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 453 CATATG CA^TATG NdeII 403 GATC ^GATC NlaIV 307 GGAACC GG[ACGT]^[ACGT]CC NspI 533 GCATGC [GA]CATG^[CT] PaeR7I 647 CTCGAG C^TCGAG PflMI 814 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 426 CTGCAG CTGCA^G PvuII 326 CAGCTG CAG^CTG SacI 704 GAGCTC GAGCT^C Sau3AI 403 GATC ^GATC ScrFI 563 CCAGG CC^[ACGT]GG SfcI 426 CTGCAG C^T[GA][CT]AG SmlI 647 CTCGAG C^T[CT][GA]AG SphI 533 GCATGC GCATG^C SspI 717 AATATT AAT^ATT TfiI 825 GAATC G^A[AT]TC Van91I 814 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 647 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 15 GCGG CCGC(-3/-1) 378 GCGG AluI 327 AGCT AG^CT 705 AGCT AspHI 40 GTGCTC G[AT]GC[AT]^C 704 GAGCTC BmyI 40 GTGCTC G[AGT]GC[ACT]^C 704 GAGCTC BseRI 107 GAGGAG GAGGAG(10/8) 781 GAGGAG BsiHKAI 40 GTGCTC G[AT]GC[AT]^C 704 GAGCTC BsiYI 263 CCTTTATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 814 CCAACAACTGG BslI 263 CCTTTATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 814 CCAACAACTGG Bsp1286I 40 GTGCTC G[AGT]GC[ACT]^C 704 GAGCTC BstF5I 393 GGATG GGATG(2/0) 566 GGATG Cac8I 54 GCTGGC GC[ACGT]^[ACGT]GC 533 GCATGC Cfr13I 30 GGCCC G^G[ACGT]CC 639 GGGCC FokI 393 GGATG GGATG(9/13) 566 GGATG MaeIII 242 GTAAC ^GT[ACGT]AC 437 GTTAC MseI 479 TTAA T^TAA 767 TTAA Sau96I 30 GGCCC G^G[ACGT]CC 639 GGGCC Tru9I 479 TTAA T^TAA 767 TTAA TspRI 602 CACTG CA[GC]TG[ACGT][ACGT]^ 670 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AfaI BbvI BsrI Fnu4HI HaeIII HinfI ItaI MboII MnlI NlaIII PleI RsaI TaqI TseI Tsp509I TthHB8I